A novel method to optimise the utility of underused moulted plumulaceous feather samples for genetic analysis in bird conservation.
dc.contributor.author | Peters, Catherine | |
dc.contributor.author | Nelson, Howard | |
dc.contributor.author | Rusk, Bonnie | |
dc.contributor.author | Muir, Anna | |
dc.date.accessioned | 2019-10-30T12:21:06Z | |
dc.date.available | 2019-10-30T12:21:06Z | |
dc.date.issued | 2019-10-24 | |
dc.identifier.citation | Peters, C., Nelson, H. P., Rusk, B., Muir A. P. (2019) A novel method to optimise the utility of underused moulted plumulaceous feather samples for genetic analysis in bird conservation. Conservation Genetic Resources, 12, 457–467 | en_US |
dc.identifier.issn | 1877-7252 | |
dc.identifier.doi | 10.1007/s12686-019-01117-8 | |
dc.identifier.uri | http://hdl.handle.net/10034/622766 | |
dc.description | This is a post-peer-review, pre-copyedit version of an article published in Conservation Genetics Resources. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12686-019-01117-8 | |
dc.description.abstract | Non-invasive sampling methods are increasingly being used in conservation research as they reduce or eliminate the stress and disturbance resulting from invasive sampling of blood or tissue. Here we present a protocol optimised for obtaining usable genetic material from moulted plumulaceous feather samples. The combination of simple alterations to a ‘user-developed’ method, comprised of increased incubation time and modification of temperature and volume of DNA elution buffer, are outlined to increase DNA yield and significantly increase DNA concentration (W = 81, p <0.01, Cohens’s d= 0.89). We also demonstrate that the use of a primerless Polymerase Chain Reaction (PCR) technique increases DNA quality and amplification success when used prior to PCR reactions targeting avian mitochondrial DNA (mtDNA). A small amplicon strategy proved effective for mtDNA amplification using PCR, targeting three overlapping 314-359bp regions of the cytochrome oxidase I barcoding region which, when combined, aligned with target-species reference sequences. We provide evidence that samples collected non-invasively in the field and kept in non-optimal conditions for DNA extraction can be used effectively to sequence a 650bp region of mtDNA for genetic analysis. | en_US |
dc.publisher | Springer | en_US |
dc.relation.url | https://link.springer.com/journal/12686 | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | Non-invasive sampling | en_US |
dc.subject | Feather samples | en_US |
dc.subject | Polymerase Chain Reaction (PCR) | en_US |
dc.subject | Primerless PCR | en_US |
dc.subject | mtDNA | en_US |
dc.subject | Small amplicon strategy | en_US |
dc.title | A novel method to optimise the utility of underused moulted plumulaceous feather samples for genetic analysis in bird conservation. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 1877-7260 | en_US |
dc.contributor.department | University of Chester | en_US |
dc.identifier.journal | Conservation Genetic Resources | en_US |
or.grant.openaccess | Yes | en_US |
rioxxterms.funder | Unfunded | en_US |
rioxxterms.identifier.project | Unfunded | en_US |
rioxxterms.version | AM | en_US |
rioxxterms.versionofrecord | https://doi.org/10.1007/s12686-019-01117-8 | |
rioxxterms.licenseref.startdate | 2020-10-24 | |
rioxxterms.publicationdate | 2019-10-24 | |
dc.dateAccepted | 2019-10-16 | |
dc.date.deposited | 2019-10-30 | en_US |
dc.indentifier.issn | 1877-7252 | en_US |