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dc.contributor.authorHu, Bing*
dc.contributor.authorHan, Lingyu*
dc.contributor.authorGao, Zhiming*
dc.contributor.authorZhang, Ke*
dc.contributor.authorAl-Assaf, Saphwan*
dc.contributor.authorNishinari, Katsuyoshi*
dc.contributor.authorPhillips, Glyn O.*
dc.contributor.authorYang, Jixin*
dc.contributor.authorFang, Yapeng*
dc.date.accessioned2018-06-19T11:08:28Z
dc.date.available2018-06-19T11:08:28Z
dc.date.issued2018-05-14
dc.identifier.citationHu, B., Han, L., Gao, Z., Zhang, K., Al-Assaf, S., Nishinari, K., . . . Fang, Y. (2018). Effects of temperature and solvent condition on phase separation induced molecular fractionation of gum arabic/hyaluronan aqueous mixtures. International Journal of Biological Macromolecules, 116, 683-690. https://doi.org/10.1016/j.ijbiomac.2018.05.073
dc.identifier.doi10.1016/j.ijbiomac.2018.05.073
dc.identifier.urihttp://hdl.handle.net/10034/621201
dc.description.abstractEffects of temperature and solvent condition on phase separation-induced molecular fractionation of gum arabic/hyaluronan (GA/HA) mixed solutions were investigated. Two gum arabic samples (EM10 and STD) with different molecular weights and polydispersity indices were used. Phase diagrams, including cloud and binodal curves, were established by visual observation and GPC-RI methods. The molecular parameters of control and fractionated GA, from upper and bottom phases, were measured by GPC-MALLS. Fractionation of GA increased the content of arabinogalactan-protein complex (AGP) from ca. 11% to 18% in STD/HA system and 28% to 55% in EM10/HA system. The phase separation-induced molecular fractionation was further studied as a function of temperature and solvent condition (varying ionic strength and ethanol content). Increasing salt concentration (from 0.5 to 5 mol/L) greatly reduced the extent of phase separation-induced fractionation. This effect may be ascribed to changes in the degree of ionization and shielding of the acid groups. Increasing temperature (from 4oC to 80oC) also exerted a significant influence on phase separation-induced fractionation. The best temperature for GA/HA mixture system was 40oC while higher temperature negatively affected the fractionation due to denaturation and possibly degradation in mixed solutions. Increasing the ethanol content up to 30% showed almost no effect on the phase separation induced fractionation.
dc.language.isoenen
dc.publisherElsevier
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S014181301734641Xen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectPhase separationen
dc.subjectGum Arabicen
dc.subjectSolvent conditionen
dc.titleEffects of temperature and solvent condition on phase separation induced molecular fractionation of gum arabic/hyaluronan aqueous mixturesen
dc.typeArticleen
dc.identifier.eissn1879-0003
dc.contributor.departmentHubei University of Technology; Wrexham Glyndwr University
dc.identifier.journalInternational Journal of Biological Macromoleculesen
dc.date.accepted2018-05-12
or.grant.openaccessYesen
rioxxterms.funderUnfundeden
rioxxterms.identifier.projectUnfundeden
rioxxterms.versionAMen
rioxxterms.licenseref.startdate2019-05-14
html.description.abstractEffects of temperature and solvent condition on phase separation-induced molecular fractionation of gum arabic/hyaluronan (GA/HA) mixed solutions were investigated. Two gum arabic samples (EM10 and STD) with different molecular weights and polydispersity indices were used. Phase diagrams, including cloud and binodal curves, were established by visual observation and GPC-RI methods. The molecular parameters of control and fractionated GA, from upper and bottom phases, were measured by GPC-MALLS. Fractionation of GA increased the content of arabinogalactan-protein complex (AGP) from ca. 11% to 18% in STD/HA system and 28% to 55% in EM10/HA system. The phase separation-induced molecular fractionation was further studied as a function of temperature and solvent condition (varying ionic strength and ethanol content). Increasing salt concentration (from 0.5 to 5 mol/L) greatly reduced the extent of phase separation-induced fractionation. This effect may be ascribed to changes in the degree of ionization and shielding of the acid groups. Increasing temperature (from 4oC to 80oC) also exerted a significant influence on phase separation-induced fractionation. The best temperature for GA/HA mixture system was 40oC while higher temperature negatively affected the fractionation due to denaturation and possibly degradation in mixed solutions. Increasing the ethanol content up to 30% showed almost no effect on the phase separation induced fractionation.


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