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dc.contributor.authorRandles, Michael
dc.contributor.authorLennon, Rachel
dc.date.accessioned2022-08-26T08:37:44Z
dc.date.available2022-08-26T08:37:44Z
dc.date.issued2015-11-23
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/627113/1-s2.0-S1063582315000587-main.pdf?sequence=1
dc.identifier.citationRandles, M., & Lennon, R. (2015). Applying proteomics to investigate extracellular matrix in health and disease. Basement membranes, (pp. 171-196). Academic Press.en_US
dc.identifier.isbn9780128040157en_US
dc.identifier.urihttp://hdl.handle.net/10034/627113
dc.description.abstractThe molecular composition of basement membranes (BMs) has traditionally been investigated by candidate-based approaches leading to the identification of key structural components as described in previous chapters. Laminins, collagen IV, nidogens, perlecan, and type XV/XVIII collagen are integral to BMs with isoforms showing tissue specificity. More recently the application of mass spectrometry (MS)-based proteomics has led to the discovery of many more structural and regulatory components of BMs and more broadly, extracellular matrix (ECM). These investigations have revealed tissue-specific signatures of between 100 and 150 ECM components, demonstrating the complexity of the extracellular niche. In addition to providing a structural scaffold for cells, ECM is a dynamic extracellular environment capable of regulating the physical properties of tissues. Global investigations of ECM with proteomics in turn enable systems level analyses and when applied to health and disease states these investigations provide insights into pathways regulating matrix dysregulation. This chapter focuses on the methods used to extract ECM and on the analysis of its composition using MS-based proteomics, and it provides examples of how these approaches have been used to investigate health and disease states.en_US
dc.publisherAcademic Pressen_US
dc.relation.urlhttps://www.elsevier.com/books/basement-membranes/miner/978-0-12-804015-7en_US
dc.relation.urlhttps://www.sciencedirect.com/bookseries/current-topics-in-membranes/vol/76/suppl/Cen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectBioinformaticsen_US
dc.subjectDegradomicsen_US
dc.subjectExtracellular matrixen_US
dc.subjectFractionationen_US
dc.subjectGlycoproteomicsen_US
dc.subjectMass spectrometryen_US
dc.subjectProteomicsen_US
dc.subjectQuantificationen_US
dc.subjectSystems biologyen_US
dc.titleChapter 5: Applying Proteomics to Investigate Extracellular Matrix in Health and Diseaseen_US
dc.typeBook chapteren_US
dc.contributor.departmentUniversity of Manchester; Manchester Academic Health Science Centreen_US
or.grant.openaccessYesen_US
rioxxterms.funderWellcomeen_US
rioxxterms.identifier.projectWellcomeen_US
rioxxterms.versionAMen_US
rioxxterms.licenseref.startdate2215-11-23
dcterms.dateAccepted2015-07-07
rioxxterms.publicationdate2015-11-23
dc.date.deposited2022-08-26en_US


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