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dc.contributor.authorBarlow, R.*
dc.contributor.authorBarnes, D. A.*
dc.contributor.authorCampbell, Anna M.*
dc.contributor.authorNigam, Poonam S.*
dc.contributor.authorOwusu-Apenten, Richard K.*
dc.date.accessioned2019-02-25T13:41:32Z
dc.date.available2019-02-25T13:41:32Z
dc.date.issued2015-11-10
dc.identifier.citationBarlow, R., Barnes, D., Campbell, A., Nigam, P. S., & Owusu-Apenten, R. (2015). Antioxidant, anticancer and antimicrobial, effects of Rubia cordifolia aqueous root extract. Journal of Advances in Biology & Biotechnology, 5(1), 6-14.
dc.identifier.doi10.9734/JABB/2016/22625
dc.identifier.urihttp://hdl.handle.net/10034/621907
dc.descriptionReceived 15th October 2015 Accepted 29th October 2015 Published 10th November 2015
dc.description.abstractAims: To evaluate the total antioxidant capacity (TAC) of Rubia cordifolia root extracts, to test anticancer activity against MDA-MB-231 breast cancer cell lines, and to evaluate antimicrobial activity of the same extract versus six Gram-positive and negative bacteria. Study Design: In vitro. Place of Study and Duration: School of Biomedical Sciences, Ulster University, July 2014-Sept 2015. Methodology: TAC was tested using ABTS, DPPH, FRAP and Folin assays and values were expressed as mg-gallic acid equivalents per 100 g (GAE/100 g) of sample. Anticancer properties were examined against MDA-MB-231 breast cancer cell lines using Sulforhodamine B assay. Antimicrobial activity was examined using a disk diffusion assay with three Gram-positive (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi) bacteria. Results: TAC of dry extracts of Rubia cordifolia ranged from 523±43 to 4513±208 (mg GAE mg/100 g) depending on the method of analysis, ABTS> FRAP> Folin > DPPH methods. R. cordifolia dry extract showed cytotoxicity against MDA-MB-231 with IC50 = 44 µg/ml or 5.1µM GAE. No antimicrobial activity was observed against the three Gram-positive, or three Gram-negative bacterial species using the water extract or R. cordifolia. Conclusion: R. cordifolia aqueous extract possess high total antioxidant capacity but values depend on the method of analysis. R. cordifolia extract inhibits MDA-MB-231 breast cancer cells proliferation but nil anti-bacterial activity was observed for three Gram-positive and three Gram-negative bacterial strains tested.
dc.language.isoenen
dc.publisherSciencedomain internationalen_US
dc.relation.urlhttp://www.sciencedomain.org/abstract/12260en_US
dc.rightsAttribution-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/en_US
dc.subjectManjisthaen_US
dc.subjectRubia cordifoliaen_US
dc.subjectantioxidanten_US
dc.subjectantimicrobialen_US
dc.subjectMDA-MB-231en_US
dc.subjectbreast canceren_US
dc.subjectABTSen_US
dc.subjectDPPHen_US
dc.subjectFRAPen_US
dc.titleAntioxidant, Anticancer and Antimicrobial, Effects of Rubia cordifolia Aqueous Root Extracten_US
dc.typeArticleen_US
dc.identifier.eissn2394-1081
dc.contributor.departmentUniversity of Chester, University of Ulster
dc.identifier.journalJournal of Advances in Biology & Biotechnologyen_US
dc.date.accepted2015-10-29
or.grant.openaccessYesen_US
rioxxterms.funderNAen_US
rioxxterms.identifier.projectNAen_US
rioxxterms.versionVoRen_US
rioxxterms.licenseref.startdate2015-11-10


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Except where otherwise noted, this item's license is described as Attribution-NoDerivatives 4.0 International