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    Evaluation of heat shock protein 70 as a biomarker of environmental stress in Fucus serratus and Lemna minor

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    Authors
    Ireland, H. Elyse
    Harding, Steve J.
    Bonwick, Graham A.
    Jones, Michael
    Smith, Christopher J.
    Williams, John H. H.
    Affiliation
    University College Chester
    Publication Date
    2004-03
    
    Metadata
    Show full item record
    Abstract
    Heat shock proteins (Hsps) are known to be induced in response to short-term stress. The present study aimed to evaluate the potential of Hsp70 as a biomarker of stress produced by increased temperature, osmotic pressure, and exposure to cadmium and sodium chloride in marine macroalgae and fresh water plant species. An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed with a working range of 0.025-10 μg ml-1 using a monoclonal antibody raised against purified Hsp70 of Phaseolus aureus (mung bean). Fucus serratus (toothed wrack), Chondrus crispus (Stackhouse or Carrageen moss), Ulva lactuca (sea lettuce) and Lemna minor (common duckweed) sample extracts were stressed for up to 24 h and then tested in the IC-ELISA. The presence of Hsp70 and cross-reactivity of the monoclonal antibody was confirmed by Western blot. The heat shock response was confirmed in each species using a 2-h 42°C treatment. Following heat shock, Hsp70 concentrations increased to a peak at 2 h (F. serratus) or 4 h (L. minor), after which concentrations decreased. Osmotic and cadmium stresses also resulted in elevated Hsp70 concentrations in samples of F. serratus and L. minor when compared with unstressed controls. In both, osmotic and metal stress, the production of Hsp70 increased to a maximum and subsequently decreased as the stressor levels increased. Results suggest that Hsp70 IC-ELISA could potentially be applied to the detection of stress in these aquatic species, although it would probably be most effective when used in conjunction with other measurements to provide a stressor-specific biomarker profile or fingerprint.
    Citation
    Biomarkers, 2004, 9(2), pp. 139-155
    Publisher
    Taylor & Francis
    Journal
    Biomarkers
    URI
    http://hdl.handle.net/10034/70454
    DOI
    10.1080/13547500410001732610
    Additional Links
    http://www.informaworld.com/smpp/title~content=t713693137~db=all
    Type
    Article
    Language
    en
    Description
    This article is not available through ChesterRep.
    ISSN
    1354750X
    13665804
    Sponsors
    This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.
    ae974a485f413a2113503eed53cd6c53
    10.1080/13547500410001732610
    Scopus Count
    Collections
    Biological Sciences

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