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dc.contributor.authorKanakis, Ioannis; orcid: 0000-0001-6410-1482
dc.contributor.authorAlameddine, Moussira
dc.contributor.authorFolkes, Leighton; orcid: 0000-0002-4009-6540
dc.contributor.authorMoxon, Simon; orcid: 0000-0003-4644-1816
dc.contributor.authorMyrtziou, Ioanna
dc.contributor.authorOzanne, Susan E
dc.contributor.authorPeffers, Mandy J; orcid: 0000-0001-6979-0440
dc.contributor.authorGoljanek-Whysall, Katarzyna; orcid: 0000-0001-8166-8800
dc.contributor.authorVasilaki, Aphrodite
dc.date.accessioned2021-06-17T09:59:05Z
dc.date.available2021-06-17T09:59:05Z
dc.date.issued2021-05-11
dc.date.submitted2021-04-13
dc.identifierpubmed: 34064819
dc.identifierpii: cells10051166
dc.identifierdoi: 10.3390/cells10051166
dc.identifierpmc: PMC8150574
dc.identifier.citationCells, volume 10, issue 5
dc.identifier.urihttp://hdl.handle.net/10034/624959
dc.descriptionFrom PubMed via Jisc Publications Router
dc.descriptionHistory: received 2021-04-13, revised 2021-05-07, accepted 2021-05-09
dc.descriptionPublication status: epublish
dc.descriptionFunder: Biotechnology and Biological Sciences Research Council; Grant(s): BB/P008429/1
dc.description.abstractMaternal diet during gestation and lactation affects the development of skeletal muscles in offspring and determines muscle health in later life. In this paper, we describe the association between maternal low protein diet-induced changes in offspring skeletal muscle and the differential expression (DE) of small non-coding RNAs (sncRNAs). We used a mouse model of maternal protein restriction, where dams were fed either a normal (N, 20%) or a low protein (L, 8%) diet during gestation and newborns were cross-fostered to N or L lactating dams, resulting in the generation of NN, NL and LN offspring groups. Total body and tibialis anterior (TA) weights were decreased in weanling NL male offspring but were not different in the LN group, as compared to NN. However, histological evaluation of TA muscle revealed reduced muscle fibre size in both groups at weaning. Small RNA-sequencing demonstrated DE of multiple miRs, snoRNAs and snRNAs. Bioinformatic analyses of miRs-15a, -34a, -122 and -199a, in combination with known myomiRs, confirmed their implication in key muscle-specific biological processes. This is the first comprehensive report for the DE of sncRNAs in nutrition-associated programming of skeletal muscle development, highlighting the need for further research to unravel the detailed molecular mechanisms.
dc.languageeng
dc.sourceeissn: 2073-4409
dc.subjectmaternal protein restriction
dc.subjectmicroRNAs
dc.subjectmuscle development
dc.subjectoffspring
dc.subjectsncRNAs
dc.subjectsnoRNAs
dc.titleSmall-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation.
dc.typearticle
dc.date.updated2021-06-17T09:59:05Z
dc.date.accepted2021-05-09


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