Enhanced growth-inhibitory effect of microemulsified curcumin formulation in human prostate cancer LNCaP Cells
Affiliation
University of Chester, University of UlsterPublication Date
2015-01-01
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Aim: To assess the effect of curcumin microemulsified with non-ionic surfactant surfynol 465 W or dispersed using edible oils on prostate LNCaP cancer cell viability and glutathione status. Methodology: LNCaP cells were treated for 72-144 hr with curcumin dissolved with fish or corn oil and microemulsified using non-ionic surfactant surfynol 465 W; alternatively LNCaP cells were treated with curcumin directly dispersed in fish or corn oil (0-50 μM) for 24 -72-144 hr. Cell viability was determined using resazurin (Vision blueTM) fluorescence assay. Glutathione status was determined by monochlorobimane (MCB) assay. Results: Treatment with 0-34 μM of microemulsified curcumin produced moderate cytotoxic effect on LNCaP cells, no 50% reduction of cell viability was observed graphically. However, when LNCaP cells were treated with curcumin dispersed with corn oil the concentration or 50% reduction of cell viability (IC50) was 12-45 μM. Similarly for cells treated with curcumin dispersed with fish oil, the IC50 was between 20-40 μM. Cytotoxic doses of curcumin dispersed with corn or fish oil increased GST status in cells by 272-656% (p =<0.01). Conclusion: Microemulsified curcumin formulation prepared using fish or corn oil and surfynol 465 W surfactant had an inhibitory effect on viability of LNCaP cells as did direct dispersion of curcumin in fish or corn oil coupled with the ability for inducing intracellular GST status in LNCaP cells.Citation
Dubey, V., & Owusu-Apenten, R. (2015). Enhanced growth-inhibitory effect of microemulsified curcumin formulation in human prostate cancer LNCaP Cells. British Journal of Pharmaceutical Research, 5(3), 209-216.Publisher
SCIENCEDOMAIN InternationalAdditional Links
http://www.sciencedomain.org/abstract/7465Type
ArticleLanguage
enISSN
2231-2919EISSN
2231-2919ae974a485f413a2113503eed53cd6c53
10.9734/BJPR/2015/14475
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