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dc.contributor.authorGiakoumaki, Ifigeneia
dc.contributor.authorPollock, Natalie
dc.contributor.authorAljuaid, Turki
dc.contributor.authorSannicandro, Anthony J.
dc.contributor.authorAlameddine, Moussira
dc.contributor.authorOwen, Euan
dc.contributor.authorMyrtziou, Ioanna
dc.contributor.authorOzanne, Susan E.
dc.contributor.authorKanakis, Ioannis
dc.contributor.authorGoljanek-Whysall, Katarzyna
dc.contributor.authorVasilaki, Aphrodite
dc.date.accessioned2022-09-05T10:16:41Z
dc.date.available2022-09-05T10:16:41Z
dc.date.issued2022-08-08
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/627148/ijms-23-08815-v2.pdf?sequence=1
dc.identifier.citationGiakoumaki, I., Pollock, N., Aljuaid, T., Sannicandro, A. J., Alameddine, M., Owen, E., Myrtziou, I., Ozanne, S. E., Kanakis, I., Goljanek-Whysall, K., & Vasilaki, A. (2022). Postnatal protein intake as a determinant of skeletal muscle structure and function in mice - A pilot study. International Journal of Molecular Sciences, 23(15), 8815. https://doi.org/10.3390/ijms23158815en_US
dc.identifier.issn1661-6596
dc.identifier.doi10.3390/ijms23158815
dc.identifier.urihttp://hdl.handle.net/10034/627148
dc.description.abstractSarcopenia is characterised by an age-related decrease in the number of muscle fibres and additional weakening of the remaining fibres, resulting in a reduction in muscle mass and function. Many studies associate poor maternal nutrition during gestation and/or lactation with altered skeletal muscle homeostasis in the offspring and the development of sarcopenia. The aim of this study was to determine whether the musculoskeletal physiology in offspring born to mouse dams fed a low-protein diet during pregnancy was altered and whether any physiological changes could be modulated by the nutritional protein content in early postnatal stages. Thy1-YFP female mice were fed ad libitum on either a normal (20%) or a low-protein (5%) diet. Newborn pups were cross-fostered to different lactating dams (maintained on a 20% or 5% diet) to generate three groups analysed at weaning (21 days): Normal-to-Normal (NN), Normal-to-Low (NL) and Low-to-Normal (LN). Further offspring were maintained ad libitum on the same diet as during lactation until 12 weeks of age, creating another three groups (NNN, NLL, LNN). Mice on a low protein diet postnatally (NL, NLL) exhibited a significant reduction in body and muscle weight persisting up to 12 weeks, unlike mice on a low protein diet only prenatally (LN, LNN). Muscle fibre size was reduced in mice from the NL but not LN group, showing recovery at 12 weeks of age. Muscle force was reduced in NLL mice, concomitant with changes in the NMJ site and changes in atrophy-related and myosin genes. In addition, μCT scans of mouse tibiae at 12 weeks of age revealed changes in bone mass and morphology, resulting in a higher bone mass in the NLL group than the control NNN group. Finally, changes in the expression of miR-133 in the muscle of NLL mice suggest a regulatory role for this microRNA in muscle development in response to postnatal diet changes. Overall, this data shows that a low maternal protein diet and early postnatal life low-protein intake in mice can impact skeletal muscle physiology and function in early life while postnatal low protein diet favours bone integrity in adulthood.en_US
dc.publisherMDPIen_US
dc.relation.urlhttps://www.mdpi.com/1422-0067/23/15/8815en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectMiceen_US
dc.subjectMaternal protein restrictionen_US
dc.subjectSkeletal muscleen_US
dc.subjectNeuromuscular junctionen_US
dc.subjectOffspringen_US
dc.subjectmicroRNAsen_US
dc.titlePostnatal Protein Intake as a Determinant of Skeletal Muscle Structure and Function in Mice-A Pilot Studyen_US
dc.typeArticleen_US
dc.identifier.eissn1422-0067en_US
dc.contributor.departmentUniversity of Liverpool; NUI Galway; Taif University; University of Chester; University of Cambridgeen_US
dc.identifier.journalInternational Journal of Molecular Sciencesen_US
or.grant.openaccessYesen_US
rioxxterms.funderDepartmental Research Support Budget, University of Liverpoolen_US
rioxxterms.identifier.projectJXR12557en_US
rioxxterms.versionVoRen_US
rioxxterms.versionofrecord10.3390/ijms23158815en_US
dcterms.dateAccepted2022-07-27
rioxxterms.publicationdate2022-08-08
dc.date.deposited2022-09-05en_US
dc.indentifier.issn1661-6596en_US


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