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Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance

Tickle, Jacqueline A.
; 
Jenkins, Stuart I.
; 
Polyak, Boris
; 
Pickard, Mark R.
; 
Chari, Divya M.
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Affiliation
Keele University; Drexel University College of Medicine
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Publication Date
2016-01-20
Submitted Date
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Chester Medical School
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Abstract
AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.
Citation
Tickle, J. A., Jenkins, S. I., Polyark, B., Pickard, M. R., & Chari, D. M. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 11(4), 345-358. http://dx.doi.org/10.2217/nnm.15.202
Publisher
Future Medicine
Journal
Nanomedicine
Research Unit
URI
http://hdl.handle.net/10034/604463
DOI
10.2217/nnm.15.202
PubMed ID
26785794
PubMed Central ID
Type
Article
Language
en
Description
Series/Report no.
ISSN
1743-5889
EISSN
1748-6963
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Sponsors
BBSRC, UK; National Heart, Lung and Blood Institute and Drexel University College of Medicine Clinical & Translational Research Institute, CTRIUSA Award Number R01HL107771; EPSRC E-TERM Landscape Fellowship (EP/I017801/1)
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http://www.futuremedicine.com/doi/10.2217/nnm.15.202
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