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dc.contributor.authorBirmingham, William R; orcid: 0000-0002-8880-5502
dc.contributor.authorToftgaard Pedersen, Asbjørn; orcid: 0000-0002-5283-3210
dc.contributor.authorDias Gomes, Mafalda
dc.contributor.authorBøje Madsen, Mathias
dc.contributor.authorBreuer, Michael
dc.contributor.authorWoodley, John M; orcid: 0000-0002-7976-2483
dc.contributor.authorTurner, Nicholas J; orcid: 0000-0002-8708-0781; email: nicholas.turner@manchester.ac.uk
dc.date.accessioned2021-09-18T00:49:24Z
dc.date.available2021-09-18T00:49:24Z
dc.date.issued2021-08-16
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625881/article.pdf?sequence=2
dc.identifier.citationNature communications, volume 12, issue 1, page 4946
dc.identifier.urihttp://hdl.handle.net/10034/625881
dc.descriptionFrom Europe PMC via Jisc Publications Router
dc.descriptionHistory: ppub 2021-08-01, epub 2021-08-16
dc.descriptionPublication status: Published
dc.description.abstract5-Hydroxymethylfurfural (HMF) has emerged as a crucial bio-based chemical building block in the drive towards developing materials from renewable resources, due to its direct preparation from sugars and its readily diversifiable scaffold. A key obstacle in transitioning to bio-based plastic production lies in meeting the necessary industrial production efficiency, particularly in the cost-effective conversion of HMF to valuable intermediates. Toward addressing the challenge of developing scalable technology for oxidizing crude HMF to more valuable chemicals, here we report coordinated reaction and enzyme engineering to provide a galactose oxidase (GOase) variant with remarkably high activity toward HMF, improved O<sub>2</sub> binding and excellent productivity (>1,000,000 TTN). The biocatalyst and reaction conditions presented here for GOase catalysed selective oxidation of HMF to 2,5-diformylfuran offers a productive blueprint for further development, giving hope for the creation of a biocatalytic route to scalable production of furan-based chemical building blocks from sustainable feedstocks.
dc.languageeng
dc.rightsLicence for this article: cc by
dc.sourceessn: 2041-1723
dc.sourcenlmid: 101528555
dc.sourceissn: 2041-1723
dc.subjectFuraldehyde
dc.subjectFurans
dc.subjectGalactose Oxidase
dc.subjectProtein Engineering
dc.subjectMutagenesis
dc.subjectCatalytic Domain
dc.subjectOxidation-Reduction
dc.subjectCatalysis
dc.subjectBiocatalysis
dc.titleToward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering.
dc.typearticle
dc.date.updated2021-09-18T00:49:24Z


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