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dc.contributor.advisorRoberts, Jason
dc.contributor.authorZagkos, Loukas
dc.date.accessioned2020-07-16T14:05:14Z
dc.date.available2020-07-16T14:05:14Z
dc.date.issued2020-03-09
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/623553/FINAL%20phd%20thesis.pdf?sequence=1
dc.identifier.citationZagkos, L. (2020). Mathematical Modelling of DNA Methylation. (Doctoral dissertation). University of Chester, United Kingdom.en_US
dc.identifier.urihttp://hdl.handle.net/10034/623553
dc.description.abstractDNA methylation is a key epigenetic process which has been intimately associated with gene regulation. In recent years growing evidence has associated DNA methylation status with a variety of diseases including cancer, Alzheimer’s disease and cardiovascular disease. Moreover, changes to DNA methylation have also recently been implicated in the ageing process. The factors which underpin DNA methylation are complex, and remain to be fully elucidated. Over the years mathematical modelling has helped to shed light on the dynamics of this important molecular system. Although the existing models have contributed significantly to our overall understanding of DNA methylation, they fall short of fully capturing the dynamics of this process. In this work DNA methylation models are developed and improved and their suitability is demonstrated through mathematical analysis and computational simulation. In particular, a linear and nonlinear deterministic model are developed which capture more fully the dynamics of the key intracellular events which characterise DNA methylation. Furthermore, uncertainty is introduced into the model to describe the presence of intrinsic and extrinsic cell noise. This way a stochastic model is constructed and presented which accounts for the stochastic nature in cell dynamics. One of the key predictions of the model is that DNA methylation dynamics do not alter when the quantity of DNA methylation enzymes change. In addition, the nonlinear model predicts DNA methylation promoter bistability, which is commonly observed experimentally. Moreover, a new way of modelling DNA methylation uncertainty is introduced.en_US
dc.language.isoenen_US
dc.publisherUniversity of Chesteren_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectDNA methylationen_US
dc.subjectmathematical modellingen_US
dc.titleMathematical Modelling of DNA Methylationen_US
dc.typeThesis or dissertationen_US
dc.rights.embargodate2021-01-16
dc.type.qualificationnamePhDen_US
dc.rights.embargoreasonRecommend 6 month embargoen_US
dc.type.qualificationlevelDoctoralen_US


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