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dc.contributor.authorKohli, Nupur
dc.contributor.authorJohnson, William Eustace Basil
dc.contributor.authorUchida, Kenzo
dc.date.accessioned2020-06-26T09:24:15Z
dc.date.available2020-06-26T09:24:15Z
dc.date.issued2019-02-28
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/623519/Kohli%20SCI%20REPORTS%202019.pdf?sequence=1
dc.identifier.citationKohli, N., Al-Delfi, I. R., Snow, M., Sakamoto, T., Miyazaki, T., Nakajima, H., ... & Johnson, W. E. (2019). CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells. Scientific Reports, 9(1), 1-12.en_US
dc.identifier.urihttp://hdl.handle.net/10034/623519
dc.descriptionImportant in vivo study demonstrating the wound healing capacity of prospectively isolated adult stem cells in damaged osteochondral tissues.en_US
dc.description.abstractCD271 is a marker of bone marrow MSCs with enhanced differentiation capacity for bone or cartilage repair. However, the nature of CD271+ MSCs from adipose tissue (AT) is less well understood. Here, we investigated the differentiation, wound healing and angiogenic capacity of plastic adherent MSCs (PA MSCs) versus CD271+ MSCs from AT. There was no difference in the extent to which PA MSCs and CD271+ MSCs formed osteoblasts, adipocytes or chondrocytes in vitro. In contrast, CD271+ MSCs transplanted into athymic rats significantly enhanced osteochondral wound healing with reduced vascularisation in the repair tissue compared to PA MSCs and control animals; there was little histological evidence of mature articular cartilage formation in all animals. Conditioned medium from CD271+ MSC cultures was less angiogenic than PA MSC conditioned medium, and had little effect on endothelial cell migration or endothelial tubule formation in vitro. The low angiogenic activity of CD271+ MSCs and improved early stage tissue repair of osteochondral lesions when transplanted, along with a comparable differentiation capacity along mesenchymal lineages when induced, suggests that these selected cells are a better candidate than PA MSCs for the repair of cartilaginous tissue.en_US
dc.publisherNatureen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectStem cellsen_US
dc.subjectCartilageen_US
dc.subjectRegenerative medicineen_US
dc.titleCD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cellsen_US
dc.typeArticleen_US
dc.identifier.eissn2045-2322en_US
dc.contributor.departmentAston University, University of Chester, University of Fukuien_US
dc.identifier.journalScientific Reportsen_US
or.grant.openaccessYesen_US
rioxxterms.funderFunding was provided by the BBSRC (BB/H016570/1) and the Iraqi Ministry of Higher Education and Research of the Iraq Government.en_US
rioxxterms.identifier.projectBB/H016570/1en_US
rioxxterms.versionAMen_US
rioxxterms.versionofrecordhttps://doi.org/10.1038/s41598-019-39715-z.en_US
rioxxterms.licenseref.startdate2019-02-28
refterms.dateFCD2020-06-25T09:35:59Z
refterms.versionFCDAM
refterms.dateFOA2020-06-26T09:24:16Z
rioxxterms.publicationdate2019-02-28
dc.dateAccepted2019-01-25
dc.date.deposited2020-06-26en_US


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