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dc.contributor.authorClough, Helen C; email: helen.clough@manchester.ac.uk
dc.contributor.authorO'Brien, Marie; email: marie.obrien@manchester.ac.uk
dc.contributor.authorZhu, Xinyi; email: xinyi.zhu@manchester.ac.uk
dc.contributor.authorMiller, Aline F; email: a.miller@manchesterbiogel.com
dc.contributor.authorSaiani, Alberto; email: a.saiani@manchester.ac.uk
dc.contributor.authorTsigkou, Olga; email: olga.tsigkou@manchester.ac.uk
dc.date.accessioned2021-07-20T13:50:04Z
dc.date.available2021-07-20T13:50:04Z
dc.date.issued2021-05-21
dc.date.submitted2021-02-18
dc.identifierpubmed: 34225853
dc.identifierpii: S0928-4931(21)00340-4
dc.identifierdoi: 10.1016/j.msec.2021.112200
dc.identifier.citationMaterials science & engineering. C, Materials for biological applications, volume 127, page 112200
dc.identifier.urihttp://hdl.handle.net/10034/625323
dc.descriptionFrom PubMed via Jisc Publications Router
dc.descriptionHistory: received 2021-02-18, revised 2021-05-05, accepted 2021-05-16
dc.descriptionPublication status: ppublish
dc.description.abstractSelf-assembling peptide hydrogels (SAPH) are a popular biomaterial due to their biocompatibility with a wide range of cell types, synthetic design, structural properties that provide a more accurate 3D microenvironment, and potential for cell- and/or drug-delivery system. Mimicking solid tumors in vitro using hydrogels is one method of testing anti-cancer drug efficacy and observing cancerous cell-ECM interactions within a 3D system. In this study, a SAPH, PeptiGel®Alpha1, was used to model in vitro the 3D breast tumor microenvironment. PeptiGel®Alpha1 is composed of entangled nanofibers with consistent diameter and mechanical properties similar to breast cancer that more accurately mimic the stiffness of breast tumor tissue than Matrigel® or collagen type I. PeptiGel®Alpha1 supported the viability and growth of the breast cancer cell lines MCF-7 and MDA-MB-231 and recapitulated key features of solid tumors such as hypoxia and invasion. MCF-7 cells in the hydrogels formed large spheroids resembling acini, while MDA-MB-231 remained dispersed. When treated with tamoxifen, PeptiGel®Alpha1 acted as a barrier, providing drug penetration geometry similar to that in vivo, providing better prediction of the drug effect. Finally, it was observed that MCF-7 cells engulfed the peptide matrix after 14 days, highlighting a potential use in drug delivery. PeptiGel®Alpha1 is a suitable platform for in vitro modeling of breast cancer. [Abstract copyright: Copyright © 2021. Published by Elsevier B.V.]
dc.languageeng
dc.sourceeissn: 1873-0191
dc.subjectBreast cancer
dc.subjectSelf-assembling peptide hydrogel
dc.subjectTumor microenvironment
dc.subjectin vitro model
dc.titleNeutrally charged self-assembling peptide hydrogel recapitulates in vitro mechanisms of breast cancer progression.
dc.typearticle
dc.date.updated2021-07-20T13:50:04Z
dc.date.accepted2021-05-16


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