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dc.contributor.authorElla-Tongwiis, Peter
dc.contributor.authorLamb, Rebecca M.
dc.contributor.authorMakanga, Alexander
dc.contributor.authorShergill, Iqbal
dc.contributor.authorHughes, Stephen F.
dc.date.accessioned2024-10-28T16:21:58Z
dc.date.available2024-10-28T16:21:58Z
dc.date.issued2020-11-25
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/629098/Lamb%20-%20Antibody%20expression.pdf?sequence=1
dc.identifier.citationElla-Tongwiis, P., Lamb, R. M., Makanga, A., Shergill, I., & Hughes, S. F. (2020). The role of antibody expression and their association with bladder cancer recurrence: a single-centre prospective clinical-pilot study in 35 patients. BMC Urology, 20, article-number 187. https://doi.org/10.1186/s12894-020-00759-3en_US
dc.identifier.doi10.1186/s12894-020-00759-3en_US
dc.identifier.urihttp://hdl.handle.net/10034/629098
dc.description.abstractBladder cancer (BC) is the 10th most common cancer in the UK, with about 10,000 new cases annually. About 75–85% of BC are non-muscle invasive (NMIBC), which is associated with high recurrence and progression rates (50–60% within 7–10 years). There are no routine biomarkers currently available for identifying BC patients at increased risk of developing recurrence. The focus of this research study was to evaluate antibody expression in BC patients and their association with cancer recurrence. Methods: 35 patients scheduled for TURBT were recruited after written informed consent. Ethical approval for the project was granted via IRAS (REC4: 14/WA/0033). Following surgical procedure, tissues were preserved in 10% buffered formalin and processed within 24 h in FFPE blocks. 7 sections (4 µm each) were cut from each block and stained for CD31, Human epidermal growth factor receptor-2 (HER-2), S100P, Cyclooxygenase-2 (COX-2), VEGFR-3 thrombomodulin and CEACAM-1 using immunohistochemistry. Clinical outcome measures (obtained via cystoscopy) were monitored for up to 6 months following surgical procedure. Results: There was significantly increased expression of CD31 (p < 0.001), HER-2 (p = 0.032), S100P (p < 0.001), COX-2 (p < 0.001), VEGFR-3 (p < 0.001) and decreased expression of thrombomodulin (p = 0.010) and CEACAM-1 (p < 0.001) in bladder tumours compared to normal bladder tissues. HER-2 expression was also significantly associated with cancer grade (p = 0.003), especially between grade 1 and grade 2 (p = 0.002) and between grade 1 and grade 3 (p = 0.004). There was also a significant association between cancer stage and HER-2 expression (p < 0.001). Although recurrence was significantly associated with cancer grade, there was no association with antibody expression. Conclusion: Findings from the present study may indicate an alternative approach in the monitoring and management of patients with BC. It is proposed that by allowing urological surgeons access to laboratory markers such as HER-2, Thrombomodulin and CD31 (biomarker profile), potentially, in the future, these biomarkers may be used in addition to, or in combination with, currently used scoring systems to predict cancer recurrence. However, verification and validation of these biomarkers are needed using larger cohorts.en_US
dc.description.sponsorshipBCUHB Department of Research & Innovationen_US
dc.publisherBMCen_US
dc.relation.urlhttps://bmcurol.biomedcentral.com/articles/10.1186/s12894-020-00759-3en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectAntibody expressionen_US
dc.subjectBladder canceren_US
dc.titleThe Role of Antibody Expression and Their Association With Bladder Cancer Recurrence: A Single-Centre Prospective Clinical-Pilot Study in 35 Patientsen_US
dc.typeArticleen_US
dc.identifier.eissn1471-2490en_US
dc.contributor.departmentWrexham Maelor Hospital; University of Chester; Betsi Cadwaladr University Health Board; Wrexham Glyndwr Universityen_US
dc.identifier.journalBMC Urologyen_US
dc.date.accepted2020-11-18
rioxxterms.identifier.projectn/aen_US
rioxxterms.versionVoRen_US
dc.date.deposited2024-10-28en_US


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