Show simple item record

dc.contributor.authorBeech, Augusta; orcid: 0000-0002-2690-5364; email: augusta.beech@manchester.ac.uk
dc.contributor.authorLea, Simon; orcid: 0000-0003-3700-1886; email: Simon.lea@manchester.ac.uk
dc.contributor.authorLi, Jian; email: Jian.Li@manchester.ac.uk
dc.contributor.authorJackson, Natalie; email: njackson@meu.org.uk
dc.contributor.authorMulvanny, Alex; email: amulvanny@meu.org.uk
dc.contributor.authorSingh, Dave; email: dsingh@meu.org.uk
dc.date.accessioned2021-09-29T03:36:42Z
dc.date.available2021-09-29T03:36:42Z
dc.date.issued2021-09-27
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625980/biomedicines-09-01337.pdf?sequence=2
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625980/biomedicines-09-01337.xml?sequence=3
dc.identifier.citationBiomedicines, volume 9, issue 10, page e1337
dc.identifier.urihttp://hdl.handle.net/10034/625980
dc.descriptionFrom MDPI via Jisc Publications Router
dc.descriptionHistory: accepted 2021-09-22, pub-electronic 2021-09-27
dc.descriptionPublication status: Published
dc.descriptionFunder: AstraZeneca; Grant(s): ESR-16-11869
dc.description.abstractBackground: Chronic obstructive pulmonary disease (COPD) inflammatory endotypes are associated with different airway microbiomes. We used quantitative polymerase chain reaction (qPCR) analysis of sputum samples to establish the bacterial load upper limit in healthy controls; these values determined the bacterial colonisation prevalence in a longitudinal COPD cohort. Bacteriology combined with sputum inflammatory cells counts were used to investigate COPD endotypes. Methods: Sixty COPD patients and 15 healthy non-smoking controls were recruited. Sputum was analysed by qPCR (for Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and Psuedomonas aeruginosa) and sputum differential cell counts at baseline and 6 months. Results: At baseline and 6 months, 23.1% and 25.6% of COPD patients were colonised with H. influenzae, while colonisation with other bacterial species was less common, e.g., S. pneumoniae—1.9% and 5.1%, respectively. H. influenzae + ve patients had higher neutrophil counts at baseline (90.1% vs. 67.3%, p 0.01), with similar results at 6 months. COPD patients with sputum eosinophil counts ≥3% at ≥1 visit rarely showed bacterial colonisation. Conclusions: The prevalence of H. influenzae colonisation was approximately 25%, with low colonisation for other bacterial species. H. influenzae colonisation was associated with sputum neutrophilia, while eosinophilic inflammation and H. influenzae colonisation rarely coexisted.
dc.languageen
dc.publisherMDPI
dc.rightsLicence for this article: https://creativecommons.org/licenses/by/4.0/
dc.sourceeissn: 2227-9059
dc.subjectchronic obstructive pulmonary disease
dc.subjectinflammatory endotypes
dc.subjectHaemophilus influenzae
dc.subjecteosinophil
dc.subjecteosinophilic inflammation
dc.subjectneutrophil
dc.subjectneutrophilic inflammation
dc.subjectsputum
dc.subjectairway colonisation
dc.titleAirway Bacteria Quantification Using Polymerase Chain Reaction Combined with Neutrophil and Eosinophil Counts Identifies Distinct COPD Endotypes
dc.typearticle
dc.date.updated2021-09-29T03:36:42Z
dc.date.accepted2021-09-22


Files in this item

Thumbnail
Name:
biomedicines-09-01337.pdf
Size:
1.258Mb
Format:
PDF
Thumbnail
Name:
biomedicines-09-01337.xml
Size:
9.936Kb
Format:
XML

This item appears in the following Collection(s)

Show simple item record