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Identification and application of bacterial volatiles to attract a generalist aphid parasitoid: from laboratory to greenhouse assays
Goelen, Tim Vuts, József Sobhy, Islam S. Wäckers, Felix Caulfield, John C. Birkett, Michael A. Rediers, Hans Jacquemyn, Hans Lievens, Bart
Goelen, Tim
Vuts, József
Sobhy, Islam S.
Wäckers, Felix
Caulfield, John C.
Birkett, Michael A.
Rediers, Hans
Jacquemyn, Hans
Lievens, Bart
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2020-09-25
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Article - AAM
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Abstract
BACKGROUND: Recent studies have shown that microorganisms emit volatile compounds that affect insect behaviour. However, it remains largely unclear whether microbes can be exploited as a source of attractants to improve biological control of insect pests. In this study, we used a combination of coupled gas chromatography-electroantennography (GC-EAG) and Y-tube olfactometer bioassays to identify attractive compounds in the volatile extracts of three bacterial strains that are associated with the habitat of the generalist aphid parasitoid Aphidius colemani, and to create mixtures of synthetic compounds to find attractive blends for A. colemani. Subsequently, the most attractive blend was evaluated in two-choice cage experiments under greenhouse conditions.
RESULTS: GC-EAG analysis revealed 20 compounds that were linked to behaviourally attractive bacterial strains. A mixture of two EAG-active compounds, styrene and benzaldehyde applied at a respective dose of 1 μg and 10 ng, was more attractive than the single compounds or the culture medium of the bacteria in Y-tube olfactometer bioassays. Application of this synthetic mixture under greenhouse conditions resulted in significant attraction of the parasitoids, and outperformed application of the bacterial culture medium.
CONCLUSION: Compounds isolated from bacterial blends were capable of attracting parasitoids both in laboratory and greenhouse assays, indicating that microbial cultures are an effective source of insect attractants. This opens new opportunities to attract and retain natural enemies of pest species and to enhance biological pest control.
Citation
Goelen, T., Vuts, J., Sobhy, I. S., Wäckers, F., Caulfield, J. C., Birkett, M. A., Rediers, H., Jacquemyn, H., & Lievens, B. (2021). Identification and application of bacterial volatiles to attract a generalist aphid parasitoid: from laboratory to greenhouse assays. Pest Management Science, 77(2), 930–938. https://doi.org/https://doi.org/10.1002/ps.6102
Publisher
Wiley
Journal
Pest Management Science
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Article
Language
en
Description
This is the peer reviewed version of the following article: [Goelen, T., Vuts, J., Sobhy, I. S., Wäckers, F., Caulfield, J. C., Birkett, M. A., Rediers, H., Jacquemyn, H., & Lievens, B. (2021). Identification and application of bacterial volatiles to attract a generalist aphid parasitoid: from laboratory to greenhouse assays. Pest Management Science, 77(2), 930–938], which has been published in final form at [https://doi.org/10.1002/ps.6102]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
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1526-498X
EISSN
1526-4998
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The authors would like to thank the Flemish Fund for Scientific Research (FWO; 1S15116316N) and Flanders Innovation & Entrepreneurship (VLAIO; HBC.2018/2022) for financial support. Further, the authors are grateful to Dries Belmans and Francine van Neerbos for help with the experiments, and Sofie Bossaert for help with the figures.