Recent Submissions

  • Corrigendum to “Scientific opinion on Biphenyl-2-ol and Sodium 2-biphenylolate used in cosmetic products (CAS/EC No. 90-43-7/201-993-5 and 132-27-4/205-055-6)– SCCS/1669/24” [NAM Journal Volume 1, 2025, 100035]

    Bernauer, Ulrike; Bodin, Laurent; Chaudhry, Qasim; Coenraads, Pieter Jan; Ezendam, Janine; Gaffet, Eric; Galli, Corrado L.; Panteri, Eirini; Rogiers, Vera; Rousselle, Christophe; et al. (Elsevier, 2025-10-14)
    Corrigendum
  • Eating well when living with an intellectual disability—Exploring the carer: client relationship in residential settings

    Ellahi, Basma; Özdemir, Aslıhan; Chapman, Hazel M.; Hall, Rebecca; University of Chester; Hacettepe University; University of Salford (Wiley, 2025-11-30)
    Background: Individuals with intellectual disabilities face increased risks of obesity and health issues. Carers in residential settings play a crucial role in shaping their dietary habits. This study explores how carers influence eating behaviours to identify strategies for healthy eating. Method: Seventeen semi-structured interviews were conducted with carers from three community homes. Thematic analysis identified three key themes: (i) whose responsibility is it?; (ii) food autonomy and choice in the context of caring relationships; (iii) opportunities for working together to support dietary choices. Results: Carers strive to encourage healthy eating while respecting residents' autonomy, particularly in those with cognitive impairments or on psychotropic medications. They use strategies like rapport-building, personalised care, and nutrition education. However, these efforts are limited by gaps in knowledge, time constraints, and variation in application and impact. Conclusions: Findings highlight the practice gap and the need for better training and resources to support carers in promoting healthy food choices while respecting residents' autonomy.
  • A generic theory of change-based framework with core indicators for monitoring the effectiveness of large-scale food fortification programs in low- and middle-income countries

    Rodas-Moya, Santiago; Giudici, Francesca M.; Owolabi, Adedotun Joshua; Samuel, Folake; Kodish, Stephen R.; Lachat, Carl; Abreu, Taymara C.; van het Hof, Karin H.; Osendarp, Saskia J. M.; Brouwer, Inge D.; et al. (Frontiers Media, 2023-06-22)
    Large-scale food fortification (LSFF) programs are widely implemented in low- and middle-income countries (LMIC) to alleviate micronutrient deficiencies. However, these programs may not achieve the desired impact due to poor design or bottlenecks in program implementation. Monitoring and evaluation (M&E) frameworks and a set of agreed indicators can help to benchmark progress and to strengthen the evidence-base of effectiveness in a standardized way. We aimed to formulate recommendations towards core indicators for evaluating the effectiveness of LSFF programs with their associated metrics, methods, and tools (IMMT). For this, we used a multi-method iterative approach, including a mapping review of the literature, semi-structured interviews with international experts, compilation of a generic Theory of Change (ToC) framework for LSFF program delivery, and selection of IMMT for M&E of LSFF programs at key stages along the ToC delivery framework. Lastly, we conducted exploratory, qualitative interviews with key informants in Nigeria to explore experiences and perceptions related to the implementation of LSFF programs in Nigeria's context, and their opinion towards the proposed set of core IMMT. The literature search resulted in 14 published and 15 grey literature documents, from which we extracted a total of 41 indicators. Based on the available literature and interviews with international experts, we mapped a ToC delivery framework and selected nine core indicators at the output, outcome and impact level for M&E of the effectiveness of LSFF programs. Key informants in Nigeria revealed that the main bottlenecks for implementation of the proposed IMMT are related to the lack of technical capacity, equipment, laboratory infrastructure, and financial resources. In conclusion, we propose a set of nine core indicators for enabling comprehensive M&E of the effectiveness of LSFF programs in LMIC. This proposed set of core indicators can be used for further evaluation, harmonization and integration in national and international protocols for M&E of LSFF programs.
  • Effect of a fortified dairy-based drink on micronutrient status, growth, and cognitive development of Nigerian toddlers- a dose-response study

    Senbanjo, Idowu Odunayo; Owolabi, Adedotun Joshua; Oshikoya, Kazeem Adeola; Hageman, Jeske H. J.; Adeniyi, Yetunde; Samuel, Folake; Melse-Boonstra, Alida; Schaafsma, Anne; Lagos State University College of Medicine; Wageningen University; FrieslandCampina, The Netherlands; University College Hospital, Nigeria; University of Ibadan (Frontiers Media, 2022-04-27)
    Malnutrition results in a high prevalence of stunting, underweight, and micronutrient deficiencies. This study investigated the effect of a multi-nutrient fortified dairy-based drink on micronutrient status, growth, and cognitive development in malnourished [height-for-age z-score (HAZ) and/or weight-for-age z-score (WAZ) &lt; -1 SD and &gt;-3 SD] Nigerian toddlers (<i>n</i> = 184, 1-3 years). The product was provided in different daily amounts (200, 400, or 600 ml) for 6 months. At baseline and endline, venous blood and urine samples were collected to determine micronutrient status. Bodyweight, height, waist, and head circumference were measured, and corresponding Z-scores were calculated. The Bayley-III Screening Test was used to classify the cognitive development of the children. In a modified per-protocol (PP) population, the highest prevalence's of micronutrient deficiencies were found for vitamin A (35.5%) and selenium (17.9%). At endline, there were no significant improvements in iodine, zinc, vitamin B12, and folate status in any of the three groups. Regarding vitamin D status (25OHD), consumption of 600 and 400 ml resulted in an improved status as compared to baseline, and in a difference between the 600- and 200-ml groups. Consumption of 600 ml also increased vitamin A and selenium status as compared to baseline, but no differences were found between groups. Within the groups, WAZ, weight-for-height z-score (WHZ), and BMI-for-age z-score (BAZ) improved, but without differences between the groups. For HAZ, only the 600 ml group showed improvement within the group, but it was not different between groups. For the absolute weight, height, and head circumference only trends for differences between groups were indicated. Cognition results did not differ between the groups. Within groups, all showed a decline in the per cent of competent children for receptive language. To study the effects of a nutritional intervention on linear growth and cognition, a longer study duration might be necessary. Regarding the improvement of micronutrient status, 600 ml of fortified dairy-based drink seems most effective.
  • Multi-nutrient fortified dairy-based drink reduces anaemia without observed adverse effects on gut microbiota in anaemic malnourished Nigerian toddlers: A randomised dose–response study

    Owolabi, Adedotun Joshua; Senbanjo, Idowu Odunayo; Oshikoya, Kazeem Adeola; Boekhorst, Jos; Eijlander, Robyn T.; Kortman, Guus A. M.; Hageman, Jeske H. J.; Samuel, Folake; Melse-Boonstra, Alida; Schaafsma, Anne; et al. (MDPI, 2021-05-06)
    Prevalence of anaemia among Nigerian toddlers is reported to be high, and may cause significant morbidity, affects brain development and function, and results in weakness and fatigue. Although, iron fortification can reduce anaemia, yet the effect on gut microbiota is unclear. This open-label randomised study in anaemic malnourished Nigerian toddlers aimed to decrease anaemia without affecting pathogenic gut bacteria using a multi-nutrient fortified dairy-based drink. The test product was provided daily in different amounts (200, 400 or 600 mL, supplying 2.24, 4.48 and 6.72 mg of elemental iron, respectively) for 6 months. Haemoglobin, ferritin, and C-reactive protein concentrations were measured to determine anaemia, iron deficiency (ID) and iron deficiency anaemia (IDA) prevalence. Faecal samples were collected to analyse gut microbiota composition. All three dosages reduced anaemia prevalence, to 47%, 27% and 18%, respectively. ID and IDA prevalence was low and did not significantly decrease over time. Regarding gut microbiota, <i>E</i><i>nterobacteriaceae</i> decreased over time without differences between groups, whereas <i>Bifidobacteriaceae</i> and pathogenic <i>E. coli</i> were not affected. In conclusion, the multi-nutrient fortified dairy-based drink reduced anaemia in a dose-dependent way, without stimulating intestinal potential pathogenic bacteria, and thus appears to be safe and effective in treating anaemia in Nigerian toddlers.
  • Growth and micronutrient status parameters of Nigerian preterm infants consuming preterm formula or breastmilk

    Owolabi, Adedotun Joshua; Ayede, Idowu Adejumoke; Akinrinoye, Olugbenga Oyewumi; Falade, Adegoke Gbadegesin; Ajibola, Gboyega Bosun; Christopher, Ologunore Olufisayo; Arifalo, Gregory Olawole; Abiona, Ayodele Oladejo; Feskens, Edith J. M.; Melse-Boonstra, Alida; et al. (Springer Nature, 2024-01-09)
    Background: Moderate-to-late preterm infants (32–34 weeks GA) have increased risk of neonatal morbidities compared to term infants, however dedicated nutritional guidelines are lacking. Methods: Moderate-to-late preterm infants received a preterm formula (n = 17) or breastmilk (n = 24) from age 2–10 weeks in a non-randomized, open-label observational study. Anthropometric measurements were assessed bi-weekly. Blood concentrations of hemoglobin, ferritin, serum retinol, and 25-hydroxy-vitamin D (25OHD) were analyzed at age 2 and 10 weeks. Result: Average growth per day was 14.7 g/kg BW/day in formula-fed and 12.8 g/kg BW/day in breastmilk-fed infants but not different from each other. Length and head circumference in both groups were in line with the median reference values of the Fenton growth chart. At 10 weeks of age, hemoglobin tended to be higher in the formula-fed group (10.2 g/dL vs. 9.6 g/dL, p = 0.053). 25OHD increased in formula- and breastmilk-fed infants from 73.8 to 180.9 nmol/L and from 70.7 to 97.6 nmol/L, respectively. Serum retinol only increased in the formula-fed group (0.63 to 1.02 µmol/L, p < 0.001). Conclusion: Breastfeeding resulted in adequate growth in moderate-late preterm infants but was limiting in some micronutrients. The preterm formula provided adequate micronutrients, but weight gain velocity was higher than the Fenton reference value. Impact statement: Unfortified breastmilk resulted in adequate growth in weight, length and head circumference in Nigerian moderate to late preterm infants during an study period of 8 weeks, but status of vitamin D, vitamin A and iron needs to be monitored. The high-energy formula, developed for very preterm infants, resulted in higher growth in body weight in moderate to late preterm infants than the median of the Fenton preterm growth chart. This study supports the necessity of dedicated nutritional guidelines, and regular monitoring of growth and nutritional status of moderate to late preterm infants.
  • Association between sugar-sweetened beverage consumption and constructs of the Health Belief Model in young adult students at the University of Chester

    Ndubuisi, Goodluck Chiemela; Fallows, Stephen; Michael Okpara University of Agriculture; University of Chester (World Public Health Nutrition Association, 2024-09-30)
    Background: Young adults are reported as one of the major consumers of sugar-sweetened beverages (SSBs) globally and the consumption of SSBs is associated with long-term medical conditions. The Health Belief Model (HBM) has been applied to understand SSB consumption by children and adolescents through the use of its constructs. There is a gap in knowlege of HBM application to SSB consumption of young adults. Therefore, the present study was undertaken to fill this gap. Objective: The primary objective of the study was to determine if an association existed between SSB consumption status and the constructs of the HBM: perceived susceptibility, perceived severity, perceived barriers, perceived benefits, self-efficacy, and cues in young adult students. Design: Cross-sectional data were obtained using an online self-administered structured questionnaire. Descriptive statistics and Chi-squared (X2) test for association were used to analyse the data. Setting: Participants were recruited via email and WhatsApp. Participants: Seventy young adult students aged 18 to 30 years studying at the University of Chester, England. Results: The mean age of the participants was 25.5 years (SD: 3.0). 53% consumed SSBs on a given day. Postgraduates (58%), Blacks (52%), and Asian students (70%) had the greater percentages of SSB consumers. There was a very strong significant association between SSB consumption status and level of perceived severity of diseases from a high intake of SSBs, X2 (1, N = 70) = 6.94, P = 0.01, Cramer’s V = 0.32. Also, a very strong association existed between SSB consumption status and self-efficacy level to control SSB intake, X2 (1, N = 70) = 8.83, P = 0.00, Cramer’s V = 0.36. Conclusions: A high percentage of young adult students especially those from minority ethnic groups in the UK consumed SSBs which indicates that targeted initiatives at these groups are required to control their intake. Interventions to control SSB intake in young adult students should consider increasing their level of perceived severity of diseases from a high intake of SSBs and self-efficacy to control SSB intake. We recommend actions that can further increase their awareness of how serious diseases from a high intake of SSBs are. We also recommend measures intended to increase the confidence of young adult students in their ability to avoid SSBs when they are stressed, after writing exam, when they are eating, and when they engage in sedentary activities. In particular, modifying their environment to prevent the availability of SSBs at home and regulating the promotion and cost (via taxation) of SSBs in stores would help to increase their self- efficacy level. Further studies to determine why a greater percentage of postgraduate students are consuming SSBs despite their level of education is required. More comprehensive research on SSB consumption in young adults using the HBM is necessary.
  • Analytical data of Acacia nilotica var. Nilotica gum

    Satti, Amira A. E.; Osman, Mohammed E.; Hassan, Elfatih A.; Al-Assaf, Saphwan; Jouf University; Sudan University of Science and Technology; University of Chester (Chemical Society of Ethiopia, 2024-04-30)
    This study aimed to characterize the exudate gum from Acacia nilotica var. nilotica in Sudan and compare its physicochemical properties to Acacia seyal var. seyal and Acacia senegal var. senegal (gum Arabic). Samples were collected from six different states in Sudan over three seasons. The gum had a moisture content of 10.50%, ash content of 1.86%, pH value of 5.19, specific optical rotation of +94.70, intrinsic viscosity of 10.44 cm3 g-1, nitrogen content of 0.024%, protein content of 0.16%, acid equivalent weight of 1907.82, and total uronic acid content of 10.18%. Sugar content analysis revealed arabinose (41.20%), galactose (17.43%), and rhamnose (10.68%). Potassium was the predominant cation, followed by calcium, magnesium, sodium, lead, and iron. Acacia nilotica was classified as part of the Gummeferae series and exhibited a positive specific optical rotation. The Number average molecular weight (Mn) was estimated using osmometric measurements and gel permeation chromatography. The gum had a higher molecular weight and lower intrinsic viscosity compared to gum Arabic, suggesting a spheroidal shape of molecule. Amino acid analysis showed similarities with gum Arabic, with hydroxyproline and serine as principal amino acids. Variations in cationic composition were attributed to differences in soil type among collection locations.
  • Using Photovoice to explore adults' perceptions towards obesity and factors influencing food choice and physical activity in North Lebanon

    Al Kattan, Malika; Fallows, Stephen; Kennedy, Lynne; University of Westminster; University of Chester; Qatar University (Cambridge University Press, 2025-10-13)
    OBJECTIVE: To explore adults' perceptions towards obesity and factors influencing eating behaviour and physical activity in North Lebanon, using a variation of the Photovoice method. DESIGN: This research is part of a broader qualitative study exploring factors influencing the rising levels of obesity and understanding the barriers and enablers for effective policy for obesity prevention using a socio-ecological model as a guiding framework. For this study, a variation of "Photovoice" was used to collect photographs to explore participants' perspectives of obesity and its causes in Lebanon, using these photographs to generate discussion in one-to-one face-to-face interviews. Inductive and deductive thematic analyses were used to analyse the transcribed interviews. SETTING: Tripoli, North Governorate, Lebanon. PARTICIPANTS: Twenty Lebanese adults aged 20-64 years were recruited. RESULTS: The participants (n=20) generated 257 photographs representing various factors perceived to be associated with rising obesity in Lebanon: changes in the food and eating landscape, sedentary behaviours, food environments, eating out, and food marketing on social media platforms. Several themes specific to Lebanon were also identified, including the perceptions towards obesity, the central role of women in Lebanese food preparation and the family, and the sociocultural importance of food and social gatherings. CONCLUSION: This study highlights how influences across the five levels of the socio-ecological model shaped the participants' food choices and physical activity levels. Collaborative initiatives and public policies are necessary to address the identified barriers and curb the increasing prevalence of obesity in Lebanon.
  • Effects of transglutaminase and epigallocatechin gallate on the structural and physicochemical properties of fish skin gelatin from Takifugu rubripes

    Han, Lingyu; Zhang, Yulong; Hu, Bing; Zhang, Ying; Cao, Jijuan; Yang, Jixin; Al-Assaf, Saphwan; Dalian Minzu University; Wrexham University; University of Chester (MDPI, 2025-09-11)
    Fish skin gelatin (FG) has garnered considerable attention as a potential substitute for mammalian gelatin. In this study, Takifugu rubripes skin gelatin was chemically modified using transglutaminase (TG) and epigallocatechin gallate (EGCG). Subsequently, the rheological, structural, and physicochemical properties of FG modified with varying concentrations of TG and EGCG were systematically examined and compared. As the concentrations of TG and EGCG increased, more extensive interactions occurred in FG, leading to a significant enhancement of gelatin properties. Following modification, the molecular weight of FG proteins increased, and this was accompanied by enhanced surface hydrophobicity and gel strength. Rheological analysis further demonstrated that the viscosity of FG modified with TG and EGCG was higher than that of unmodified FG and was positively correlated with the treatment concentrations of TG and EGCG. Additionally, the results indicated that the effect of TG modification was more pronounced than that of EGCG modification. Overall, this study demonstrates that both TG and EGCG modifications can effectively overcome the inherent limitations of fish skin gelatin, with TG showing superior efficiency as a cross-linking agent. The enhanced thermal stability, gel strength, and rheological properties achieved through these interactions significantly expand the potential applications of fish gelatin in the food industry, making it a more viable alternative to mammalian gelatin.
  • Stakeholders' perspectives on adolescent obesity prevention amid the nutrition transition in Lebanon

    Bou Kheir, Miriam; Fallows, Stephen; Kennedy, Lynne; Lebanese University; University of Chester; Zayed University (Center for Ethnic and Cultural Studies, 2025-06-23)
    Adolescent obesity has become a major public health concern globally, particularly in low- and middle-income countries undergoing rapid Nutrition Transitions. Lebanon, a middle-income country in the Eastern Mediterranean Region, has experienced substantial shifts in dietary patterns and physical activity levels in recent decades, contributing to an alarming rise in obesity among youth. This qualitative study aimed to explore the perceptions and experiences of key stakeholders in Lebanon regarding the multilevel determinants of adolescent obesity, as well as the challenges and opportunities for effective prevention strategies in this context. The study explored stakeholders' views on the drivers of adolescent obesity in Lebanon at the individual, interpersonal, community, organizational, and policy levels. The findings underscore the critical need for comprehensive, multilevel, multistakeholder approaches to address the root causes of adolescent obesity in Lebanon. This study provides timely and contextually relevant insights that can inform the development of effective and sustainable strategies to prevent and control adolescent obesity in Lebanon. It also contributes to the growing literature on the challenges and opportunities for tackling the global burden of obesity in the context of rapid Nutrition Transitions in low- and middle-income countries.
  • SCCS Opinion on Biphenyl-2-ol and Sodium 2-biphenylolate used in cosmetic products (CAS/EC No. 90-43-7/201-993-5 and 132-27-4/205-055-6)– SCCS/1669/24

    Bernauer, Ulrike; Bodin, Laurent; Chaudhry, Qasim; Coenraads, Pieter Jan; Ezendam, Janine; Gaffet, Eric; Galli, Corrado L.; Panteri, Eirini; Rogiers, Vera; Rousselle, Christophe; et al. (Elsevier, 2025-06-04)
    ‘Biphenyl-2-ol’ and ‘Sodium 2-biphenylolate’ (CAS/EC No. 90-43-7/201-993-5 and 132-27-4/205-055-6) are known with the INCI names ‘o-Phenylphenol’ and Sodium ‘o-Phenylphenate’, respectively, as cosmetic ingredients. The use of o-Phenylphenol as a preservative is currently authorised in entry 7 of Annex V to the Cosmetics Regulation (EC) No.1223/2009, with a maximum concentration of 0.15 % (as phenol) in leave-on and 0.2 % (as phenol) in rinse-off cosmetic products. The Scientific Committee on Consumer Safety (SCCS) adopted an opinion on o-Phenylphenol (OPP), Sodium o-Phenylphenate (SOPP), and Potassium o-Phenylphenate (POPP) (SCCS/1555/15) in June 2015, later revised on 15 December 2015, with the following conclusion: ‘Based on the information provided, no conclusions of safe use can be drawn for Sodium o-Phenylphenate and Potassium o-Phenylphenate’. In 2018, the SCCS released an addendum to the above scientific opinion, specifically addressing Sodium o-Phenylphenate, Potassium o-Phenylphenate, and MEA o-Phenylphenate. The SCCS concluded that ‘Due to the lack of relevant information, the SCCS is unable to answer the question regarding the safe use level of sodium-OPP, potassium-OPP, and MEA-OPP. In the SCCS's view, a direct comparison between the safety of o-Phenylphenate (OPP) and its three compounds cannot be made’. The conclusions of this opinion resulted in amending entry 7 of Annex V to the Cosmetics Regulation, removing from the list the previously authorised OPP salts. It should be noted that o-Phenylphenol, Sodium and Potassium o-Phenylphenate are active ingredients in broad-spectrum fungicides surface biocides. Under EU biocidal Regulation (EU) 528/2012, o-Phenylphenol has been evaluated for the different product types (PTs) such as PT 1, PT 2, PT4, PT 6 as a preservative ranging from 0.1 to 0.5 % w/w. The European Risk Assessment Committee (RAC) of ECHA issued in December 2022 an opinion recommending among others a classification for o-Phenylphenol as ‘Carcinogen of Category 2’. Following the RAC opinion, the European Commission may propose a classification for o-Phenylphenol as a ‘Carc.2’ (CLP Regulation Annex VI entry). According to Article 15(1) of the Cosmetics Regulation ‘the use in cosmetic products of substances classified as CMR substances, of category 2, under Part 3 of Annex VI to Regulation (EC) No 1272/2008 shall be prohibited. However, a substance classified in category 2 may be used in cosmetic products where the substance has been evaluated by the SCCS and found safe for use in cosmetic products’. In view of these provisions, regulatory measures must be adopted by the Commission services within 15 months of the classification as CMR 1A or 1B of the substance(s) concerned in Part 3 of Annex VI to Regulation (EC) No 1272/2008. In December 2023, the Commission services received a dossier to defend the safe use of o-Phenylphenol, as well as its sodium salt (CAS/EC No. 90-43-7/201-993-5 and 132-27-4/205-055-6) as preservatives in cosmetic products according to Article 15(1) of the Cosmetics Regulation 1223/2009. The Commission, therefore, requests the SCCS to carry out a safety assessment on these ingredients in view of the information provided.
  • An Investigation of Hydrocolloids Film Forming Ability on a Vertically Rotating Disc

    Miah, Salim; McMillan, Alison; Al-Assaf, Saphwan; University of Hertfordshire; Wrexham University; University of Chester (Bangladesh Academy of Sciences, 2023-12-30)
    Hydrocolloids are widely used in the food industry to perform variety of functions such as coatings, thickening, emulsifying, stabilizing and edible films. Their functionality for a given application are underpinned by the molecular weight, shape, and conformation in aqueous solution. The film forming ability of selected hydrocolloids, different in shape (rod, random coil and spherical) and/or conformation in aqueous solution were investigated experimentally and numerically on a vertically rotating disc. These include: xanthan, pectin, carboxymethyl cellulose (CMC) and gum arabic. The Laser scan method was used for the measurement of film thickness of the respective the hydrocolloids. The Volume of Fluid (VOF) Computational Fluid Dynamics (CFD) modelling approach was used in the numerical model. The variation in film formation at different concentrations has been observed to ascertain a trend. Both the experimental and simulation results revealed that the film formation depends on the molecular structure of the hydrocolloid while viscosity and rotating speed significantly influenced the film thickness. Xanthan showed higher film formation ability compared to the other hydrocolloids due to its higher viscosity. It was interesting to note that the film formation ability by CMC was significantly higher than pectin though pectin was five times more viscous than CMC. Gum arabic exhibited the lowest viscosity but formed almost the same film thickness on the disc as pectin despite being twenty times less viscous. Increasing CMC concentration from 0.5% to 1% resulted in increasing its viscosity and the film thickness. The film thickness increased at the disc rotating speed of 6 rpm as compared to 3 rpm. The simulation results were in good agreement with the experimental data.
  • UPLC-Q-TOF-MS/MS identification, antioxidant, and alpha-glucosidase inhibition activities of three forms of phenolic compounds from Yemeni jujube fruit: In vitro and in silico investigations

    Aleryani, Hamzah; Abdo, Abdullah A. A.; Al-Assaf, Saphwan; Al-Zamani, Zakarya; Auriol Ivane, Ngoua Moffo; Guifang, Tian; Al-Romaima, Abdulbaset; Tan, Jianxin; Sang, Yaxin; Hebei Agricultural University; University of Chester; Ibb University; Chinese Academy of Sciences; Iowa State University (Elsevier, 2025-03-12)
    Jujube fruits have rich medicinal value due to their biological properties which are affected mainly by geographical locations. This study investigates the antioxidant properties of free fraction (FF), esterified fraction (EF), bound fraction (BF), and other metabolites prepared from dried Yemeni jujube fruit and compare to those previously reported in the literature. Results from UPLC-Q-TOF-MS/MS analysis showed that this fruit consists of a total of 33, 26, and 27 free, esterified, and bound compounds identified, respectively. In terms of ABTS, DPPH, FRAP, and H<sub>2</sub>O<sub>2</sub> assays, the BF exhibited values 1.2, 1.3,1.3, and 1.4 times higher than those of the FF, and similarly, 1.8, 1.9,2.0, and 2.5 times higher than the EF. A similar trend was observed in the inhibition of α-glucosidase (α-Glu), where the BF was 1.6, and 2.4 times more effective compared to the FF and EF, respectively. In silico analysis showed that flavonoids, the key active compounds, had substantial α-glucosidase binding capacity. In the meat model, all fractions inhibited protein and lipid oxidation during storage, and the BF exhibited the highest antioxidant capacity. This study provides the first investigation of the structure of active compounds and their biological properties in Yemeni jujube fruit. Yemeni fruits can be utilized as natural antioxidants, and nutraceuticals in food preservation, and medicine respectively.
  • A 12-week double-blind randomised controlled trial investigating the effect of dietary supplementation with 125 µg/d vitamin D in adults with asthma – ERRATUM

    Watkins, Stephanie; Harrison, Tanja; Mushtaq, Sohail; University of Chester (Cambridge University Press, 2024-09-28)
    Erratum
  • Analytical study on secondary metabolites extraction and characterization from Acacia Gums (AGs)

    Elnour, Ahmed A. M.; Al-Assaf, Saphwan; Kabbashi, Nassereldeen Ahmed; Osman, Mohammed Elmubark; Abdelgadir, Abdelgadir Elfadil; Universiti Malaysia Pahang Al-Sultan Abdullah; University Malaysia; University of Chester; International Islamic University; Sudan University of Science and Technology; Al-Neelain University (Springer, 2025-02-11)
    Acacia gums (AGs), derived from Acacia senegal, Acacia seyal, and Acacia polyacantha trees, are widely used in various industries, including food, pharmaceuticals, and cosmetics. They serve as emulsifiers, stabilizers, thickeners, and soluble dietary fibers. This study aimed to optimize the extraction process of secondary metabolites extracted (SME) from AGS to enhance their efficiency and quality for industrial applications. Gum arabic, primarily composed of D-galactose, L-arabinose, L-rhamnose, D-glucuronic acid, and 4-O-methyl-D-glucuronic acid with a small fraction of proteins, is a multifunctional ingredient known for its emulsifying activity, attributed to its amphiphilic nature. Its effectiveness as an emulsifier stems from its high water solubility, low-viscosity, good surface activity, and ability to form a protective film around emulsion droplets. AGS in Sudan play crucial ecological roles by providing fodder, firewood, gums, and soil erosion control, which are essential for gum arabic production. Sudan’s diverse biodiversity hosts many plant species traditionally used for medicinal purposes, with extensive knowledge of medicinal plant species stemming from their widespread use among different ethnic groups. This study highlights the importance of optimizing the extraction of secondary metabolites from AGS for industrial applications, and the significance of AGS in Sudan’s ecological balance and economic stability.
  • Isolation and characterisation of acid soluble collagens and pepsin soluble collagens from Eel (Anguilla japonica Temminck et Schlegel) skin and bone

    Zhang, Ningning; Guo, Shaoli; Zheng, Yuting; Li, Weili; University of Chester; Fujian Agriculture and Forestry University (MDPI, 2025-02-05)
    Eel (Anguilla japonica) is an important and valuable food fish in East Asia and its by-products have been reported to include bioactive and profitable components. This study aimed to extract, characterise, and compare the structure and properties of acid-soluble collagens (ASCs) and pepsin-soluble collagens (PSCs) from the skin and bone of eel (Anguilla japonica), providing insights into their composition, structure, and properties for various applications. The yields of ASC-S (from skin), PSC-S (from skin), ASC-B (from bone), and PSC-B (from bone) were 12.16%, 15.54%, 0.79%, and 1.34% on a dry weight basis, respectively. Glycine, the dominant amino acid, accounted for 16.66% to 22.67% of total amino acids in all samples. SDS-PAGE and FTIR analyses showed the typical triple-helical structure of type I collagen with slight variations in molecular order in extract and intermolecular cross-linking between skin and bone collagens. The denaturation temperature (Tmax1) measured by differential scanning calorimetry (DSC) is 81.39 °C and 74.34 °C, respectively, for ASC-B and ASC-S. Bone collagen has higher thermal resistance than skin collagen. Surface morphology imaged using a scanning electron microscope (SEM) showed that the bone collagen had a denser network structure, whilst the skin collagen was more fibrous and porous. The findings suggest that eel-derived collagens from skin and bone can serve as potential alternatives in the food, cosmetic, and healthcare industries.
  • “Some people sit, some people stand, that’s just what we do ”: A qualitative exploration of sit-stand desk use in naturalistic settings

    Deery, Elizabeth; Buckley, John; Morris, Michael; Kennedy, Lynne; Ulster University; Keele University; University of Chester; Qatar University (Springer, 2024-03-11)
    Prolonged sedentary behaviour is associated with poor health. Office-based workers spend much of the working day sitting. Sit-stand desks have become a popular intervention, but real-life experiences of the value of sit-stand desks remain relatively unexplored outside of research-led interventions. A qualitative study was therefore undertaken to explore real-life experiences, including perceived barriers, and facilitators of using sit-stand desks. Six focus-group interviews were undertaken with a total of 34 desk-based employees in North-West England. Interviews were transcribed and analysed using thematic analysis. Three main themes emerged: The Sedentary Office, Motivators, and The Active Office. The Sedentary Office highlighted participants’ frustration with an increasingly sedentary office environment. Motivators to reducing sitting time were knowledge of the health consequences of prolonged sitting, previous (ill) health, and the potential benefits expected. The Active Office highlighted the importance of control, choice, enhanced interaction with colleagues, and the benefits associated with moving more and enhancing an overall sense of workplace wellbeing. Perceived benefits, facilitators, and barriers - experienced and observed – from sit-stand desk use in the real world were examined, thus contributing to important discussions of transferability. Overall, the study shed light on the perceived facilitators, benefits, and drawbacks of sit-stand desk work whilst also giving evidence of the real-world acceptability and research translation of sit-stand desk use. Future research should examine this in multiple contexts and should explore implications of an increasing prevalence in home working.
  • Exploring puffed rice as a novel Ink for 3D food printing: Rheological characterization and printability analysis

    Park, Bo-Ram; No, Junhee; Oh, Hyeonbin; Park, Chan-Soon; You, Kwan-Mo; Chewaka, Legesse S.; National Institute of Agricultural Science, Korea; Kyungpook National University; University of Chester (Elsevier, 2024-09-07)
    This study introduces a novel approach by using puffed rice (PR) as a sustainable and innovative ink for 3D food printing. Due to gelatinization and dextrinization, PR saw notable water absorption and solubility gains, with a modest viscosity uptick from 39.2 to 49.9 RVU, sharply contrasting Native rice (NR)'s jump from 128.9 to 167.8 RVU, emphasizing PR's minimal retrogradation. Gelatinized rice (GR) demonstrates similar stability in viscosity changes as PR, yet it requires more water and extended processing times for gelatinization. Conversely, PR's puffing process, which eliminates the need for water, offers quicker preparation and notable environmental benefits. Rheological analysis at 25% PR concentration reveals an optimal balance of viscosity (η, 897.4 Pa s), yield stress (τy, 2471.3 Pa), and flow stress (τf, 1509.2 Pa), demonstrating superior viscoelastic properties that facilitate enhanced printability and shape fidelity. Texture Profile Analysis outcomes reveals that PR significantly enhances key textural properties including hardness, adhesiveness, and springiness at this specific concentration. These findings highlight PR's potential as an eco-friendly and efficient ink choice for 3D-printed food products, providing enhanced performance and sustainability compared to GR and NR.
  • A multi-dimensional Sustainable Diet Index (SDI) for Ghanaian adults under transition: The RODAM study

    Okekunle, Akinkunmi P.; Nicolaou, Mary; De Allegri, Manuela; Meeks, Karlijn A. C.; Osei-Kwasi, Hibbah; Stockemer, Julia; de-Graft Aikins, Anna; Agbemafle, Isaac; Bahendeka, Silver; Boateng, Daniel; et al. (BioMed Central, 2024-10-01)
    The sustainability of diets consumed by African populations under socio-economic transition remains to be determined. This study developed and characterized a multi-dimensional Sustainable Diet Index (SDI) reflecting healthfulness, climate-friendliness, sociocultural benefits, and financial affordability using individual-level data of adults in rural and urban Ghana and Ghanaian migrants in Europe to identify the role of living environment in dietary sustainability. Methods: We used cross-sectional data from the multi-centre Research on Obesity and Diabetes among African Migrants Study (N = 3169; age range: 25–70 years). For the SDI construct (0–16 score points), we used the Diet Quality Index-International, food-related greenhouse gas emission, the ratio of natural to processed foods, and the proportion of food expenditure from income. In linear regression analyses, we estimated the adjusted ß-coefficients and 95% confidence intervals (CIs) for the differences in mean SDI across study sites (using rural Ghana as a reference), accounting for sociodemographic and lifestyle factors. Results: The overall mean SDI was 8.0 (95% CI: 7.9, 8.1). Participants in the highest SDI-quintile compared to lower quintiles were older, more often women, non-smokers, and alcohol abstainers. The highest mean SDI was seen in London (9.1; 95% CI: 8.9, 9.3), followed by rural Ghana (8.2; 95% CI: 8.0, 8.3), Amsterdam (7.9; 95% CI: 7.7, 8.1), Berlin (7.8; 95% CI: 7.6, 8.0), and urban Ghana (7.7; 95% CI: 7.5, 7.8). Compared to rural Ghana, the differences between study sites were attenuated after accounting for age, gender and energy intake. No further changes were observed after adjustment for lifestyle factors. Conclusion: The multi-dimensional SDI describes four dimensions of dietary sustainability in this Ghanaian population. Our findings suggest that living in Europe improved dietary sustainability, but the opposite seems true for urbanization in Ghana.

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