Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering
Authors
Birmingham, William R.; orcid: 0000-0002-8880-5502Toftgaard Pedersen, Asbjørn; orcid: 0000-0002-5283-3210
Dias Gomes, Mafalda
Bøje Madsen, Mathias
Breuer, Michael
Woodley, John M.; orcid: 0000-0002-7976-2483
Turner, Nicholas J.; orcid: 0000-0002-8708-0781; email: nicholas.turner@manchester.ac.uk
Publication Date
2021-08-16Submitted date
2020-12-09
Metadata
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Abstract: 5-Hydroxymethylfurfural (HMF) has emerged as a crucial bio-based chemical building block in the drive towards developing materials from renewable resources, due to its direct preparation from sugars and its readily diversifiable scaffold. A key obstacle in transitioning to bio-based plastic production lies in meeting the necessary industrial production efficiency, particularly in the cost-effective conversion of HMF to valuable intermediates. Toward addressing the challenge of developing scalable technology for oxidizing crude HMF to more valuable chemicals, here we report coordinated reaction and enzyme engineering to provide a galactose oxidase (GOase) variant with remarkably high activity toward HMF, improved O2 binding and excellent productivity (>1,000,000 TTN). The biocatalyst and reaction conditions presented here for GOase catalysed selective oxidation of HMF to 2,5-diformylfuran offers a productive blueprint for further development, giving hope for the creation of a biocatalytic route to scalable production of furan-based chemical building blocks from sustainable feedstocks.Citation
Nature Communications, volume 12, issue 1, page 4946Publisher
Nature Publishing Group UKType
articleDescription
From Springer Nature via Jisc Publications RouterHistory: received 2020-12-09, accepted 2021-07-06, registration 2021-07-21, pub-electronic 2021-08-16, online 2021-08-16, collection 2021-12
Publication status: Published
Funder: EC | EC Seventh Framework Programm | FP7 Food, Agriculture and Fisheries, Biotechnology (FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Fisheries, Biotechnology); doi: https://doi.org/10.13039/100011262; Grant(s): 613849