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dc.contributor.authorPeters, Catherine
dc.contributor.authorNelson, Howard
dc.contributor.authorRusk, Bonnie
dc.contributor.authorMuir, Anna
dc.date.accessioned2019-10-30T12:21:06Z
dc.date.available2019-10-30T12:21:06Z
dc.date.issued2019-10-24
dc.identifier.citationPeters, C., Nelson, H. P., Rusk, B., Muir A. P. (2019) A novel method to optimise the utility of underused moulted plumulaceous feather samples for genetic analysis in bird conservation. Conservation Genetic Resources, 12, 457–467en_US
dc.identifier.issn1877-7252
dc.identifier.doi10.1007/s12686-019-01117-8
dc.identifier.urihttp://hdl.handle.net/10034/622766
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Conservation Genetics Resources. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12686-019-01117-8
dc.description.abstractNon-invasive sampling methods are increasingly being used in conservation research as they reduce or eliminate the stress and disturbance resulting from invasive sampling of blood or tissue. Here we present a protocol optimised for obtaining usable genetic material from moulted plumulaceous feather samples. The combination of simple alterations to a ‘user-developed’ method, comprised of increased incubation time and modification of temperature and volume of DNA elution buffer, are outlined to increase DNA yield and significantly increase DNA concentration (W = 81, p <0.01, Cohens’s d= 0.89). We also demonstrate that the use of a primerless Polymerase Chain Reaction (PCR) technique increases DNA quality and amplification success when used prior to PCR reactions targeting avian mitochondrial DNA (mtDNA). A small amplicon strategy proved effective for mtDNA amplification using PCR, targeting three overlapping 314-359bp regions of the cytochrome oxidase I barcoding region which, when combined, aligned with target-species reference sequences. We provide evidence that samples collected non-invasively in the field and kept in non-optimal conditions for DNA extraction can be used effectively to sequence a 650bp region of mtDNA for genetic analysis.en_US
dc.publisherSpringeren_US
dc.relation.urlhttps://link.springer.com/journal/12686en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectNon-invasive samplingen_US
dc.subjectFeather samplesen_US
dc.subjectPolymerase Chain Reaction (PCR)en_US
dc.subjectPrimerless PCRen_US
dc.subjectmtDNAen_US
dc.subjectSmall amplicon strategyen_US
dc.titleA novel method to optimise the utility of underused moulted plumulaceous feather samples for genetic analysis in bird conservation.en_US
dc.typeArticleen_US
dc.identifier.eissn1877-7260en_US
dc.contributor.departmentUniversity of Chesteren_US
dc.identifier.journalConservation Genetic Resourcesen_US
or.grant.openaccessYesen_US
rioxxterms.funderUnfundeden_US
rioxxterms.identifier.projectUnfundeden_US
rioxxterms.versionAMen_US
rioxxterms.versionofrecordhttps://doi.org/10.1007/s12686-019-01117-8
rioxxterms.licenseref.startdate2020-10-24
rioxxterms.publicationdate2019-10-24
dc.dateAccepted2019-10-16
dc.date.deposited2019-10-30en_US
dc.indentifier.issn1877-7252en_US


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