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    Mesenchymal stem cell conditioned medium increases glial reactivity and decreases neuronal survival in spinal cord slice cultures.

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    Authors
    Wood, Chelsea R
    Juárez, Esri H
    Ferrini, Francesco
    Myint, Peter
    Innes, John
    Lossi, Laura
    Merighi, Adalberto
    Johnson, William E B
    Publication Date
    2021-03-03
    Submitted date
    2020-12-07
    
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    Abstract
    spinal cord slice cultures (SCSC) allow study of spinal cord circuitry, maintaining stimuli responses comparable to live animals. Previously, we have shown that mesenchymal stem/stromal cell (MSC) transplantation reduced inflammation and increased nerve regeneration but MSC survival was short-lived, highlighting that beneficial action may derive from the secretome. Previous studies of MSC conditioned medium (CM) have also shown increased neuronal growth. In this study, murine SCSC were cultured in canine MSC CM (harvested from the adipose tissue of excised inguinal fat) and cell phenotypes analysed via immunohistochemistry and confocal microscopy. SCSC in MSC CM displayed enhanced viability after propidium iodide staining. GFAP immunoreactivity was significantly increased in SCSC in MSC CM compared to controls, but with no change in proteoglycan (NG2) immunoreactivity. In contrast, culture in MSC CM significantly decreased the prevalence of βIII-tubulin immunoreactive neurites, whilst Ca transients per cell were significantly increased. These results contradict previous and reports of how MSC and their secretome may affect the microenvironment of the spinal cord after injury and highlight the importance of a careful comparison of the different experimental conditions used to assess the potential of cell therapies for the treatment of spinal cord injury. [Abstract copyright: © 2021 Published by Elsevier B.V.]
    Citation
    Biochemistry and biophysics reports, volume 26, page 100976
    URI
    http://hdl.handle.net/10034/624407
    Type
    article
    Description
    From PubMed via Jisc Publications Router
    History: received 2020-12-07, revised 2021-02-22, accepted 2021-02-22
    Publication status: epublish
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