Now showing items 1-20 of 679

    • Communities and neighbourhoods

      Vaandrager, Lenneke; Kennedy, Lynne; Wageningen University and University of Chester (Springer, 2016-09-07)
      Summary There is growing consensus that the places where people live and the various social processes, relationships and psycho-social concepts associated with strong healthy communities and neighbourhoods make an important contribution to health. Where you live makes a considerable difference; people living in more affluent communities for example are more likely to experience better self-reported health and wellbeing. This is particularly evident in current theoretical and policy debates concerning the salutogenic and so-called strength or assets based approach to health; healthy communities have various social and physical resources available, which if they can recognise, share and utilise, can result in stronger SOC, increasing their ability to cope and thrive. Within health promotion we actively encourage communities to organise themselves for better health and well-being. The concept of ‘community’ is both complex and subjective and difficult to define. So we start by conceptualising the definitions, dimensions and meanings of community – beyond a physical location - underpinning this chapter. There are several ideas linking the community or neighbourhood as a setting, including community as a place to live, connectedness (social capital) and social action (the development of a strong SOC). The evidence is variable in quality and furthermore, few studies explicitly apply the theory of salutogenesis when they study health and wellbeing in the community context. The body of this chapter is devoted therefore to summarising the available research about salutogenic and asset-based community interventions, drawing upon examples from empirical work. In doing so, we will highlight debates emerging around the concepts of a salutogenic framework and health assets in relation to community and neighbourhood. As such, we are specifically interested in examining the resources (and/or assets) of communities and neighbourhoods and the associated processes enabling these resources to be accessed for the benefit of the community’s health and wellbeing.
    • CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells.

      Kohli, Nupur; Al-Delfi, Ibtesam R T; Snow, Martyn; Sakamoto, Takumi; Miyazaki, Tsuyoshi; Nakajima, Hideaki; Uchida, Kenzo; Johnson, William E B (2019-02-28)
      CD271 is a marker of bone marrow MSCs with enhanced differentiation capacity for bone or cartilage repair. However, the nature of CD271+ MSCs from adipose tissue (AT) is less well understood. Here, we investigated the differentiation, wound healing and angiogenic capacity of plastic adherent MSCs (PA MSCs) versus CD271+ MSCs from AT. There was no difference in the extent to which PA MSCs and CD271+ MSCs formed osteoblasts, adipocytes or chondrocytes in vitro. In contrast, CD271+ MSCs transplanted into athymic rats significantly enhanced osteochondral wound healing with reduced vascularisation in the repair tissue compared to PA MSCs and control animals; there was little histological evidence of mature articular cartilage formation in all animals. Conditioned medium from CD271+ MSC cultures was less angiogenic than PA MSC conditioned medium, and had little effect on endothelial cell migration or endothelial tubule formation in vitro. The low angiogenic activity of CD271+ MSCs and improved early stage tissue repair of osteochondral lesions when transplanted, along with a comparable differentiation capacity along mesenchymal lineages when induced, suggests that these selected cells are a better candidate than PA MSCs for the repair of cartilaginous tissue.
    • In vitro cyto-toxic assessment of heavy metals and their binary mixtures on mast cell-like, rat basophilic leukemia (RBL-2H3) cells.

      ThankGod Eze, Chukwuebuka; email:; Michelangeli, Francesco; email:; Otitoloju, Adebayo Akeem; email: (2019-02-11)
      We investigated the cytotoxicity and mechanisms of cell death induced by salts of Cadmium (Cd ), Lead (Pb ), Arsenic (AsO ) and Chromium (Cr ) on RBL-2H3 cells (a model mast cell line). In addition, cyto-toxic effect on cell viability was assessed to reveal their nature of interaction in binary mixture. The individual cytotoxic characteristics of these metals on RBL-2H3 cell viability showed a concentration-dependent reduction of cell viability. We observed that concentration-dependent cytotoxic potency on RBL-2H3 cells of these metals range in the following order Cd >Cr >As O > Pb with LC values of 0.11 μM, 93.58 μM, 397.9 μM and 485.3 μM respectively. Additive effects were observed with Pb  + Cd , Pb  + AsO , Pb  + Cr and AsO  + Cr . The study revealed that Pb , Cd , AsO and Cr could induce significant (P < 0.01) cell death by apoptosis in RBL-2H3. Highly significant necrotic cell death was observed with Pb and Cr (P < 0.01) than Cd and AsO (P < 0.05). Overall, it can be deduced that several cell death executing pathways may be concomitantly activated on exposure to heavy metals and the predominance of one over others might depend on the type of heavy metal, concentration and the metabolic state of the cell. Eventually, binary mixtures of some of these metals showed less cytotoxicity than would be expected from their individual actions and may depend on the co-exposure of the metal ions and their modes of action. [Abstract copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.]
    • Isoform-specific Ras signaling is growth factor dependent

      Hood, Fiona; Klinger, Bertram; Newlaczyl, Anna; Sieber, Anja; Dorel, Mathurin; Oliver, Simon; Coulsen, Judy; Bluthgen, Nils; Prior, Ian; University of Liverpool; Universitätsmedizin Berlin; University of Chester (ASCB, 2019-02-20)
      HRAS, NRAS and KRAS isoforms are almost identical proteins that are ubiquitously expressed and activate a common set of effectors. In vivo studies have revealed that they are not biologically redundant; however, the isoform-specificity of Ras signaling remains poorly understood. Using a novel panel of isogenic SW48 cell lines endogenously expressing wild type or G12V mutated activated Ras isoforms we have performed a detailed characterization of endogenous isoform-specific mutant Ras signaling. We find that despite displaying significant Ras activation, the downstream outputs of oncogenic Ras mutants are minimal in the absence of growth factor inputs. The lack of mutant KRAS-induced effector activation observed in SW48 cells appears to be representative of a broad panel of colon cancer cell lines harboring mutant KRAS. For MAP kinase pathway activation in KRAS mutant cells, the requirement for co-incident growth factor stimulation occurs at an early point in the Raf activation cycle. Finally, we find that Ras isoform-specific signaling was highly context dependent and did not conform to the dogma derived from ectopic expression studies.
    • Integrating role-play with case study and carbon footprint monitoring: A transformative approach to enhancing learners' behaviour for a more sustainable environment

      Oliver, Simon; University of Chester (Look Acedemic Publishers, 2016-05-01)
      Educators have long used role-play to encourage a significant shift in learner perspective, and the social and cognitive benefits of this active learning tool are well documented. Although the use of role-play has been encouraged as a transformative approach to challenge the worldview of individual learners in the context of environmental education, the efficacy of its application as a direct means to enhance learners’ behavior towards the environment has not been previously investigated. In this study role-play simulations were integrated with case study to expose learners to some of the socioscientific issues that typically arise from environmental debate. Learners were separated into groups representing the interests of parties that typically negotiate environmental affairs in real world scenarios (conservationists, scientists, politicians, NGOs, stakeholders), and tasked with preparing role-play simulations using a variety of flipped learning techniques. Learners’ carbon footprints were monitored pre and post intervention, and CO2 emission scores were used as a proxy for their behaviour towards the environment. Five role-play simulations were run overall. These were integrated with case studies associated with fisheries issues, and climate change. Anonymous participant surveys indicated that learners responded positively to the intervention, and participants’ CO2 emission scores improved significantly (T1,59 = 2.723, p = 0.009). In the context of environmental sciences, learners that engage in the integrated role-play and case study approach may benefit from flipped learning techniques to prepare their simulations, and gain confidence from self-actualising moments of achievement when they realise an improvement in their environmental behaviour.
    • A FRAP Assay at pH 7 unveils Extra Antioxidant Activity from Green, Black, White and Rooibos Tea but not Apple Tea

      Owusu-Apenten, Richard K.; Wong, C. W.; Cheung, W. S. M.; Lau, Y. Y.; Bolanos de la Torre, A. A. S.; University of Chester, University of Ulster (Verizona publisher, 2015-06-26)
      Abstract Aim: Realization of a ferric reducing antioxidant power (FRAP) assay at neutral pH and re-evaluation of tea antioxidant activity for comparisons with the standard FRAP assay. Method: A FRAP assay at neutral pH utilized ferrozine (7.3-(2-Pyridyl)-5, 6-diphenyl-1, 2, 4-triazine-4’, 4’’-disulfonic acid;ferrozine) dye in conjunction with Tris-HCl buffer (0.1M. pH 7.0) with 280 µl of regent addition to 20 µl of tea infusions and absorbance measurements at 562 nm with a microplate reader. Results: The microplate ferrozine FRAP assay (mFzFRAP) gave linear calibrations for ascorbic acid, gallic acid, ammonium ferrous sulphate, (AFS), trolox, cysteine and glutathione (R2 = 0.998 -1.000) with molar absorptivity (measures of sensitivity) similar to literature values. The analytical precision was 5-7% and the minimum detectable concentrations (MDC) were 1.4- 2.8 µM (0.4-0.8 nanomoles). Discussion: Values for FRAP were higher at pH 7.0 compared to pH 4.0 for gallic acid, ascorbic acid, glutathione, and cysteine possibly due to their ionization at high pH. The assay sensitivity for AFS and trolox were unchanged at pH 4.0 and pH 7.0. When assayed at pH 7 the water infusions from green tea, black tea, white tea, and rooibos tea had 200-360% antioxidant activity normally observable at low pH. Conclusion: A FRAP assay at pH 7 unveils extra antioxidant activity for green, black, white and Rooibos teas compared to values from the standard TptzFRAP (pH 3.6) method. As a recommendation, the antioxidant activity of teas and other herbal preparations should be re-evaluated over a wide pH range.
    • Will Plan S put learned societies in jeopardy?

      Purton, Mary; Michelangeli, Francesco; Fésüs, László (Wiley, 2019-02-25)
    • Socio-economic causes of undernutrition

      Kennedy, Lynne; Woodall, Alison; University of Chester (John Wiley and Sons, 2018-01-26)
      In this chapter we explore the role of socio-economic factors in the development of under-nutrition in high-income countries, such as the UK, with particular reference to food access and nutrition inequality. For the purpose of this chapter we use the term under-nutrition to refer to the physiological effects of inadequate food supply resulting from the inability to access sufficient quantity and quality of food to meet recommended nutritional requirements; a situation otherwise termed food poverty or food insecurity (See Box 1 for definitions). In affluent societies, hunger and malnutrition coexist alongside obesity and diet-related diseases such as coronary heart disease and diabetes. Before the food system was industrialised in the mid-20th Century, people ate a basic, traditional diet of limited variety. Hunger and under nutrition was common. Today, food is both varied and widely available. Access to cheap, energy-dense and nutrient-poor food is linked with the so-called obesity epidemic and diseases of affluence. Despite this a growing number of people in societies such as the UK experience hunger or malnutrition because of limited access or availability to a nutritionally adequate diet (3, 4, and 5).
    • Effects of ascorbic acid, dehydroascorbic acid and methotrexate on breast cancer cell viability.

      Dosunmu, Yewande; Owusu-Apenten, Richard K.; University of Chester, University of Ulster (Sciencedomain international, 2017-10-28)
      Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate (MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS). Study Design: In-vitro method. Place and Duration of Study: Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom. September 2016-2017 Methodology: Cytotoxicity tests were performed with MTX (0.01- 1000 µmol/l) alone or in combination with AA or DHA, for 72 h. Cell viability was measured by 3-4,5 dimethylthiazol-2,5 diphenyl tetrazolium bromide (MTT) or Sulforhodamine B (SRB) assays. Intracellular ROS was measured by 2’,7’-dichlorofluroscein diacetate assay. Results: Treatments of MDA-MB231 cells with single agents, showed dose dependent response with 50% inhibition of cell viability (IC50) of 110.5-201.4 µmol/l (MTX), 2237-5703 µmol/l (AA) or 2474 µmol/l (DHA). Combination studies showed clear synergisms for MTX (~10 µmol/l) and DHA or AA (1100 µmol/l) but weak or no interactions at other concentrations. Three days combination treatment of DHA showed decrease of ROS, which was reversed by MTX (>10 µmol/l). Conclusions: Co-treatment of methotrexate with AA or DHA showed synergism (C1<1.0) and enhanced cytotoxicity of the anti-folate towards MDA-MB-231 breast cancer cells. Intracellular ROS decreased with AA and DHA treatment, which might be useful for reducing MTX-related oxidative stress.
    • Exercise, or exercise and diet for the management of polycystic ovary syndrome: a systematic review and meta-analysis.

      Kite, Chris; orcid: 0000-0003-1342-274X; Lahart, Ian M; orcid: 0000-0003-1079-2876; Afzal, Islam; orcid: 0000-0002-7961-7753; Broom, David R; orcid: 0000-0002-0305-937X; Randeva, Harpal; orcid: 0000-0001-9438-7506; Kyrou, Ioannis; orcid: 0000-0002-6997-3439; Brown, James E (2019-02-12)
      Typically, management of PCOS focuses on lifestyle changes (exercise and diet), aiming to alleviate symptoms, and lower the associated risk of type 2 diabetes and cardiovascular disease. Our objective was to analyse evidence on the effectiveness of exercise in the management of PCOS, when compared to (i) usual care, (ii) diet alone, and (iii) exercise combined with diet, and also exercise combined with diet, compared to (i) control or usual care and (ii) diet alone. Relevant databases were searched (June 2017) with no time limit for trial inclusion. Eligible trials employed a randomised or quasi-randomised design to measure the chronic effects of exercise, or exercise and diet in women with PCOS. Searches returned 2390 articles; of those, 27 papers from 18 trials were included. Results are presented as mean difference (MD) and 95% confidence intervals (95% CI). Compared with control, exercise had a statistical effect on change from baseline fasting insulin (MD - 2.44 μIU/mL, 95% CIs - 4.24 to - 0.64; very low-quality evidence), HOMA-IR (- 0.57, - 0.99 to - 0.14; very low-quality evidence), total cholesterol (- 5.88 mg/dL, - 9.92 to - 1.83; low-quality evidence), LDL cholesterol (- 7.39 mg/dL, - 9.83 to - 4.95; low-quality evidence), and triglycerides (- 4.78 mg/dL, - 7.52 to - 2.05; low-quality evidence). Exercise also improved VO max (3.84 ml/kg/min, 2.87 to 4.81), waist circumference (- 2.62 cm, - 4.13 to - 1.11), and body fat percentage (- 1.39%, - 2.61 to - 0.18) when compared with usual care. No effect was found for change value systolic/diastolic blood pressure, fasting glucose, HDL cholesterol (all low-quality evidence), or waist-to-hip ratio. Many favourable change score findings were supported by post-intervention value analyses: fasting insulin (- 2.11 μIU/mL, - 3.49 to - 0.73), total cholesterol (- 6.66 mg/dL, - 11.14 to - 2.17), LDL cholesterol (- 6.91 mg/dL, - 12.02 to - 1.80), and VO max (5.01 ml/kg/min, 3.48 to 6.54). Statistically lower BMI (- 1.02 kg/m , - 1.81 to - 0.23) and resting heart rate (- 3.26 beats/min - 4.93 to - 1.59) were also revealed in post-intervention analysis. Subgroup analyses revealed the greatest improvements in overweight/obese participants, and more outcomes improved when interventions were supervised, aerobic in nature, or of a shorter duration. Based on limited data, we found no differences for any outcome between the effects of exercise and diet combined, and diet alone. It was not possible to compare exercise vs diet or exercise and diet combined vs diet. Statistically beneficial effects of exercise were found for a range of metabolic, anthropometric, and cardiorespiratory fitness-related outcomes. However, caution should be adopted when interpreting these findings since many outcomes present modest effects and wide CIs, and statistical effects in many analyses are sensitive to the addition/removal of individual trials. Future work should focus on rigorously designed, well-reported trials that make comparisons involving both exercise and diet. This systematic review was prospectively registered on the Prospero International Prospective Register of Systematic Reviews ( CRD42017062576 ).
    • Risk and resilience: high stakes for sharks making transjurisdictional movements to use a conservation area.

      Oliver, Simon Peter; Grothues, Thomas; Williams, Amie; Cerna, Voltaire; Silvosa, Medel; Cases, Gary; Reed, Matthew; Christopher, Simon; University of Chester; Rutgers University Marine Field Station; Scotish National Heritage; The Thresher Shark Research and Conservation Project; Divelink Cebu; Evolution Dive Resort; Scubazoo (Elsevier, 2019-02)
      Oceanic sharks are vulnerable to overexploitation due to their life-history strategies, and declines in their populations are well documented. While it is clear that pelagic sharks are often subjected to uncontrolled fishing for their meat and valuable fins, a lack of empirical knowledge, and transboundary jurisdictional issues have stalled many initiatives to protect them in the wild. Alopiids, including pelagic thresher sharks, are important to Asian fisheries, but the extent to which they are exploited in the Philippines is unknown. We fitted 14 pelagic thresher sharks with acoustic tags, and monitored their fine scale lateral movements near a seamount in the Central Visayas where their regular occurrence is prized as a tourism enterprise. Pelagic thresher sharks used a specific corridor to move away from the seamount after early morning visits to cleaner wrasse. Long range dispersals occurred at a mean rate of 3.79 ± 1.43 km h-1 and were attributed to foraging behavior. Daily foraging expeditions led pelagic thresher sharks to potentially venture across the jurisdictional waters of five provincial territories even when they regularly returned to the seamount. Thresher sharks preferred specific locations on the seamount where they interact with cleaner fish. While the seamount offers cleaner associated services and refuge provision for these rare and elusive sharks, disconnects between habitual predator and prey localizations are likely to increase their vulnerability to pressure from large scale fisheries that operate in the area.
    • Effect of pH on the Radical Quenching Capacity of Tea Infusions Using the ABTS•+ Assay

      Chan, Yuk Man; Cheng, Nga Kwan; Nigam, Poonam Singh; Owusu-Apenten, Richard K.; University of Chester, University of Ulster (Sciencedomain International, 2016-06-22)
      Aims: The aims of this study were to assess the impact of pH on the free radical quenching activity of tea infusions using a modified 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay and three antioxidant compounds as reference. Study Design: In-vitro method. Place and Duration of Study: Faculty of Life and Health Science, School of Biomedical Sciences, Ulster University, UK. From Sept 2014 and May 2016. Methodology: Free radical quenching capacity of tea (Earl grey, black tea, Ceylon tea, & green tea) infusions were investigated using persulfate activated ABTS with acetate buffer (pH 4.5) or phosphate buffer saline (pH 7.0) as solvent. Tests were performed using 96-well microplates, 20 µl of sample and 280 µl of ABTS reagent, and calibrated using ascorbic acid, trolox or gallic acid as reference antioxidants. Results: Gallic acid free radical quenching was pH dependent and unsuitable as reference. The free radical quenching capacity of trolox and ascorbic acid was not significantly different at pH 4.5 and pH 7.0. The radical quenching capacity of tea infusions expressed as Trolox Equivalent Antioxidant Capacity (TEAC) or Ascorbic Acid Equivalent Antioxidant Capacity (AAEAC) was greater by 50-300% at pH 7 compared to pH 4.5. Conclusion: The modified ABTS assay is suitable for examining the influence of pH on free radical quenching ability of tea samples. Gallic acid was not a suitable reference compound. The radical quenching capacity of tea infusions increases with rising pH.
    • Enhanced growth-inhibitory effect of microemulsified curcumin formulation in human prostate cancer LNCaP Cells

      Dubey, Vaibhav; Owusu-Apenten, Richard K.; University of Chester, University of Ulster (Sciencedomain international, 2015-01-01)
      Aim: To assess the effect of curcumin microemulsified with non-ionic surfactant surfynol 465 W or dispersed using edible oils on prostate LNCaP cancer cell viability and glutathione status. Methodology: LNCaP cells were treated for 72-144 hr with curcumin dissolved with fish or corn oil and microemulsified using non-ionic surfactant surfynol 465 W; alternatively LNCaP cells were treated with curcumin directly dispersed in fish or corn oil (0-50 μM) for 24 -72-144 hr. Cell viability was determined using resazurin (Vision blueTM) fluorescence assay. Glutathione status was determined by monochlorobimane (MCB) assay. Results: Treatment with 0-34 μM of microemulsified curcumin produced moderate cytotoxic effect on LNCaP cells, no 50% reduction of cell viability was observed graphically. However, when LNCaP cells were treated with curcumin dispersed with corn oil the concentration or 50% reduction of cell viability (IC50) was 12-45 μM. Similarly for cells treated with curcumin dispersed with fish oil, the IC50 was between 20-40 μM. Cytotoxic doses of curcumin dispersed with corn or fish oil increased GST status in cells by 272-656% (p =<0.01). Conclusion: Microemulsified curcumin formulation prepared using fish or corn oil and surfynol 465 W surfactant had an inhibitory effect on viability of LNCaP cells as did direct dispersion of curcumin in fish or corn oil coupled with the ability for inducing intracellular GST status in LNCaP cells.
    • Antioxidant, Anticancer and Antibacterial Activity of Withania somnifera Aqueous Root Extract

      Barnes, D. A.; Barlow, R.; Nigam, P. S.; Owusu-Apenten, Richard K.; University of Chester, University of Ulster (Sciencedomain international, 2015-11-10)
      Aims: To evaluate total antioxidant capacity, anticancer activity and antibacterial effects Withania somnifera aqueous-root extracts. Study Design: In vitro study. Place of Study: School of Biomedical Sciences, Ulster University, UK. Methodology: Total antioxidant capacity (TAC) of whole powder and freeze dried W. somnifera aqueous-root extracts was determined using FRAP, DPPH, Folin and ABTS assays. Anticancer activity was accessed using MDA-MB-231 breast cells and Sulforhodamine B staining for cell viability. Antibacterial activity was by disk diffusion assay with penicillin, amoxicillin and streptomycin as positive controls. Results: The TAC for W. somnifera extract was 86, 47, 195,or 443 gallic acid equivalents per 100g dry basis (mgGAE/ 100 g) using FRAP, DPPH, Folin or ABTS assays, respectively. Corresponding TAC values for freeze dried W. somnifera aqueous-root extract were, 418, 553, 1898 or, 1770 (mgGAE/100 g). W. somnifera aqueous-root extract inhibited MDA-MB-231 cell proliferation in a dose-dependent manner with IC50 = 0.19 mg/ml (21 µM GAE). Nil antibacterial effects were detected for freeze dried W. somnifera extract (0-1 mg/ml) across six species of bacteria tested. Conclusion: Withania somnifera root water extract showed significant antioxidant and anticancer activity for MDA-MB-231 breast cancer cells but no antibacterial activity under the conditions of this study.
    • Antioxidant, Anticancer and Antimicrobial, Effects of Rubia cordifolia Aqueous Root Extract

      Barlow, R; Barnes, D; Campbell, A; Nigam, PS; Owusu-Apenten, Richard K.; University of Chester, University of Ulster (Sciencedomain international, 2015-11-10)
      Aims: To evaluate the total antioxidant capacity (TAC) of Rubia cordifolia root extracts, to test anticancer activity against MDA-MB-231 breast cancer cell lines, and to evaluate antimicrobial activity of the same extract versus six Gram-positive and negative bacteria. Study Design: In vitro. Place of Study and Duration: School of Biomedical Sciences, Ulster University, July 2014-Sept 2015. Methodology: TAC was tested using ABTS, DPPH, FRAP and Folin assays and values were expressed as mg-gallic acid equivalents per 100 g (GAE/100 g) of sample. Anticancer properties were examined against MDA-MB-231 breast cancer cell lines using Sulforhodamine B assay. Antimicrobial activity was examined using a disk diffusion assay with three Gram-positive (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi) bacteria. Results: TAC of dry extracts of Rubia cordifolia ranged from 523±43 to 4513±208 (mg GAE mg/100 g) depending on the method of analysis, ABTS> FRAP> Folin > DPPH methods. R. cordifolia dry extract showed cytotoxicity against MDA-MB-231 with IC50 = 44 µg/ml or 5.1µM GAE. No antimicrobial activity was observed against the three Gram-positive, or three Gram-negative bacterial species using the water extract or R. cordifolia. Conclusion: R. cordifolia aqueous extract possess high total antioxidant capacity but values depend on the method of analysis. R. cordifolia extract inhibits MDA-MB-231 breast cancer cells proliferation but nil anti-bacterial activity was observed for three Gram-positive and three Gram-negative bacterial strains tested.
    • The low-risk perception of developing type 2 diabetes among women with a previous history of gestational diabetes: a qualitative study

      Sharma, Manisha; Purewal, Tejpal Singh; Fallows, Stephen; Kennedy, Lynne (Wiley, 2019-02-13)
    • Rapid Colorimetric Determination of Methylglyoxal Equivalents for Manuka Honey

      Kwok, T. H.; Kirkpatrick, G; Yusof, H. I. Mohd; Portokalakis, I; Nigam, P.S.; Owusu-Apenten, Richard Kwasi; University of Chester, University of Ulster (Sciencedomain international, 2016-06-14)
      Aims: Realization of a rapid colorimetric assay for monitoring levels of methylglyoxal and other dicarbonyl compounds from Manuka honey. Methods: N-acetyl cysteine (NAC) and 2, 4-dinitrophenylhydrazine (DNPH) were adopted as reagents for methylglyoxal colorimetric analysis of honey at 288 or 525 nm, respectively. Results and Discussion: NAC and DNPH produced linear responses for methylglyoxal with:(i) regression coefficient (R2) equal to 0.99 or 0.97, (ii) molar absorptivity (measure of sensitivity) equal to 287±11 or 14189±498 M-1 cm-1, (iii) a minimum detectable concentration (MDC) of 0.18 mM vs 7.3 µM, (iv) upper linearity limit of linearity (ULL) equal to 4mM or 83 µM, and (v) a day-to-day precision of 16.0 and 18.3%, respectively. Low interferences occurred with reducing sugars, glyoxal or 3-deoxy-D-glucosone. For honey with a unique manuka factor (UMF) rating 5+ to UMF18+, the net concentration of dicarbonyl compounds ranged from 1069 mg-methylglyoxal equivalence per kg (mg MeGEq /kg) to 2208 (mg MeGEq /kg) using the NAC assay. For the DNPH assay, the apparent dicarbonyl concentration was 350 to 1009-mg MeGEq /kg honey. Measures of methylglyoxal equivalences were strongly correlated with the UMF rating for honeys (R2=0.98-0.99). Conclusion: The proposed colorimetric analysis of methylglyoxal equivalence in Manuka honey is feasible proposition. Further work is needed for method validation.
    • From Surveillance to Intervention: Overview and Baseline Findings for the Active City of Liverpool Active Schools and SportsLinx (A-CLASS) Project.

      McWhannell, Nicola; email:; Foweather, Lawrence; orcid: 0000-0001-9851-5421; email:; Graves, Lee E F; orcid: 0000-0002-3323-313X; email:; Henaghan, Jayne L; email:; Ridgers, Nicola D; orcid: 0000-0001-5713-3515; email:; Stratton, Gareth; orcid: 0000-0001-5618-0803; email: (2018-03-23)
      This paper outlines the implementation of a programme of work that started with the development of a population-level children's health, fitness and lifestyle study in 1996 (SportsLinx) leading to selected interventions one of which is described in detail: the Active City of Liverpool, Active Schools and SportsLinx (A-CLASS) Project. The A-CLASS Project aimed to quantify the effectiveness of structured and unstructured physical activity (PA) programmes on children's PA, fitness, body composition, bone health, cardiac and vascular structures, fundamental movement skills, physical self-perception and self-esteem. The study was a four-arm parallel-group school-based cluster randomised controlled trial (clinical trials no. NCT02963805), and compared different exposure groups: a high intensity PA (HIPA) group, a fundamental movement skill (FMS) group, a PA signposting (PASS) group and a control group, in a two-schools-per-condition design. Baseline findings indicate that children's fundamental movement skill competence levels are low-to-moderate, yet these skills are inversely associated with percentage body fat. Outcomes of this project will make an important contribution to the design and implementation of children's PA promotion initiatives.
    • Internal loads, but not external loads and fatigue, are similar in young and middle-aged resistance trained males during high volume squatting exercise.

      Fernandes, John; Lamb, Kevin; Twist, Craig; University of Chester (MDPI Basel, 2018-08-22)
      Little is known about the internal and external loads experienced during resistance exercise, or the subsequent fatigue-related response, across different age groups. This study compared the internal (heart rate, OMNI ratings of perceived exertion (RPE), session RPE) and external loads (peak velocity and power and volume load) during high volume squatting exercise (10 10 at 60% one-repetition maximum (1RM)) and the fatigue-related response (maximal voluntary contraction (MVC), voluntary activation (VA), resting doublet force, peak power, and blood lactate) in young (n = 9; age 22.3 1.7 years) and middle-aged (n = 9; age 39.9 6.2 years) resistance-trained males. All internal load variables and peak velocity illustrated unclear differences between groups during exercise. Peak power and volume load were likely higher in the young group compared to their middle-aged counterparts. The unclear differences in MVC, VA and blood lactate between groups after exercise were accompanied by very likely greater decrements in resting doublet force and peak power at 20 and 80% 1RM in the middle-aged group compared to the young group. These data indicate that internal load is not different between young and middle-aged resistance-trained males, though certain external load measures and the fatigue response are.