Minichromosome maintenance protein in myxofibrosarcoma
| dc.contributor.author | Sington, James D. | * |
| dc.contributor.author | Freeman, Alex | * |
| dc.contributor.author | Morris, Lesley S. | * |
| dc.contributor.author | Vowler, Sarah L. | * |
| dc.contributor.author | Arch, Barbara N. | * |
| dc.contributor.author | Fisher, Cyril | * |
| dc.contributor.author | Coleman, Nicholas | * |
| dc.date.accessioned | 2010-04-16T08:19:11Z | |
| dc.date.available | 2010-04-16T08:19:11Z | |
| dc.date.issued | 2004-02-01 | |
| dc.identifier.citation | Modern Pathology, 17(2), 2004, pp. 235-240 | |
| dc.identifier.issn | 0893-3952 | en |
| dc.identifier.issn | 1530-0285 | en |
| dc.identifier.doi | 10.1038/modpathol.3800044 | |
| dc.identifier.uri | http://hdl.handle.net/10034/96635 | |
| dc.description | This article is not available from CheserRep. The full-text can be accessed at http://www.nature.com/modpathol/journal/v17/n2/full/3800044a.html | |
| dc.description.abstract | Histopathological assessment of myxofibrosarcoma may be difficult, especially on the basis of a small core biopsy, which enables only a crude evaluation of grade and prognosis. Hypothesis - that determination of cell cycle state may assist in the diagnostic assessment of myxofibrosarcoma. 51 cases of high-grade (n=20), intermediate-grade (n=21), and low-grade (n=10) myxofibrosarcomas were studied, as well as nine cases of benign myxoma. Cell cycle state within tumors was determined by immunostaining for the recently described marker minichromosome maintenance protein 2 (MCM2), together with Ki67. Labelling indices for both markers were correlated with tumor grade, mitotic index, and time to first recurrence. The MCM2 labelling indices were significantly higher than the Ki-67 labelling indices. Both indices showed a significant correlation with the mitotic index and both showed significant increases with increasing grade of myxofibrosarcoma. The MCM2 labelling index (but not the Ki67 labelling index) showed a significant inverse exponential correlation with the time to first recurrence. Myxoid and cellular areas showed no difference in the MCM2 and Ki-67 labeling index, suggesting that clinically useful information could be obtained from any component of a myxofibrosarcoma sampled in a needle biopsy and/or cytological specimen. We therefore suggest that assessment of cell cycle state may be a useful diagnostic adjunct in the histopathological assessment of myxofibrosarcoma, by enabling more accurate determination of grade and prediction of outcome. | |
| dc.description.sponsorship | This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies. | |
| dc.language.iso | en | en |
| dc.publisher | Nature | |
| dc.relation.url | http://www.nature.com/modpathol/index.html | en |
| dc.subject | myxofibrosarcoma | en |
| dc.subject | recurrence | en |
| dc.subject | prognosis | en |
| dc.subject | MCM2 | en |
| dc.subject | Ki67 | en |
| dc.title | Minichromosome maintenance protein in myxofibrosarcoma | en |
| dc.type | Article | en |
| dc.contributor.department | University of Cambridge ; Royal Marsden Hospital (Fisher) | |
| dc.identifier.journal | Modern Pathology | en |
| dc.date.accepted | 2003-08-18 | |
| html.description.abstract | Histopathological assessment of myxofibrosarcoma may be difficult, especially on the basis of a small core biopsy, which enables only a crude evaluation of grade and prognosis. Hypothesis - that determination of cell cycle state may assist in the diagnostic assessment of myxofibrosarcoma. 51 cases of high-grade (n=20), intermediate-grade (n=21), and low-grade (n=10) myxofibrosarcomas were studied, as well as nine cases of benign myxoma. Cell cycle state within tumors was determined by immunostaining for the recently described marker minichromosome maintenance protein 2 (MCM2), together with Ki67. Labelling indices for both markers were correlated with tumor grade, mitotic index, and time to first recurrence. The MCM2 labelling indices were significantly higher than the Ki-67 labelling indices. Both indices showed a significant correlation with the mitotic index and both showed significant increases with increasing grade of myxofibrosarcoma. The MCM2 labelling index (but not the Ki67 labelling index) showed a significant inverse exponential correlation with the time to first recurrence. Myxoid and cellular areas showed no difference in the MCM2 and Ki-67 labeling index, suggesting that clinically useful information could be obtained from any component of a myxofibrosarcoma sampled in a needle biopsy and/or cytological specimen. We therefore suggest that assessment of cell cycle state may be a useful diagnostic adjunct in the histopathological assessment of myxofibrosarcoma, by enabling more accurate determination of grade and prediction of outcome. |