Chapter 5: Applying Proteomics to Investigate Extracellular Matrix in Health and Disease
Name:
1-s2.0-S1063582315000587-main.pdf
Embargo:
2215-11-23
Size:
1.064Mb
Format:
PDF
Request:
Book chapter
Affiliation
University of Manchester; Manchester Academic Health Science CentrePublication Date
2015-11-23
Metadata
Show full item recordAbstract
The molecular composition of basement membranes (BMs) has traditionally been investigated by candidate-based approaches leading to the identification of key structural components as described in previous chapters. Laminins, collagen IV, nidogens, perlecan, and type XV/XVIII collagen are integral to BMs with isoforms showing tissue specificity. More recently the application of mass spectrometry (MS)-based proteomics has led to the discovery of many more structural and regulatory components of BMs and more broadly, extracellular matrix (ECM). These investigations have revealed tissue-specific signatures of between 100 and 150 ECM components, demonstrating the complexity of the extracellular niche. In addition to providing a structural scaffold for cells, ECM is a dynamic extracellular environment capable of regulating the physical properties of tissues. Global investigations of ECM with proteomics in turn enable systems level analyses and when applied to health and disease states these investigations provide insights into pathways regulating matrix dysregulation. This chapter focuses on the methods used to extract ECM and on the analysis of its composition using MS-based proteomics, and it provides examples of how these approaches have been used to investigate health and disease states.Citation
Randles, M. J., & Lennon, R. (2015). Applying proteomics to investigate extracellular matrix in health and disease. Basement membranes, (pp. 171-196). Academic Press.Publisher
Academic PressAdditional Links
https://www.elsevier.com/books/basement-membranes/miner/978-0-12-804015-7https://www.sciencedirect.com/bookseries/current-topics-in-membranes/vol/76/suppl/C
Type
Book chapterISBN
9780128040157Collections
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/