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dc.contributor.authorZhang, Zhengxiao*
dc.contributor.authorSmith, Christopher J.*
dc.contributor.authorLi, Weili*
dc.contributor.authorAshworth, Jason J.*
dc.date.accessioned2018-02-23T16:32:46Z
dc.date.available2018-02-23T16:32:46Z
dc.date.issued2016-10-10
dc.identifier.citationZhang, Z., Smith, C. J., Li, W., & Ashworth, J. J. (2016). Characterization of Nitric Oxide Modulatory Activities of Alkaline-Extracted and Enzymatic-Modified Arabinoxylans from Corn Bran in Cultured Human Monocytes. Journal of Agricultural and Food Chemistry, 64(43), 8128–8137. DOI: 10.1021/acs.jafc.6b02896
dc.identifier.doi10.1021/acs.jafc.6b02896
dc.identifier.urihttp://hdl.handle.net/10034/620878
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.6b02896
dc.description.abstractThe ingestion of foods and food-derived substances that may mediate the immune system is widely studied. Evidence suggests cereal arabinoxylans (AXs) have immunomodulatory activities that may impart health benefits in terms of immune enhancement. This study extracted AXs from corn bran using alkali and developed a modification process using three endoxylanases to obtain fractions of lower molecular weight ranges. In vitro studies showed extracted and modified AXs significantly (P < 0.05) elevated nitric oxide (NO) synthesis by the human U937 monocytic cell line (ranging from 53.7 ± 1.1 to 62.9 ± 1.2 μM per million viable cells) at all concentrations tested (5–1000 μg/mL), indicative of immune enhancement compared to an untreated control (43.7 ± 1.9 μM per million viable cells). The study suggested the dose range and Mw distribution of AXs are key determinants of immune-modulatory activity. AXs in the low Mw range (0.1–10 KDa) were the most effective at inducing NO secretion by U937 macrophages at low AX concentration ranges (5–50 μg/mL), with NO production peaking at 62.9 ± 1.2 μM per million viable cells with 5 μg/mL of AX (P = 0.0009). In contrast, AXs in the high Mw range (100–794 kDa) were most effective at inducing NO at high AX concentration ranges (500–1000 μg/mL) with NO production reaching a maximum of 62.7 ± 1.3 μM per million viable cells at 1000 μg/mL of AX (P = 0.0011). The findings suggest that dietary AXs from corn bran may heighten innate immune responses in the absence of infection or disease.
dc.language.isoenen
dc.publisherAmerican Chemical Society
dc.relation.urlhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.6b02896en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectArabinoxylanen
dc.subjectCorn branen
dc.subjectimmune-modulatory activityen
dc.subjectIn vitroen
dc.subjectMolecular weighten
dc.titleCharacterization of Nitric Oxide Modulatory Activities of Alkaline-Extracted and Enzymatic-Modified Arabinoxylans from Corn Bran in Cultured Human Monocytesen
dc.typeArticleen
dc.identifier.eissn1520-5118
dc.contributor.departmentManchester Metropolitan University; University of Chester
dc.identifier.journalJournal of Agricultural and Food Chemistryen
dc.date.accepted2016-10-01
or.grant.openaccessYesen
rioxxterms.funderManchester Metropolitan Universityen
rioxxterms.identifier.projectL-30073en
rioxxterms.versionAMen
rioxxterms.licenseref.startdate2017-10-10
html.description.abstractThe ingestion of foods and food-derived substances that may mediate the immune system is widely studied. Evidence suggests cereal arabinoxylans (AXs) have immunomodulatory activities that may impart health benefits in terms of immune enhancement. This study extracted AXs from corn bran using alkali and developed a modification process using three endoxylanases to obtain fractions of lower molecular weight ranges. In vitro studies showed extracted and modified AXs significantly (P < 0.05) elevated nitric oxide (NO) synthesis by the human U937 monocytic cell line (ranging from 53.7 ± 1.1 to 62.9 ± 1.2 μM per million viable cells) at all concentrations tested (5–1000 μg/mL), indicative of immune enhancement compared to an untreated control (43.7 ± 1.9 μM per million viable cells). The study suggested the dose range and Mw distribution of AXs are key determinants of immune-modulatory activity. AXs in the low Mw range (0.1–10 KDa) were the most effective at inducing NO secretion by U937 macrophages at low AX concentration ranges (5–50 μg/mL), with NO production peaking at 62.9 ± 1.2 μM per million viable cells with 5 μg/mL of AX (P = 0.0009). In contrast, AXs in the high Mw range (100–794 kDa) were most effective at inducing NO at high AX concentration ranges (500–1000 μg/mL) with NO production reaching a maximum of 62.7 ± 1.3 μM per million viable cells at 1000 μg/mL of AX (P = 0.0011). The findings suggest that dietary AXs from corn bran may heighten innate immune responses in the absence of infection or disease.


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