Show simple item record

dc.contributor.authorSebaiy, Mahmoud M
dc.contributor.authorZiedan, Noha I
dc.date.accessioned2019-12-11T01:56:37Z
dc.date.available2019-12-11T01:56:37Z
dc.date.issued2019-11-24
dc.date.submitted2019-08-07
dc.identifierpubmed: 31774044
dc.identifierpii: CDM-EPUB-102564
dc.identifierdoi: 10.2174/1389200220666191125095648
dc.identifier.citationCurrent drug metabolism
dc.identifier.urihttp://hdl.handle.net/10034/622886
dc.descriptionFrom PubMed via Jisc Publications Router
dc.descriptionHistory: received 2019-08-07, revised 2019-09-01, accepted 2019-09-05
dc.descriptionPublication status: aheadofprint
dc.description.abstractAllergic diseases are considered among the major burdons of public health with increased prevalence globally. Histamine H1-receptor antagonists are the foremost commonly used drugs in the treatment of allergic disorders. Our target drug is one of this class, loratadine and its biometabolite desloratadine which is also a non sedating H1 receptor antagonist with anti-histaminic action of 2.5 to 4 times greater than loratadine. To develop and validate a novel isocratic reversed-phase high performance liquid chromatography (RP-HPLC) method for rapid and simultaneous separation and determination of loratadine and its metabolite, desloratadine in human plasma. The drug extraction method from plasma was based on protein precipitation technique. The separation was carried out on a Thermo Scientific BDS Hypersil C18 column (5µm, 250 x 4.60 mm) using a mobile phase of MeOH : 0.025M KH2PO4 adjusted to pH 3.50 using orthophosphoric acid (85 : 15, v/v) at ambient temperature. The flow rate was maintained at 1 mL/min and maximum absorption was measured using PDA detector at 248 nm. The retention times of loratadine and desloratadine in plasma samples were recorded to be 4.10 and 5.08 minutes respectively, indicating a short analysis time. Limits of detection were found to be 1.80 and 1.97 ng/mL for loratadine and desloratadine, respectively, showing a high degree of method sensitivity. The method was then validated according to FDA guidelines for the determination of the two analytes in human plasma. The results obtained indicate that the proposed method is rapid, sensitive in the nanogram range, accurate, selective, robust and reproducible compared to other reported methods. [Abstract copyright: Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.]
dc.languageeng
dc.sourceeissn: 1875-5453
dc.subjectDesloratadine
dc.subjectFDA guidelines
dc.subjectHuman plasma
dc.subjectLoratadine
dc.subjectProtein precipitation
dc.subjectRP-HPLC
dc.titleDeveloping A High-performance Liquid Chromatography Method for Simultaneous Determination of Loratadine and its Metabolite Desloratadine in Human Plasma.
dc.typearticle
dc.date.updated2019-12-11T01:56:36Z
dc.date.accepted2019-09-05


This item appears in the following Collection(s)

Show simple item record