Show simple item record

dc.contributor.authorBirmingham, William R.; orcid: 0000-0002-8880-5502
dc.contributor.authorToftgaard Pedersen, Asbjørn; orcid: 0000-0002-5283-3210
dc.contributor.authorDias Gomes, Mafalda
dc.contributor.authorBøje Madsen, Mathias
dc.contributor.authorBreuer, Michael
dc.contributor.authorWoodley, John M.; orcid: 0000-0002-7976-2483
dc.contributor.authorTurner, Nicholas J.; orcid: 0000-0002-8708-0781; email: nicholas.turner@manchester.ac.uk
dc.date.accessioned2021-08-16T15:41:50Z
dc.date.available2021-08-16T15:41:50Z
dc.date.issued2021-08-16
dc.date.submitted2020-12-09
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625605/additional-files.zip?sequence=2
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625605/41467_2021_25034_MOESM3_ESM.pdf?sequence=3
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625605/41467_2021_Article_25034_nlm.xml?sequence=4
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625605/41467_2021_Article_25034.pdf?sequence=5
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625605/41467_2021_25034_MOESM2_ESM.pdf?sequence=6
dc.identifier.citationNature Communications, volume 12, issue 1, page 4946
dc.identifier.urihttp://hdl.handle.net/10034/625605
dc.descriptionFrom Springer Nature via Jisc Publications Router
dc.descriptionHistory: received 2020-12-09, accepted 2021-07-06, registration 2021-07-21, pub-electronic 2021-08-16, online 2021-08-16, collection 2021-12
dc.descriptionPublication status: Published
dc.descriptionFunder: EC | EC Seventh Framework Programm | FP7 Food, Agriculture and Fisheries, Biotechnology (FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Fisheries, Biotechnology); doi: https://doi.org/10.13039/100011262; Grant(s): 613849
dc.description.abstractAbstract: 5-Hydroxymethylfurfural (HMF) has emerged as a crucial bio-based chemical building block in the drive towards developing materials from renewable resources, due to its direct preparation from sugars and its readily diversifiable scaffold. A key obstacle in transitioning to bio-based plastic production lies in meeting the necessary industrial production efficiency, particularly in the cost-effective conversion of HMF to valuable intermediates. Toward addressing the challenge of developing scalable technology for oxidizing crude HMF to more valuable chemicals, here we report coordinated reaction and enzyme engineering to provide a galactose oxidase (GOase) variant with remarkably high activity toward HMF, improved O2 binding and excellent productivity (>1,000,000 TTN). The biocatalyst and reaction conditions presented here for GOase catalysed selective oxidation of HMF to 2,5-diformylfuran offers a productive blueprint for further development, giving hope for the creation of a biocatalytic route to scalable production of furan-based chemical building blocks from sustainable feedstocks.
dc.languageen
dc.publisherNature Publishing Group UK
dc.rightsLicence for this article: http://creativecommons.org/licenses/by/4.0/
dc.sourceeissn: 2041-1723
dc.subjectArticle
dc.subject/639/638/45/607
dc.subject/639/638/92/603
dc.subject/82/16
dc.subject/82/47
dc.subject/82/80
dc.subject/49/22
dc.subjectarticle
dc.titleToward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering
dc.typearticle
dc.date.updated2021-08-16T15:41:50Z
dc.date.accepted2021-07-06


Files in this item

Thumbnail
Name:
additional-files.zip
Size:
17.52Mb
Format:
Unknown
Thumbnail
Name:
41467_2021_25034_MOESM3_ESM.pdf
Size:
69.04Kb
Format:
PDF
Thumbnail
Name:
41467_2021_Article_25034_nlm.xml
Size:
142.3Kb
Format:
XML
Thumbnail
Name:
41467_2021_Article_25034.pdf
Size:
795.9Kb
Format:
PDF
Thumbnail
Name:
41467_2021_25034_MOESM2_ESM.pdf
Size:
195.9Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record