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dc.contributor.authorHall, C; orcid: 0000-0002-0491-4438
dc.contributor.authorvon Grabowiecki, Y; orcid: 0000-0003-2189-6953
dc.contributor.authorPearce, S P; orcid: 0000-0002-1680-5538
dc.contributor.authorDive, C
dc.contributor.authorBagley, S; orcid: 0000-0002-9007-7292
dc.contributor.authorMuller, P A J; orcid: 0000-0002-0926-1499; email: patricia.muller@cruk.manchester.ac.uk
dc.date.accessioned2021-06-05T00:57:54Z
dc.date.available2021-06-05T00:57:54Z
dc.date.issued2021-05-03
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/624849/article.pdf?sequence=2
dc.identifier.citationCancer cell international, volume 21, issue 1, page 247
dc.identifier.urihttp://hdl.handle.net/10034/624849
dc.descriptionFrom Europe PMC via Jisc Publications Router
dc.descriptionHistory: ppub 2021-05-01, epub 2021-05-03
dc.descriptionPublication status: Published
dc.descriptionFunder: Wellcome Trust; Grant(s): 101242/Z/13/Z
dc.description.abstract<h4>Background</h4>In vivo imaging using fluorescence is used in cancer biology for the detection, measurement and monitoring of tumours. This can be achieved with the expression of fluorescent proteins such as iRFP, which emits light at a wavelength less attenuated in biological tissues compared to light emitted by other fluorescent proteins such as GFP or RFP. Imaging platforms capable of detecting fluorescent tumours in small animals have been developed but studies comparing the performance of these platforms are scarce.<h4>Results</h4>Through access to three platforms from Xenogen, Bruker and Li-Cor, we compared their ability to detect iRFP-expressing subcutaneous tumours as well as tumours localised deeper within the body of female NSG mice. Each platform was paired with proprietary software for image analyse, but the output depends on subjective decisions from the user. To more objectively compare platforms, we developed an 'in house' software-based approach which results in lower measured variability between mice.<h4>Conclusions</h4>Our comparisons showed that all three platforms allowed for reliable detection and monitoring of subcutaneous iRFP tumour growth. The biggest differences between platforms became apparent when imaging deeper tumours with the Li-Cor platform detecting most tumours and showing the highest dynamic range.
dc.languageeng
dc.rightsLicence for this article: cc by
dc.sourceessn: 1475-2867
dc.sourcenlmid: 101139795
dc.sourceissn: 1475-2867
dc.subjectMice
dc.subjectTumours
dc.subjectImaging
dc.subjectIrfp
dc.titleiRFP (near-infrared fluorescent protein) imaging of subcutaneous and deep tissue tumours in mice highlights differences between imaging platforms.
dc.typearticle
dc.date.updated2021-06-05T00:57:54Z


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