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dc.contributor.authorMorgan, Amy
dc.contributor.authorAcutt, Katie
dc.contributor.authorMc Auley, Mark
dc.date.accessioned2020-11-18T11:35:31Z
dc.date.available2020-11-18T11:35:31Z
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/623985/Morgan%20et%20al%20For%20Chester%20Rep.pdf?sequence=4
dc.identifier.citationMorgan, A.E., Acutt, K.D., McAuley, M.T. (2020) Electrochemically detecting DNA methylation in the EN1 gene promoter: implications for understanding ageing and disease. Biosci Rep, 40 (11). doi: https://doi.org/10.1042/BSR20202571en_US
dc.identifier.urihttp://hdl.handle.net/10034/623985
dc.descriptionThis is the accepted version of the manuscript and not the final peer-reviewed version.en_US
dc.description.abstractThere is a growing need for biomarkers which predict age-onset pathology. Although this is challenging, the methylome offers significant potential. Cancer is associated with the hypermethylation of many gene promoters, among which are developmental genes. Evolutionary theory suggests developmental genes arbitrate early-late life trade-offs, causing epimutations that increase disease vulnerability. Such genes could predict age related disease. The aim of this work was to optimise an electrochemical procedure for the future investigation of a broad range of ageing related pathologies. An electrochemical approach, which adopted three analytical techniques, was used to investigate DNA methylation in the EN1 gene promoter. Using synthetic single stranded DNA, one technique was able to detect DNA at concentrations as low as 10nM, with methylation status distinguishable at concentrations >25nM. A negative correlation could be observed between % methylation of heterogeneous solution and the key electrochemical parameter, Rct (r = -0.982, p < 0.01). The technique was applied to the breast cancer cell line MCF-7, where a similar correlation was observed (r = -0.965, p < 0.01). These results suggest electrochemistry can effectively measure DNA methylation at low concentrations of DNA. This has implications for the future detection of age-related disease.en_US
dc.publisherPortland Pressen_US
dc.relation.urlhttps://portlandpress.com/bioscirep/article/40/11/BSR20202571/226876/Electrochemically-detecting-DNA-methylation-in-theen_US
dc.rightsCC0 1.0 Universal*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectageingen_US
dc.subjectDNA methylationen_US
dc.subjectcanceren_US
dc.titleElectrochemically Detecting DNA Methylation in the EN1 gene Promoter: Implications for understanding Ageing and Diseaseen_US
dc.typeArticleen_US
dc.identifier.eissn1573-4935en_US
dc.contributor.departmentUniversity of Chesteren_US
dc.identifier.journalBioscience Reportsen_US
or.grant.openaccessYesen_US
rioxxterms.funderunfundeden_US
rioxxterms.identifier.projectunfundeden_US
rioxxterms.versionAMen_US
rioxxterms.versionofrecordhttps://doi.org/10.1042/BSR20202571en_US
rioxxterms.licenseref.startdate2020-11-02
dcterms.dateAccepted2020-10-28
rioxxterms.publicationdate2020-11-02
dc.date.deposited18-11-2020en_US
dc.indentifier.issn0144-8463en_US


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