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dc.contributor.authorLea, Simon; orcid: 0000-0003-3700-1886; email: Simon.lea@manchester.ac.uk
dc.contributor.authorGaskell, Rosemary
dc.contributor.authorHall, Simon
dc.contributor.authorMaschera, Barbara
dc.contributor.authorHessel, Edith
dc.contributor.authorSingh, Dave
dc.date.accessioned2021-07-12T10:10:51Z
dc.date.available2021-07-12T10:10:51Z
dc.date.issued2021-07-12
dc.date.submitted2020-11-30
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625228/cei.13638.xml?sequence=2
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/625228/cei.13638.pdf?sequence=3
dc.identifier.citationClinical & Experimental Immunology
dc.identifier.urihttp://hdl.handle.net/10034/625228
dc.descriptionFrom Wiley via Jisc Publications Router
dc.descriptionHistory: received 2020-11-30, accepted 2021-06-15, rev-recd 2021-06-15, pub-electronic 2021-07-12
dc.descriptionArticle version: VoR
dc.descriptionPublication status: Published
dc.descriptionFunder: GlaxoSmithKline; Id: http://dx.doi.org/10.13039/100004330
dc.description.abstractAbstract: Defective phagocytosis has been shown in chronic obstructive pulmonary disease (COPD) bronchoalveolar lavage and blood monocyte‐derived macrophages. Phagocytic capabilities of sputum macrophages and neutrophils in COPD are unknown. We investigated phagocytosis in these cells from COPD patients and controls. Phagocytosis of Streptococcus pneumoniae or fluorescently labelled non‐typeable Haemophilus influenzae (NTHi) by sputum macrophages and neutrophils was determined by gentamycin protection assay (COPD; n = 5) or flow cytometry in 14 COPD patients, eight healthy smokers (HS) and nine healthy never‐smokers (HNS). Sputum macrophages and neutrophils were differentiated by adherence for the gentamycin protection assay or receptor expression (CD206 and CD66b, respectively), by flow cytometry. The effects of NTHi on macrophage expression of CD206 and CD14 and neutrophil expression of CD16 were determined by flow cytometry. There was greater uptake of S. pneumoniae [~10‐fold more colony‐forming units (CFU)/ml] by sputum neutrophils compared to macrophages in COPD patients. Flow cytometry showed greater NTHi uptake by neutrophils compared to macrophages in COPD (67 versus 38%, respectively) and HS (61 versus 31%, respectively). NTHi uptake by macrophages was lower in HS (31%, p = 0.019) and COPD patients (38%, p = 0.069) compared to HNS (57%). NTHi uptake by neutrophils was similar between groups. NTHi exposure reduced CD206 and CD14 expression on macrophages and CD16 expression on neutrophils. Sputum neutrophils showed more phagocytic activity than macrophages. There was some evidence that bacterial phagocytosis was impaired in HS sputum macrophages, but no impairment of neutrophils was observed in HS or COPD patients. These results highlight the relative contributions of neutrophils and macrophages to bacterial clearance in COPD.
dc.languageen
dc.rightsLicence for VoR version of this article: http://creativecommons.org/licenses/by-nc/4.0/
dc.sourceissn: 0009-9104
dc.sourceissn: 1365-2249
dc.subjectORIGINAL ARTICLE
dc.subjectORIGINAL ARTICLES
dc.subjectCOPD
dc.subjectmacrophages
dc.subjectneutrophils
dc.subjectphagocytosis
dc.titleAssessment of bacterial exposure on phagocytic capability and surface marker expression of sputum macrophages and neutrophils in COPD patients
dc.typearticle
dc.date.updated2021-07-12T10:10:51Z
dc.date.accepted2021-06-15


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