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dc.contributor.authorBara, Jennifer
dc.contributor.authorJohnson, William Eustace Basil
dc.contributor.authorRoberts, Sally
dc.contributor.authorMcCarthy, Helen E.
dc.contributor.authorHumphrey, Emma
dc.date.accessioned2020-06-26T09:33:45Z
dc.date.available2020-06-26T09:33:45Z
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/623520/Bara%202014.pdf?sequence=1
dc.identifier.citationBara, J. J., McCarthy, H. E., Humphrey, E., Johnson, W. E. B., & Roberts, S. (2014). Bone marrow-derived mesenchymal stem cells become antiangiogenic when chondrogenically or osteogenically differentiated: Implications for bone and cartilage tissue engineering. Tissue Engineering. Part A, 20(1-2), 147.en_US
dc.identifier.urihttp://hdl.handle.net/10034/623520
dc.description.abstractOsteochondral tissue repair requires formation of vascularized bone and avascular cartilage. Mesenchymal stem cells stimulate angiogenesis both in vitro and in vivo but it is not known if these proangiogenic properties change as a result of chondrogenic or osteogenic differentiation. We investigated the angiogenic/antiangiogenic properties of equine bone marrow-derived mesenchymal stem cells (eBMSCs) before and after differentiation in vitro. Conditioned media from chondrogenic and osteogenic cell pellets and undifferentiated cells was applied to endothelial tube formation assays using Matrigel. Additionally, the cell secretome was analysed using LC-MS/MS mass spectrometry and screened for angiogenesis and neurogenesis-related factors using protein arrays. Endothelial tube-like formation was supported by conditioned media from undifferentiated eBMSCs. Conversely, chondrogenic and osteogenic conditioned media was antiangiogenic as shown by significantly decreased length of endothelial tube-like structures and degree of branching compared to controls. Undifferentiated cells produced higher levels of angiogenesis-related proteins compared to chondrogenic and osteogenic pellets. In summary, eBMSCs produce an array of angiogenesis-related proteins and support angiogenesis in vitro via a paracrine mechanism. However, when these cells are differentiated chondrogenically or osteogenically, they produce a soluble factor(s) that inhibits angiogenesis. With respect to osteochondral tissue engineering, this may be beneficial for avascular articular cartilage formation but unfavourable for bone formation where a vascularized tissue is desired.en_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.urlhttps://www.liebertpub.com/doi/pdf/10.1089/ten.tea.2013.0196en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectStem cellsen_US
dc.subjectcartilageen_US
dc.subjectregenerative medicineen_US
dc.titleBone Marrow-Derived Mesenchymal Stem Cells Become Antiangiogenic When Chondrogenically or Osteogenically Differentiated: Implications for Bone and Cartilage Tissue Engineeringen_US
dc.typeArticleen_US
dc.identifier.eissn1937-335Xen_US
dc.contributor.departmentAO Institute, Davos, Switzerland; Aston University; Keele Universityen_US
dc.identifier.journalTissue Engineering Part Aen_US
or.grant.openaccessYesen_US
rioxxterms.funderEuropean Union (MyJoint Project FP-6 NEST 028861), Keele University and the Institute of Orthopaedics, RJAH Orthopaedic Hospital for funding.en_US
rioxxterms.identifier.projectUnfundeden_US
rioxxterms.versionVoRen_US
rioxxterms.versionofrecord10.1089/ten.tea.2013.0196en_US
rioxxterms.licenseref.startdate2213-09-10
refterms.dateFCD2020-06-25T09:59:55Z
refterms.versionFCDAM
rioxxterms.publicationdate2013-09-10
dc.dateAccepted2013-07-17
dc.date.deposited2020-06-26en_US
dc.indentifier.issn1937-3368en_US


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