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dc.contributor.authorMuir, Anna P.
dc.contributor.authorPhillips, Susanna
dc.contributor.authorGeary, Matt
dc.contributor.authorAllmark, Matthew
dc.contributor.authorBennett, Sarah
dc.contributor.authorNorman, Kim
dc.contributor.authorBall, Rachel J
dc.contributor.authorPeters, Catherine
dc.date.accessioned2020-06-17T08:48:33Z
dc.date.available2020-06-17T08:48:33Z
dc.date.issued2020-07-31
dc.identifierhttps://chesterrep.openrepository.com/bitstream/handle/10034/623500/Natterjack_reintroduction_genetics_RevisionMay2020_clean_refupdate.pdf?sequence=4
dc.identifier.citationPhillips, S., Geary, M., Allmark, M., Bennett, S., Norman, K., Ball, R., Peters, C., Muir, A.P. (2020). The importance of long-term genetic monitoring of reintroduced populations: inbreeding in the natterjack toad (Epidalea calamita). The Herpetological Journal, 30(3), 159-167.en_US
dc.identifier.issn0268-0130
dc.identifier.doi10.33256/hj30.3.159167
dc.identifier.urihttp://hdl.handle.net/10034/623500
dc.description.abstractGenetic monitoring is an important, but frequently lacking, component of management actions to support long-term persistence in reintroduced populations. Populations that remain small, due to demographic processes and genetic diversity, are more likely to experience a second extinction event. The natterjack toad (Epidelea calamita) is legally protected in Britain and was the subject of a reintroduction programme in the 1990s. However, subsequent genetic assessment has been mostly lacking. The aim of this study was to assess the genetic diversity of two reintroduced populations of natterjack toads in order to inform conservation management. Adults were sampled and nine microsatellites amplified to assess neutral genetic variation within each site and for comparison with the source population. Inbreeding was observed at the reintroduction sites, as evidenced by high FIS values (0.43 and 0.72), low observed compared to expected heterozygosities, and significant deviation from Hardy-Weinberg equilibrium. Observed heterozygosity is currently lower in the reintroduction sites than it was in the source population at the time of the reintroductions (Red Rocks: 0.15±0.20; Talacre: 0.12±0.20; Ainsdale (source): 0.29). Evidence for a bottleneck was not found, although this is likely a result of sampling overlapping generations. No within-site population structuring was observed. Such low genetic diversity has not previously been recorded in any natterjack population. Genetic rescue, combined with pool creation, is the most viable option for safeguarding the species at these sites into the future. Our work highlights the importance of ongoing genetic monitoring, in collaboration with conservation organisations, to support conservation management.en_US
dc.publisherBritish Herpetological Societyen_US
dc.relation.urlhttps://www.thebhs.org/publications/the-herpetological-journal/volume-30-number-3-july-2020/3118-05-the-importance-of-long-term-genetic-monitoring-of-reintroduced-populations-inbreeding-in-the-natterjack-toad-i-epidalea-calamita-ien_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectReintroductionen_US
dc.subjectamphibianen_US
dc.subjectinbreedingen_US
dc.subjectconservationen_US
dc.subjectnatterjacken_US
dc.subjectgeneticsen_US
dc.titleThe importance of long-term genetic monitoring of reintroduced populations: inbreeding in the natterjack toad (Epidalea calamita)en_US
dc.typeArticleen_US
dc.contributor.departmentUniversity of Chester; Cheshire Wildlife Trust; Eni UK Ltden_US
dc.identifier.journalThe Herpetological Journalen_US
or.grant.openaccessYesen_US
rioxxterms.funderunfundeden_US
rioxxterms.identifier.projectunfundeden_US
rioxxterms.versionAMen_US
rioxxterms.versionofrecordhttps://doi.org/10.33256/hj30.3.159167
rioxxterms.licenseref.startdate2020-07-31
refterms.dateFCD2020-06-17T08:10:52Z
refterms.versionFCDAM
refterms.dateFOA2020-07-31T00:00:00Z
rioxxterms.publicationdate2020-07-31
dc.dateAccepted2020-05-30
dc.date.deposited2020-06-17en_US


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