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dc.contributor.authorSebaiy, Mahmoud M.
dc.contributor.authorZiedan, Noha
dc.date.accessioned2023-02-09T12:40:25Z
dc.date.available2023-02-09T12:40:25Z
dc.date.issued2019-11-24
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/627541/CDM%20FULL%20LOR%20DES%20manuscript%20_%20%28002%29.pdf?sequence=3
dc.identifier.citationSebaiy, M. & Ziedan, N. (2019). Developing A High-performance Liquid Chromatography Method for Simultaneous Determination of Loratadine and its Metabolite Desloratadine in Human Plasma. Current Drug Metabolism, 20(13), 1053-1059. http://dx.doi.org/10.2174/1389200220666191125095648en_US
dc.identifier.issn1389-2002
dc.identifier.doi10.2174/1389200220666191125095648
dc.identifier.urihttp://hdl.handle.net/10034/627541
dc.description.abstractBackground: Allergic diseases are considered as the major burden on public health with increased prevalence globally. Histamine H1-receptor antagonists are the foremost commonly used drugs in the treatment of allergic disorders. The target drug in this study, loratadine, belongs to this class of drugs and its biometabolite desloratadine which is also a non-sedating H1 receptor antagonist with anti-histaminic activity being 2.5 to 4 times greater than loratadine. This study aimed to develop and validate a novel isocratic Reversed-phase High-Performance Liquid Chromatography (RP-HPLC) method for rapid and simultaneous separation and determination of loratadine and its metabolite, desloratadine in human plasma. Methods: The drug extraction method from plasma was based on protein precipitation technique. The separation was carried out on a Thermo Scientific BDS Hypersil C18 column (5μm, 250 x 4.60 mm) in a mobile phase of MeOH: 0.025M KH2PO4 adjusted to pH 3.50 using orthophosphoric acid (85: 15, v/v) at an ambient temperature. The flow rate was maintained at 1 mL/min and maximum absorption was measured using the PDA detector at 248 nm. Results: The retention times of loratadine and desloratadine in plasma samples were recorded to be 4.10 and 5.08 minutes, respectively, indicating a short analysis time. Limits of detection were found to be 1.80 and 1.97 ng/mL for loratadine and desloratadine, respectively, showing a high degree of sensitivity of the method. The method was then validated according to FDA guidelines for the determination of the two analytes in human plasma. Conclusion: The results obtained indicate that the proposed method is rapid, sensitive in the nanogram range, accurate, selective, robust and reproducible compared to other reported methods.en_US
dc.publisherBentham Scienceen_US
dc.relation.urlhttp://www.eurekaselect.com/176942/articleen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectHPLCen_US
dc.subjectSimultaneous separationen_US
dc.subjectHuman plasmaen_US
dc.titleDeveloping A High-performance Liquid Chromatography Method for Simultaneous Determination of Loratadine and its Metabolite Desloratadine in Human Plasmaen_US
dc.typeArticleen_US
dc.identifier.eissn1875-5453en_US
dc.contributor.departmentUniversity of Chesteren_US
dc.identifier.journalCurrent Drug Metabolismen_US
or.grant.openaccessYesen_US
rioxxterms.funderunfundeden_US
rioxxterms.identifier.projectunfundeden_US
rioxxterms.versionAMen_US
rioxxterms.versionofrecord10.2174/1389200220666191125095648en_US
rioxxterms.licenseref.startdate2019-11-24
dcterms.dateAccepted2019-11-20
rioxxterms.publicationdate2019-11-24
dc.dateAccepted2019-09-05
dc.date.deposited2023-02-09en_US
dc.indentifier.issn1389-2002en_US


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