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dc.contributor.authorJohnson, William Eustace Basil
dc.contributor.authorAl Delfi, Ibtesam
dc.date.accessioned2020-06-26T09:10:50Z
dc.date.available2020-06-26T09:10:50Z
dc.date.issued2016-09-19
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/623516/YTVJL-D-16-00402R4-2%2010%20Sept%202016.pdf?sequence=6
dc.identifier.citationAl Delfi, I. R., Sheard, J. J., Wood, C. R., Vernallis, A., Innes, J. F., Myint, P., & Johnson, W. E. B. (2016). Canine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity. The Veterinary Journal, 217, 10-17.en_US
dc.identifier.doi10.1016/j.tvjl.2016.09.003
dc.identifier.urihttp://hdl.handle.net/10034/623516
dc.descriptionThe lab-based study demonstrates that canine adipose-derived cells secrete factors that are angiogenic and neurogenic using cell assays. This activity has application in neural tissue repair.en_US
dc.description.abstractMesenchymal stem cells (MSCs) have been used in cell replacement therapies for connective tissue damage, but also can stimulate wound healing through paracrine activity. In order to further understand the potential use of MSCs to treat dogs with neurological disorders, this study examined the paracrine action of adipose-derived canine MSCs on neuronal and endothelial cell models. The culture-expanded MSCs exhibited a MSC phenotype according to plastic adherence, cell morphology, CD profiling and differentiation potential along mesenchymal lineages. Treating the SH-SY5Y neuronal cell line with serum-free MSC culture-conditioned medium (MSC CM) significantly increased SH-SY5Y cell proliferation (P <0.01), neurite outgrowth (P = 0.0055) and immunopositivity for the neuronal marker βIII-tubulin (P = 0.0002). Treatment of the EA.hy926 endothelial cell line with MSC CM significantly increased the rate of wound closure in endothelial cell scratch wound assays (P = 0.0409), which was associated with significantly increased endothelial cell proliferation (P <0.05) and migration (P = 0.0001). Furthermore, canine MSC CM induced endothelial tubule formation in EA.hy926 cells in a soluble basement membrane matrix. Hence, this study has demonstrated that adipose-derived canine MSC CM stimulated neuronal and endothelial cells probably through the paracrine activity of MSC-secreted factors. This supports the use of canine MSC transplants or their secreted products in the clinical treatment of dogs with neurological disorders and provides some insight into possible mechanisms of action.en_US
dc.language.isoenen
dc.publisherElsevieren_US
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S1090023316301447?via%3Dihuben_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectStem cellsen_US
dc.subjectSpinal cord injuryen_US
dc.subjectRegenerative medicineen_US
dc.titleCanine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity.en_US
dc.typeArticleen_US
dc.contributor.departmentAston University, University of Chester, Veterinary Tissue Bank Ltden_US
dc.identifier.journalThe Veterinary Journalen_US
dc.date.accepted2016-09-16
or.grant.openaccessYesen_US
rioxxterms.funderBBSRCen_US
rioxxterms.identifier.projectBB/M017311/1en_US
rioxxterms.versionAMen_US
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.tvjl.2016.09.003en_US
rioxxterms.licenseref.startdate2017-09-19
refterms.dateFCD2020-06-23T08:59:47Z
refterms.versionFCDAM
rioxxterms.publicationdate2016-09-19
dc.dateAccepted2016-09-13
dc.date.deposited2020-06-26en_US
dc.indentifier.issn1090-0233en_US


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