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Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration

dc.contributor.authorHurley, Ian P.*
dc.contributor.authorColeman, Robert C.*
dc.contributor.authorIreland, H. Elyse*
dc.contributor.authorWilliams, John H. H.*
dc.date.accessioned2009-06-15T11:08:37Z
dc.date.available2009-06-15T11:08:37Z
dc.date.issued2004-03
dc.identifier.citationHurley, I. P., Coleman, R. C., Ireland, H. E., & Williams, J. H. H. (2004). Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration. Journal of Dairy Science, 87(3), 543-549. https://doi.org/10.3168/jds.S0022-0302(04)73195-1
dc.identifier.doi10.3168/jds.S0022-0302(04)73195-1
dc.identifier.urihttp://hdl.handle.net/10034/70436
dc.descriptionThis article is not available through ChesterRep. It is available at http://download.journals.elsevierhealth.com/pdfs/journals/0022-0302/PIIS0022030204731951.pdf
dc.description.abstractThe aim of this work was to develop an assay capable of detecting adulteration of high premium milk with milk from cheaper sources. An indirect, competitive ELISA was developed for the rapid detection of cows’ milk in the milk of goat, sheep, and buffalo. The assay uses a monoclonal antibody produced against bovine IgG. This antibody recognizes a species-specific epitope on the heavy chain of both bovine IgG1 and IgG2. A peroxidase-conjugated anti-mouse IgG antibody was used to detect bound monoclonal antibody and subsequent enzymatic conversion of substrate resulted in clear differences in absorbance when assaying different mixtures of milks adulterated with cows’ milk. Once optimized, the ELISA was found to be highly specific. Detection limits of the assay are 1.0 µg/mL of bovine IgG, or 0.1% (vol/vol) adulteration with cows’ milk. The assay was highly reproducible (CV < 10%) and performed equally well when used to detect bovine IgG in mixtures with the 3 types of milk tested. The ELISA performance makes it suitable for development as a kit, for use in the field as a high throughput screening ELISA.
dc.description.sponsorshipThis article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.
dc.language.isoenen
dc.publisherAmerican Dairy Science Association
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0022030204731951?via%3Dihuben
dc.subjectmilk adulterationen
dc.subjectELISAen
dc.subjectIgGen
dc.titleMeasurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulterationen
dc.typeArticle
dc.contributor.departmentUniversity College Chester
dc.identifier.journalJournal of Dairy Scienceen
dc.identifier.volume87
dc.date.accepted2003-10-07
dc.source.issue3
dc.source.beginpage543-549
html.description.abstractThe aim of this work was to develop an assay capable of detecting adulteration of high premium milk with milk from cheaper sources. An indirect, competitive ELISA was developed for the rapid detection of cows’ milk in the milk of goat, sheep, and buffalo. The assay uses a monoclonal antibody produced against bovine IgG. This antibody recognizes a species-specific epitope on the heavy chain of both bovine IgG1 and IgG2. A peroxidase-conjugated anti-mouse IgG antibody was used to detect bound monoclonal antibody and subsequent enzymatic conversion of substrate resulted in clear differences in absorbance when assaying different mixtures of milks adulterated with cows’ milk. Once optimized, the ELISA was found to be highly specific. Detection limits of the assay are 1.0 µg/mL of bovine IgG, or 0.1% (vol/vol) adulteration with cows’ milk. The assay was highly reproducible (CV < 10%) and performed equally well when used to detect bovine IgG in mixtures with the 3 types of milk tested. The ELISA performance makes it suitable for development as a kit, for use in the field as a high throughput screening ELISA.


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