Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes

Pickard, Mark R.; Adams, Christopher F.; Chari, Divya M.

dc.contributor.author	Pickard, Mark R.	*
dc.contributor.author	Adams, Christopher F.	*
dc.contributor.author	Chari, Divya M.	*
dc.date.accessioned	2017-04-07T11:17:29Z
dc.date.available	2017-04-07T11:17:29Z
dc.date.issued	2017-02-02
dc.identifier.citation	Pickard, M. R., Adams, C. F., & Chari, D. M. (2017). Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes. Current Protocols in Stem Cell Biology, 40:2D.19.1-2D.19.16. DOI: 10.1002/cpsc.23
dc.identifier.isbn	9780470151808
dc.identifier.doi	10.1002/cpsc.23
dc.identifier.uri	http://hdl.handle.net/10034/620474
dc.description	This is the peer reviewed version of the following article: Pickard, M. R., Adams, C. F., & Chari, D. M. (2017). Magnetic Nanoparticle‐Mediated Gene Delivery to Two‐ and Three‐Dimensional Neural Stem Cell Cultures: Magnet‐Assisted Transfection and Multifection Approaches to Enhance Outcomes, Current Protocols in Stem Cell Biology, 40(1), 2D.19.1-2D.19.16, which has been published in final form athttps://doi.org/10.1002/cpsc.23 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
dc.description.abstract	Neural stem cells (NSCs) have high translational potential in transplantation therapies for neural repair. Enhancement of their therapeutic capacity by genetic engineering is an important goal for regenerative neurology. Magnetic nanoparticles (MNPs) are major non-viral vectors for safe bioengineering of NSCs, offering critical translational benefits over viral vectors, including safety, scalability, and ease of use. This unit describes protocols for the production of suspension (neurosphere) and adherent (monolayer) murine NSC cultures. Genetic engineering of NSCs with MNPs and the application of 'magnetofection' (magnetic fields) or 'multifection' (repeat transfection) approaches to enhance gene delivery are described. Magnetofection of monolayer cultures achieves optimal transfection, but neurospheres offer key advantages for neural graft survival post-transplantation. A protocol is presented which allows the advantageous features of each approach to be combined into a single procedure for transplantation. The adaptation of these protocols for other MNP preparations is considered, with emphasis on the evaluation of procedural safety.
dc.language.iso	en	en
dc.publisher	Wiley
dc.relation.url	http://onlinelibrary.wiley.com/doi/10.1002/cpsc.23/abstract	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	en
dc.subject	Magnetic nanoparticles	en
dc.subject	Neural stem cells	en
dc.subject	Gene delivery	en
dc.subject	Magnetofection	en
dc.subject	Transplantation	en
dc.title	Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes	en
dc.type	Article	en
dc.identifier.eissn	1938-8969
dc.contributor.department	University of Chester; Keele University
dc.identifier.journal	Current Protocols in Stem Cell Biology	en
dc.date.accepted	2016-11-02
or.grant.openaccess	Yes	en
rioxxterms.funder	Unfunded	en
rioxxterms.identifier.project	Unfunded	en
rioxxterms.version	AM	en
rioxxterms.licenseref.startdate	2019-08-13
html.description.abstract	Neural stem cells (NSCs) have high translational potential in transplantation therapies for neural repair. Enhancement of their therapeutic capacity by genetic engineering is an important goal for regenerative neurology. Magnetic nanoparticles (MNPs) are major non-viral vectors for safe bioengineering of NSCs, offering critical translational benefits over viral vectors, including safety, scalability, and ease of use. This unit describes protocols for the production of suspension (neurosphere) and adherent (monolayer) murine NSC cultures. Genetic engineering of NSCs with MNPs and the application of 'magnetofection' (magnetic fields) or 'multifection' (repeat transfection) approaches to enhance gene delivery are described. Magnetofection of monolayer cultures achieves optimal transfection, but neurospheres offer key advantages for neural graft survival post-transplantation. A protocol is presented which allows the advantageous features of each approach to be combined into a single procedure for transplantation. The adaptation of these protocols for other MNP preparations is considered, with emphasis on the evaluation of procedural safety.