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dc.contributor.authorOwusu-Apenten, Richard K.*
dc.contributor.authorKelly, Theresa*
dc.date.accessioned2019-02-12T15:47:15Z
dc.date.available2019-02-12T15:47:15Z
dc.date.issued2015-03-24
dc.identifier.citationKelly, T., & Owusu-Apenten, R. K. (2015). Effect of methotrexate and tea polyphenols on the viability and oxidative stress in MDA-MB-231 breast cancer cells. J Appl Life Sci Int, 2(4), 152-9.
dc.identifier.doi10.9734/JALSI/2015/14142
dc.identifier.urihttp://hdl.handle.net/10034/621860
dc.description.abstractAim: To determine the effect of tea polyphenols and methotrexate on viability and reactive oxygen species (ROS) in a naturally resistant breast cancer cell line MDA-MB-231. Methodology: MDA-MB-231 cells were selected as a model for methotrexate resistant breast cancer. Drug tests were performed over 72 hours at concentrations 0-100 µM. Pre-treatments were with quercetin (QE) or epigallocatechin gallate (EGCG) for 5 hours followed by methotrexate. Cytotoxicity was measured using the MTT assay or resazurin fluorescence assay. ROS was determined using the 2’, 7’-dichlorofluorescein diacetate assay. Intracellular GSH was measured using the monochlorobimane assay. Results: Methotrexate was cytotoxic to MDA-MB-231 cells with IC50 of 35±4 µM. The IC50 value was 68±9.4 µM with QE and 83±16 µM for EGCG. The pre-treatment with QE and EGCG lowered the IC50 for methotrexate by 28% (P =0.009) and 16% (P=0.2027). Intracellular ROS concentrations increased after treatment with methotrexate, QE or EGCG singly and ROS decreased with combination treatment compared with the response for methotrexate only. There were no significant changes in intracellular GSH. Conclusion: Pretreatment with tea polyphenols partially sensitized breast cancer cells towards methotrexate and decreases intracellular ROS. More research is needed to optimize the sensitizing effect of tea phenols on the breast cancer cell response to methotrexate.
dc.language.isoenen
dc.publisherSCIENCEDOMAIN Internationalen_US
dc.relation.urlhttp://www.sciencedomain.org/abstract/8571en_US
dc.rightsAttribution-NoDerivatives 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectbreast canceren_US
dc.subjectmethotrexateen_US
dc.subjectquercetinen_US
dc.subjectepigallocatechin gallateen_US
dc.subjectoxidative stressen_US
dc.subjectanticancer effecten_US
dc.titleEffect of Methotrexate and Tea Polyphenols on the Viability and Oxidative Stress in MDA-MB-231 Breast Cancer Cellsen_US
dc.typeArticleen_US
dc.identifier.eissn2394-1103
dc.contributor.departmentUniversity of Ulster
dc.identifier.journalJournal of Applied Life Sciences Internationalen_US
dc.date.accepted2015-03-11
or.grant.openaccessYesen_US
rioxxterms.funderunfundeden_US
rioxxterms.identifier.projectunfundeden_US
rioxxterms.versionVoRen_US
rioxxterms.licenseref.startdate2019-02-12
refterms.dateFCD2019-02-08T16:17:09Z
refterms.versionFCDEVoR
refterms.dateFOA2019-02-12T15:47:15Z


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