Show simple item record

dc.contributor.authorMangas-Sanchez, Juan; orcid: 0000-0002-3364-1512
dc.contributor.authorSharma, Mahima; orcid: 0000-0003-3960-2212
dc.contributor.authorCosgrove, Sebastian C; orcid: 0000-0001-9541-7201
dc.contributor.authorRamsden, Jeremy I
dc.contributor.authorMarshall, James R
dc.contributor.authorThorpe, Thomas W
dc.contributor.authorPalmer, Ryan B
dc.contributor.authorGrogan, Gideon
dc.contributor.authorTurner, Nicholas J; orcid: 0000-0002-8708-0781
dc.date.accessioned2021-06-28T00:52:50Z
dc.date.available2021-06-28T00:52:50Z
dc.date.issued2020-05-05
dc.identifierpubmed: 34122962
dc.identifierdoi: 10.1039/d0sc02253e
dc.identifierpii: d0sc02253e
dc.identifierpmc: PMC8159254
dc.identifier.citationChemical science, volume 11, issue 19, page 5052-5057
dc.identifier.urihttp://hdl.handle.net/10034/625064
dc.descriptionFrom PubMed via Jisc Publications Router
dc.descriptionPublication status: epublish
dc.description.abstractChiral primary amines are important intermediates in the synthesis of pharmaceutical compounds. Fungal reductive aminases (RedAms) are NADPH-dependent dehydrogenases that catalyse reductive amination of a range of ketones with short-chain primary amines supplied in an equimolar ratio to give corresponding secondary amines. Herein we describe structural and biochemical characterisation as well as synthetic applications of two RedAms from spp. ( RedAm and RedAm) that display a distinctive activity amongst fungal RedAms, namely a superior ability to use ammonia as the amine partner. Using these enzymes, we demonstrate the synthesis of a broad range of primary amines, with conversions up to >97% and excellent enantiomeric excess. Temperature dependent studies showed that these homologues also possess greater thermal stability compared to other enzymes within this family. Their synthetic applicability is further demonstrated by the production of several primary and secondary amines with turnover numbers (TN) up to 14 000 as well as continous flow reactions, obtaining chiral amines such as ( )-2-aminohexane in space time yields up to 8.1 g L h . The remarkable features of RedAm RedAm highlight their potential for wider synthetic application as well as expanding the biocatalytic toolbox available for chiral amine synthesis. [Abstract copyright: This journal is © The Royal Society of Chemistry.]
dc.languageeng
dc.sourcepissn: 2041-6520
dc.titleAsymmetric synthesis of primary amines catalyzed by thermotolerant fungal reductive aminases.
dc.typearticle
dc.date.updated2021-06-28T00:52:50Z


This item appears in the following Collection(s)

Show simple item record