Show simple item record

dc.contributor.authorHyland, Mairead
dc.contributor.authorMennan, Claire
dc.contributor.authorWilson, Emma
dc.contributor.authorClayton, Aled
dc.contributor.authorKehoe, Oksana
dc.date.accessioned2020-04-30T10:11:55Z
dc.date.available2020-04-30T10:11:55Z
dc.identifier.citationHyland, M., Mennan, C., Wilson, E., Clayton, A., & Kehoe, O. (2020). Pro-Inflammatory Priming of Umbilical Cord Mesenchymal Stromal Cells Alters the Protein Cargo of Their Extracellular Vesicles. Cells, 9(3), 726. https://doi.org/10.3390/cells9030726en_US
dc.identifier.urihttp://hdl.handle.net/10034/623383
dc.description.abstractUmbilical cord mesenchymal stromal cells (UCMSCs) have shown an ability to modulate the immune system through the secretion of paracrine mediators, such as extracellular vesicles (EVs). However, the culture conditions that UCMSCs are grown in can alter their secretome and thereby affect their immunomodulatory potential. UCMSCs are commonly cultured at 21% O2 in vitro, but recent research is exploring their growth at lower oxygen conditions to emulate circulating oxygen levels in vivo. Additionally, a pro-inflammatory culture environment is known to enhance UCMSC anti-inflammatory potential. Therefore, this paper examined EVs from UCMSCs grown in normal oxygen (21% O2), low oxygen (5% O2) and pro-inflammatory conditions to see the impact of culture conditions on the EV profile. EVs were isolated from UCMSC conditioned media and characterised based on size, morphology and surface marker expression. EV protein cargo was analysed using a proximity-based extension assay. Results showed that EVs had a similar size and morphology. Differences were found in EV protein cargo, with pro-inflammatory primed EVs showing an increase in proteins associated with chemotaxis and angiogenesis. This showed that the UCMSC culture environment could alter the EV protein profile and might have downstream implications for their functions in immunomodulation.en_US
dc.publisherMDPIen_US
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140705/pdf/cells-09-00726.pdfen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectUmbilical cord mesenchymal stromal cellen_US
dc.subjectExtracellular vesiclesen_US
dc.subjectProtein expressionen_US
dc.subjectPro-inflammatory primingen_US
dc.subjectHypoxiaen_US
dc.subjectCulture conditionsen_US
dc.subjectImmunomodulationen_US
dc.titlePro-Inflammatory Priming of Umbilical Cord Mesenchymal Stromal Cells Alters the Protein Cargo of Their Extracellular Vesiclesen_US
dc.typeArticleen_US
dc.identifier.eissn2073-4409en_US
dc.contributor.departmentSchool of Medicine, Keele University, School of Pharmacy and Bioengineering at the RJAH Orthopaedic Hospital, Chester Medical School, School of Medicine, Cardiffen_US
dc.identifier.journalCellsen_US
or.grant.openaccessYesen_US
rioxxterms.funderThis work is supported by the Orthopaedic Institute Ltd., ACORN fund at Keele University and the RJAH Orthopaedic Hospital Charityen_US
rioxxterms.identifier.projectThis work is supported by the Orthopaedic Institute Ltd., ACORN fund at Keele University and the RJAH Orthopaedic Hospital Charityen_US
rioxxterms.versionAMen_US
rioxxterms.versionofrecord10.3390/cells9030726en_US
rioxxterms.licenseref.startdate2020-03-16
rioxxterms.publicationdate2020-03-16
dc.dateAccepted2020-03-12
dc.date.deposited2020-04-30en_US
dc.indentifier.issn2073-4409en_US


Files in this item

Thumbnail
Name:
cells-09-00726.pdf
Size:
1.624Mb
Format:
PDF
Request:
main article
Thumbnail
Name:
Cells Paper_final 13.02.20.pdf
Size:
684.8Kb
Format:
PDF
Request:
Main article

This item appears in the following Collection(s)

Show simple item record

https://creativecommons.org/licenses/by-nc-nd/4.0/
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/