A Promiscuous Bacterial P450: The Unparalleled Diversity of BM3 in Pharmaceutical Metabolism
AuthorsThistlethwaite, Sian; email: email@example.com
Jeffreys, Laura N.; orcid: 0000-0002-0607-6116; email: Laura.Jeffreys@lstmed.ac.uk
Girvan, Hazel M.; orcid: 0000-0003-2299-8644; email: H.M.Girvan@hud.ac.uk
McLean, Kirsty J.; email: K.J.Mclean@hud.ac.uk
Munro, Andrew W.; email: firstname.lastname@example.org
MetadataShow full item record
AbstractCYP102A1 (BM3) is a catalytically self-sufficient flavocytochrome fusion protein isolated from Bacillus megaterium, which displays similar metabolic capabilities to many drug-metabolizing human P450 isoforms. BM3′s high catalytic efficiency, ease of production and malleable active site makes the enzyme a desirable tool in the production of small molecule metabolites, especially for compounds that exhibit drug-like chemical properties. The engineering of select key residues within the BM3 active site vastly expands the catalytic repertoire, generating variants which can perform a range of modifications. This provides an attractive alternative route to the production of valuable compounds that are often laborious to synthesize via traditional organic means. Extensive studies have been conducted with the aim of engineering BM3 to expand metabolite production towards a comprehensive range of drug-like compounds, with many key examples found both in the literature and in the wider industrial bioproduction setting of desirable oxy-metabolite production by both wild-type BM3 and related variants. This review covers the past and current research on the engineering of BM3 to produce drug metabolites and highlights its crucial role in the future of biosynthetic pharmaceutical production.
CitationInternational Journal of Molecular Sciences, volume 22, issue 21, page e11380
DescriptionFrom MDPI via Jisc Publications Router
History: accepted 2021-10-12, pub-electronic 2021-10-21
Publication status: Published
Funder: Biotechnology and Biological Sciences Research Council; Grant(s): BB/M011208/1
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