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dc.contributor.authorSingh, Pawan
dc.contributor.authorHalova, Lenka
dc.contributor.authorHagan, Iain Michael; email: iain.hagan@cruk.manchester.ac.uk
dc.date.accessioned2021-06-18T00:35:06Z
dc.date.available2021-06-18T00:35:06Z
dc.date.issued2021
dc.identifierpubmed: 34085220
dc.identifierdoi: 10.1007/978-1-0716-1538-6_10
dc.identifier.citationMethods in molecular biology (Clifton, N.J.), volume 2329, page 123-142
dc.identifier.urihttp://hdl.handle.net/10034/624976
dc.descriptionFrom PubMed via Jisc Publications Router
dc.descriptionPublication status: ppublish
dc.description.abstractSynchronized progression of a cell population through the cell division cycle supports the biochemical and functional dissection of cell cycle controls and execution. The concerted behaviour of the population reflects the attributes of each cell within that population. The reversible imposition of a block to cell cycle progression at the G2-M boundary through transient inactivation of the Cdk1-Cyclin B activating phosphatase, Cdc25, with the temperature sensitive cdc25-22 mutant, has been widely used to study fission yeast mitosis and DNA replication. However, the biology of the compromised Cdc25-22 phosphatase generates significant division abnormalities upon release from mitotic arrest. We show how reversible inhibition of Cdc2-asM17, with the ATP analog 3-BrB-PP1, generates higher levels of synchrony with timing and morphology much more reminiscent of a normal division. We also describe a version of the H1 kinase assay of Cdk1-Cyclin B activity that is widely used to monitor mitotic progression which does not require radiolabeled ATP.
dc.languageeng
dc.sourceeissn: 1940-6029
dc.subjectCdc2
dc.subjectCdc25
dc.subjectCell cycle
dc.subjectFission yeast
dc.subjectG2–M
dc.subjectHistone H1 kinase assay
dc.subjectMitosis
dc.subjectP-Thr-Pro-101
dc.subjectS. pombe
dc.subjectSynchronization
dc.titleHighly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition.
dc.typearticle
dc.date.updated2021-06-18T00:35:06Z


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