Show simple item record

dc.contributor.authorMancuso, Elena; email: e.mancuso@ulster.ac.uk
dc.contributor.authorShah, Lekha
dc.contributor.authorJindal, Swati
dc.contributor.authorSerenelli, Cecile
dc.contributor.authorTsikriteas, Zois Michail
dc.contributor.authorKhanbareh, Hamideh
dc.contributor.authorTirella, Annalisa; email: annalisa.tirella@manchester.ac.uk
dc.date.accessioned2021-06-18T00:35:07Z
dc.date.available2021-06-18T00:35:07Z
dc.date.issued2021-05-19
dc.date.submitted2021-04-02
dc.identifierpubmed: 34082989
dc.identifierpii: S0928-4931(21)00331-3
dc.identifierdoi: 10.1016/j.msec.2021.112192
dc.identifier.citationMaterials science & engineering. C, Materials for biological applications, volume 126, page 112192
dc.identifier.urihttp://hdl.handle.net/10034/624977
dc.descriptionFrom PubMed via Jisc Publications Router
dc.descriptionHistory: received 2021-04-02, revised 2021-05-09, accepted 2021-05-14
dc.descriptionPublication status: ppublish
dc.description.abstractPiezoelectric ceramics, such as BaTiO , have gained considerable attention in bone tissue engineering applications thanks to their biocompatibility, ability to sustain a charged surface as well as improve bone cells' adhesion and proliferation. However, the poor processability and brittleness of these materials hinder the fabrication of three-dimensional scaffolds for load bearing tissue engineering applications. For the first time, this study focused on the fabrication and characterisation of BaTiO composite scaffolds by using a multi-material 3D printing technology. Polycaprolactone (PCL) was selected and used as dispersion phase for its low melting point, easy processability and wide adoption in bone tissue engineering. The proposed single-step extrusion-based strategy enabled a faster and solvent-free process, where raw materials in powder forms were mechanically mixed and subsequently fed into the 3D printing system for further processing. PCL, PCL/hydroxyapatite and PCL/BaTiO composite scaffolds were successfully produced with high level of consistency and an inner architecture made of seamlessly integrated layers. The inclusion of BaTiO ceramic particles (10% wt.) significantly improved the mechanical performance of the scaffolds (54 ± 0.5 MPa) compared to PCL/hydroxyapatite scaffolds (40.4 ± 0.1 MPa); moreover, the presence of BaTiO increased the dielectric permittivity over the entire frequency spectrum and tested temperatures. Human osteoblasts Saos-2 were seeded on scaffolds and cellular adhesion, proliferation, differentiation and deposition of bone-like extracellular matrix were evaluated. All tested scaffolds (PCL, PCL/hydroxyapatite and PCL/BaTiO ) supported cell growth and viability, preserving the characteristic cellular osteoblastic phenotype morphology, with PCL/BaTiO composite scaffolds exhibiting higher mineralisation (ALP activity) and deposited bone-like extracellular matrix (osteocalcin and collagen I). The single-step multi-material additive manufacturing technology used for the fabrication of electroactive PCL/BaTiO composite scaffolds holds great promise for sustainability (reduced material waste and manufacturing costs) and it importantly suggests PCL/BaTiO scaffolds as promising candidates for load bearing bone tissue engineering applications to solve unmet clinical needs. [Abstract copyright: Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.]
dc.languageeng
dc.sourceeissn: 1873-0191
dc.subject
dc.subjectAdditive manufacturing
dc.subjectBarium titanate
dc.subjectBone tissue engineering
dc.subjectComposite scaffolds
dc.subjectExtrusion-based technology
dc.subjectPCL
dc.titleAdditively manufactured BaTiO
dc.typearticle
dc.date.updated2021-06-18T00:35:07Z
dc.date.accepted2021-05-14


This item appears in the following Collection(s)

Show simple item record