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dc.contributor.authorWareing, Mark
dc.contributor.authorSmith, Craig P.; email: craig.smith@manchester.ac.uk
dc.date.accessioned2021-10-14T07:30:35Z
dc.date.available2021-10-14T07:30:35Z
dc.date.issued2021-09-30
dc.date.submitted2021-07-13
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/626097/additional-files.zip?sequence=2
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/626097/fphys-12-740716.pdf?sequence=3
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/626097/fphys-12-740716.xml?sequence=4
dc.identifier.citationFrontiers in Physiology, volume 12, page 740716
dc.identifier.urihttp://hdl.handle.net/10034/626097
dc.descriptionFrom Frontiers via Jisc Publications Router
dc.descriptionHistory: collection 2021, received 2021-07-13, accepted 2021-08-30, epub 2021-09-30
dc.descriptionPublication status: Published
dc.description.abstractThe aim of this study was to determine the iron (Fe) concentration profile within the lumen of the S2 renal proximal convoluted tubule (PCT) and to resolve whether this nephron segment transported Fe. To do this, we performed in vivo renal micropuncture on Wistar rats, collected PCT tubular fluid from superficial nephrons, and measured Fe concentration. The Fe concentration profile along the S2 PCT suggested significant Fe reabsorption. Proximal tubules were also microperfused in vivo with physiological solutions containing Fe and Zn, Cu, Mn, or Cd. PCTs perfused with 12μmol.l−1 55FeCl3 reabsorbed 105.2±12.7 fmol.mm−1.min−1 Fe, 435±52pmol.mm-1.min−1 Na, and 2.7±0.2nl.mm−1.min−1 water (mean ± SEM; n=19). Addition of ascorbate (1mmol.l−1) to the perfusate did not significantly alter Fe, Na, or water reabsorption. Supplementing the control perfusate with 60μmol.l−1 FeSO4 significantly decreased 55Fe uptake. Recalculating for the altered molar activity following addition of unlabeled Fe revealed a three-fold increase in Fe flux. Addition to the perfusate 12μmol.l−1 CuSO4, MnSO4, CdSO4, or ZnSO4 did not affect Fe, Na, or water flux. In conclusion, (1) in vivo, S2 PCTs of rat reabsorb Fe and (2) Fe is reabsorbed along the PCT via a pathway that is insensitive to Cu, Mn, Cd, or Zn. Together, these data demonstrate for the first time the hitherto speculated process of renal Fe filtration and subsequent tubular Fe reabsorption in a living mammal.
dc.languageen
dc.publisherFrontiers Media S.A.
dc.rightsLicence for this article: http://creativecommons.org/licenses/by/4.0/
dc.sourceeissn: 1664-042X
dc.subjectPhysiology
dc.subjectkidney
dc.subjectiron
dc.subjectmicropuncture
dc.subjectproximal tubule
dc.subjectglomerular filtration
dc.subjectDMT1
dc.subjectZIP8
dc.subjectZIP14
dc.titleIron Is Filtered by the Kidney and Is Reabsorbed by the Proximal Tubule
dc.typearticle
dc.date.updated2021-10-14T07:30:35Z
dc.date.accepted2021-08-30


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