CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells.
Al-Delfi, Ibtesam R T
Johnson, William E B
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AbstractCD271 is a marker of bone marrow MSCs with enhanced differentiation capacity for bone or cartilage repair. However, the nature of CD271+ MSCs from adipose tissue (AT) is less well understood. Here, we investigated the differentiation, wound healing and angiogenic capacity of plastic adherent MSCs (PA MSCs) versus CD271+ MSCs from AT. There was no difference in the extent to which PA MSCs and CD271+ MSCs formed osteoblasts, adipocytes or chondrocytes in vitro. In contrast, CD271+ MSCs transplanted into athymic rats significantly enhanced osteochondral wound healing with reduced vascularisation in the repair tissue compared to PA MSCs and control animals; there was little histological evidence of mature articular cartilage formation in all animals. Conditioned medium from CD271+ MSC cultures was less angiogenic than PA MSC conditioned medium, and had little effect on endothelial cell migration or endothelial tubule formation in vitro. The low angiogenic activity of CD271+ MSCs and improved early stage tissue repair of osteochondral lesions when transplanted, along with a comparable differentiation capacity along mesenchymal lineages when induced, suggests that these selected cells are a better candidate than PA MSCs for the repair of cartilaginous tissue.
CitationKohli, N., Al-Delfi, I.R.T., Snow, M., Sakamoto, T., Miyazaki, T., Nakajima, H., Uchida, K, & Johnson, W.E.B. (2019). CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic then plastic adherent mesenchymal stem cells. Scientific Reports, 9(1),3194
DescriptionFunder: RCUK | Biotechnology and Biological Sciences Research Council (BBSRC); Grant(s): BB/H016570/1