AuthorsSington, James D.
Morris, Lesley S.
Vowler, Sarah L.
Arch, Barbara N.
AffiliationUniversity of Cambridge ; Royal Marsden Hospital (Fisher)
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AbstractHistopathological assessment of myxofibrosarcoma may be difficult, especially on the basis of a small core biopsy, which enables only a crude evaluation of grade and prognosis. Hypothesis - that determination of cell cycle state may assist in the diagnostic assessment of myxofibrosarcoma. 51 cases of high-grade (n=20), intermediate-grade (n=21), and low-grade (n=10) myxofibrosarcomas were studied, as well as nine cases of benign myxoma. Cell cycle state within tumors was determined by immunostaining for the recently described marker minichromosome maintenance protein 2 (MCM2), together with Ki67. Labelling indices for both markers were correlated with tumor grade, mitotic index, and time to first recurrence. The MCM2 labelling indices were significantly higher than the Ki-67 labelling indices. Both indices showed a significant correlation with the mitotic index and both showed significant increases with increasing grade of myxofibrosarcoma. The MCM2 labelling index (but not the Ki67 labelling index) showed a significant inverse exponential correlation with the time to first recurrence. Myxoid and cellular areas showed no difference in the MCM2 and Ki-67 labeling index, suggesting that clinically useful information could be obtained from any component of a myxofibrosarcoma sampled in a needle biopsy and/or cytological specimen. We therefore suggest that assessment of cell cycle state may be a useful diagnostic adjunct in the histopathological assessment of myxofibrosarcoma, by enabling more accurate determination of grade and prediction of outcome.
CitationModern Pathology, 17(2), 2004, pp. 235-240
DescriptionThis article is not available from CheserRep. The full-text can be accessed at http://www.nature.com/modpathol/journal/v17/n2/full/3800044a.html
SponsorsThis article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.