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dc.contributor.authorWood, Chelsea R
dc.contributor.authorJuárez, Esri H
dc.contributor.authorFerrini, Francesco
dc.contributor.authorMyint, Peter
dc.contributor.authorInnes, John
dc.contributor.authorLossi, Laura
dc.contributor.authorMerighi, Adalberto
dc.contributor.authorJohnson, William E B
dc.date.accessioned2021-03-29T00:41:29Z
dc.date.available2021-03-29T00:41:29Z
dc.date.issued2021-03-03
dc.date.submitted2020-12-07
dc.identifierpubmed: 33718633
dc.identifierdoi: 10.1016/j.bbrep.2021.100976
dc.identifierpii: S2405-5808(21)00070-4
dc.identifierpmc: PMC7933697
dc.identifier.citationBiochemistry and biophysics reports, volume 26, page 100976
dc.identifier.urihttp://hdl.handle.net/10034/624407
dc.descriptionFrom PubMed via Jisc Publications Router
dc.descriptionHistory: received 2020-12-07, revised 2021-02-22, accepted 2021-02-22
dc.descriptionPublication status: epublish
dc.description.abstractspinal cord slice cultures (SCSC) allow study of spinal cord circuitry, maintaining stimuli responses comparable to live animals. Previously, we have shown that mesenchymal stem/stromal cell (MSC) transplantation reduced inflammation and increased nerve regeneration but MSC survival was short-lived, highlighting that beneficial action may derive from the secretome. Previous studies of MSC conditioned medium (CM) have also shown increased neuronal growth. In this study, murine SCSC were cultured in canine MSC CM (harvested from the adipose tissue of excised inguinal fat) and cell phenotypes analysed via immunohistochemistry and confocal microscopy. SCSC in MSC CM displayed enhanced viability after propidium iodide staining. GFAP immunoreactivity was significantly increased in SCSC in MSC CM compared to controls, but with no change in proteoglycan (NG2) immunoreactivity. In contrast, culture in MSC CM significantly decreased the prevalence of βIII-tubulin immunoreactive neurites, whilst Ca transients per cell were significantly increased. These results contradict previous and reports of how MSC and their secretome may affect the microenvironment of the spinal cord after injury and highlight the importance of a careful comparison of the different experimental conditions used to assess the potential of cell therapies for the treatment of spinal cord injury. [Abstract copyright: © 2021 Published by Elsevier B.V.]
dc.languageeng
dc.sourceeissn: 2405-5808
dc.subjectAstrocyte hypertrophy
dc.subjectCalcium imaging
dc.subjectMesenchymal stem/stromal cell
dc.subjectNeuronal survival
dc.subjectSpinal cord slice cultures
dc.titleMesenchymal stem cell conditioned medium increases glial reactivity and decreases neuronal survival in spinal cord slice cultures.
dc.typearticle
dc.date.updated2021-03-29T00:41:29Z
dc.date.accepted2021-02-22


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