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dc.contributor.authorMohammed, Noor A.
dc.contributor.authorHakeem, Israa J.
dc.contributor.authorHodges, Nikolas
dc.contributor.authorMichelangeli, Francesco
dc.date.accessioned2022-01-24T01:49:53Z
dc.date.available2022-01-24T01:49:53Z
dc.date.issued2022-01-01
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/626659/article.pdf?sequence=2
dc.identifier.citationMohammed, N. A., Hakeem, I. J., Hodges, N., & Michelangeli, F. (2022). Regucalcin ameliorates doxorubicin-induced cytotoxicity in Cos-7 kidney cells and translocates from the nucleus to the mitochondria. Bioscience Reports, 42(1), BSR20211464. https://doi.org/10.1042/BSR20211464
dc.identifier.issn0144-8463
dc.identifier.doi10.1042/BSR20211464
dc.identifier.urihttp://hdl.handle.net/10034/626659
dc.description.abstractDoxorubicin (DOX) is a potent anticancer drug, which can have unwanted side-effects such as cardiac and kidney toxicity. A detailed investigation was undertaken of the acute cytotoxic mechanisms of DOX on kidney cells, using Cos-7 cells as kidney cell model. Cos-7 cells were exposed to DOX for a period of 24 h over a range of concentrations, and the LC50 was determined to be 7 µM. Further investigations showed that cell death was mainly via apoptosis involving Ca2+ and caspase 9, in addition to autophagy. Regucalcin (RGN), a cytoprotective protein found mainly in liver and kidney tissues, was overexpressed in Cos-7 cells and shown to protect against DOX-induced cell death. Subcellular localization studies in Cos-7 cells showed RGN to be strongly correlated with the nucleus. However, upon treatment with DOX for 4 h, which induced membrane blebbing in some cells, the localization appeared to be correlated more with the mitochondria in these cells. It is yet to be determined whether this translocation is part of the cytoprotective mechanism or a consequence of chemically induced cell stress.
dc.languageeng
dc.publisherPortland Press
dc.relation.urlhttps://portlandpress.com/bioscirep/article/42/1/BSR20211464/230443/Regucalcin-ameliorates-doxorubicin-induced
dc.rightsLicence for this article: cc by
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceissn: 0144-8463
dc.sourcenlmid: 8102797
dc.sourceessn: 1573-4935
dc.subjectApoptosis
dc.subjectcolocalization
dc.subjectnecrosis
dc.subjectCalcium Signalling
dc.subjectRegucalcin
dc.titleRegucalcin ameliorates doxorubicin-induced cytotoxicity in Cos-7 kidney cells and translocates from the nucleus to the mitochondria.
dc.typeArticle
dc.identifier.eissn1573-4935
dc.contributor.departmentUniversity of Birmingham; University of Duhok; University of Chester
dc.identifier.journalBioscience Reports
dc.date.updated2022-01-24T01:49:53Z


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