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dc.contributor.authorChau, Tsz Ching
dc.contributor.authorOwusu-Apenten, Richard
dc.contributor.authorNigam, Poonam
dc.date.accessioned2018-08-23T10:55:00Z
dc.date.available2018-08-23T10:55:00Z
dc.date.issued2017-10-28
dc.identifier.citationChau, T. C., Owusu-Apenten, R., & Nigam, P. S. (2017). Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract. J Adv Biol Biotech, 15, 1-6.en_US
dc.identifier.doi10.9734/JABB/2017/37101
dc.identifier.urihttp://hdl.handle.net/10034/621361
dc.description.abstractAims: To evaluate total phenols content (TPC), antioxidant capacity (TAC) and antibacterial activity of Manuka honey extract (MHE) and to compare such properties with those for unfractionated Manuka honey. Study Design: In vitro study. Place and Duration of Study: School of Biomedical Sciences, Ulster University, Coleraine, UK. Between September 2016 and September 2017. Methodology: MHE was prepared by solvent extraction using ethyl acetate. TPC was determined by Folin-Ciocalteu assay. The iron (III) reducing antioxidant capacity (IRAC) method was used to determine TAC. Antibacterial activity was evaluated using disc diffusion assay and 96-well microtiter plate methods with absorbance measured at 600 nm. Results: The TPC for MHE was 30-fold higher than the value for Manuka honey (33420±1685 mg vs. 1018±78 mg GAE/kg) while TAC values were~ 100-times greater (83,198±7064 vs. 793±104 TEAC, respectively). Antibacterial activity assessed by disc diffusion for Manuka honey (18.5 mm on S. aureus and 20 mm on E. coli) was two times greater than for MHE (9mm for both S. aureus and E. coli). The 96-well microtiter plate assay confirmed the greater antibacterial activity for Manukaen_US
dc.language.isoenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.urlhttp://www.sciencedomain.org/abstract/21616en_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/en_US
dc.subjectManuka honeyen_US
dc.subjectmanuka extracten_US
dc.subjecttotal phenolsen_US
dc.subjectantioxidanten_US
dc.subjectAntibacterialen_US
dc.titleTotal Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract.en_US
dc.typeArticleen_US
dc.identifier.eissn2394-1081
dc.contributor.departmentUlster University; University of Chester;en_US
dc.identifier.journalJournal of Advances in Biology & Biotechnologyen_US
dc.date.accepted2017-10-20
or.grant.openaccessYesen_US
rioxxterms.funderunfundeden_US
rioxxterms.identifier.projectunfundeden_US
rioxxterms.versionVoRen_US
refterms.dateFCD2018-08-10T21:24:24Z
refterms.versionFCDP
refterms.dateFOA2018-08-23T10:55:00Z


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