• A qualitative evaluation of the Western Cheshire Community Weight Management Programme - Step by Step Healthy Weight and Lifestyle Programme - Interim Report

      Hughes, Deanna; Perry, Catherine; Thurston, Miranda; Centre for Public Health Research, University of Chester (Centre for Public Health Research, University of Chester, 2010-03)
      This report is an interim report for the evaluation of a community weight management programme, Step by Step. Western Cheshire PCT commissioned the University of Chester to evaluate the programme. The overall aim of the evaluation was to reveal the elements of the service that are most likely to increase the effectiveness and efficiency of the programme.
    • Quality in ICT-based higher education: Some introductory questions

      Fallows, Stephen; Bhanot, Rakesh; University College Chester ; Coventry University (RoutledgeFalmer, 2005)
      This book chapter focus on several key themes in quality in ICT-based higher education - training, e-mail, educational opportunities, presentation, and the electronic library.
    • Quantification of physical contact and its influence on simulated performance and recovery in rugby players.

      Twist, Craig; Norris, Jonathan (University of Chester, 2018-08-07)
      The aim of this thesis was to investigate the influence of physical collisions on internal (physiological and perceptual) and external (locomotive and accelerometer) load during simulated rugby league performance and fatigue responses in the days after. Chapter 4 examined the influence of physical contact type on internal and external load using a traditional soft tackle bag and custom-built tackle sled. Using a traditional tackle bag to simulate physical collisions resulted in likely faster sprint to contact speed (16.1 ± 1.5 c.f. 14.8 ± 1.1 km.h -1 ) but possibly lower overall high-speed running distance (27.7 ± 2.4 c.f. 28.4 ± 2.6 m.min-1 ). Also, the heavier tackle sled likely increased time at 91-100% HRpeak (12:58 ± 13:21 c.f. 6:44 ± 8:06 min:s) and resulted in greater lower limb fatigue reflected by the likely larger decrease in countermovement jump (CMJ) performance (5.9 ± 4.9 c.f. 2.6 ± 5.4%). Also of note was the variation in number of tackles detected using the automatic tackle detection feature compared to the actual number in the match simulation. During the Bag and Sled simulations ~53 and ~59 tackles were detected compared to 48 performed. The purpose of Chapter 5 was to investigate the influence of sprint to contact speed and contact type on automatic tackle detection using microtechnology. Repetitions were divided into three speed categories; walking, jogging and striding (1, 2.5 and 4 m.s -1 ) and four conditions: i) no contact standing upright (NCST), ii) no contact dropping to the ground in a prone position (NCGR), iii) contact with the tackle bag and remaining upright (CST), iv) contact with the tackle bag and going to ground (CGR). Similar tackle detection accuracy was observed between NCGR and CST conditions with one tackle observed in 41 and 43% of trials, respectively. While CGR resulted in the greatest frequency of correct tackle detection (62%), during 16% of trials two tackles were detected. During NCST, there were no tackles detected and 100% accuracy. The PlayerLoadTM results demonstrated that the metric can detect differences in movement speed, the inclusion of physical contact and changes in orientation during short periods of activity (8-10 s). In Chapter 6 the rugby league movement simulation protocol for interchange players (RLMSP-i) was modified to include a tackle shield collision to investigate the reliability of PlayerLoadTM metrics to quantify collision load. The coefficient of variation (%CV) for locomotive metrics ranged from 1.3 to 14.4%, with greatest variability observed for high-speed running distance (8.0 and 14.4% for Bouts 1 and 2, respectively). Accelerometer metrics CV% were 4.4 to 10.0%, while internal load markers were 4.8 to 13.7%. All variables presented a CV% less than the calculated moderate change during one or both bouts of the match simulation except from high-speed distance (m.min-1 ), %HRpeak and RPE (AU). The aim of Chapter 7 was to investigate the influence of contact type on external load metrics including PlayerLoadTM derivatives whilst controlling for total running distance. Participants were randomly assigned to one group to complete the match simulation with either a tackle shield (n = 10), tackle bag (n = 7) or no-contact (n = 10). Total PlayerLoadTM, PlayerLoadTM 2D (AU), PlayerLoadTM slow (AU) and PlayerLoadTM slow-ratio (%) were analysed from the accelerometer in addition to high- and low-speed running and sprint speed. Total PlayerLoadTM was likely lower for the Bag group compared to the Run group (498 c.f. 460 AU), with no clear differences between the other groups. 3 PlayerLoadTM slow for the Shield group (167 ± 26 AU) was very likely greater than both the Bag (133 ± 11 AU) and Run groups (128 ± 20 AU) but no clear difference was observed between the Bag and Run groups. No differences were observed in PlayerLoadTM 2D between any groups. High-speed running distance was likely lower in the Shield group (1056 ± 225 m) compared to the Bag group (1326 ± 245 m) and very likely lower compared to the Run group (1318 ± 175 m). Total PlayerLoadTM is not sensitive to contact type during simulated rugby league activity but does reflect greater high-speed running distance during a rugby league match simulation. However, PlayerLoadTM slow can detect the types of contact and might be preferred for quantifying match and training loads associated with physical contact. The purpose of the final empirical chapter (Chapter 8) was to determine the influence of contact type on in neuromuscular, perceptual and biochemical parameters associated with exercise-induced muscle damage. The participants were again assigned to one of three groups to complete the match simulation with a tackle shield (n = 6), tackle bag (n = 7) or no contact (n = 7). In addition to internal and external load measured during the match simulation, venous blood, muscle function and soreness measures were collected immediately (+0), +24 and +72 hours after the match simulation. Upper body neuromuscular performance and knee flexion torque likely decreased in the Shield group +0 and +72 hours after the simulation compared to the other groups while CMJ power likely decreased more in the Run group. All three groups demonstrated a very likely increase in IL-6 and IL-10 concentration immediately after the match simulation, but differences between the groups were unclear and values returned to baseline +24 hours after the simulation. In conclusion, current automatic tackle detection metric should be used with caution, particularly in training sessions where physical contact is replicated. Instead PlayerLoadTM and associated derivatives from the embedded accelerometer can provide a useful measure of contact-specific load during training and competitive matches. Physical contact type affected external load by modifying a participant’s running strategy during simulated match performance, thereby influencing site-specific fatigue during and after a simulated rugby league match. However, regardless of contact type, large increases in cytokine and leukocyte concentration are apparent with a return to basal values 24 hours after. Therefore it is not recommended to use such biomarkers in applied settings to quantify the magnitude of muscle damage specifically associated with physical contact.
    • Quantification of the physical and physiological load of a boxing-specific simulation protocol

      Thomson, Edward; Lamb, Kevin L.; University of Chester (Taylor & Francis, 2017-03-24)
      The aim of the study was to determine the physical and physiological responses to simulated amateur boxing of 3 × 3-min rounds. Using an externally valid technical and ambulatory demand, 28 amateur boxers (mean ± SD; age 22.4 ± 3.5 years, body mass 67.7 ± 10.1 kg, stature 171 ± 9 cm) completed the protocol following familiarisation. The physiological load was determined continuously via collection of mean (HRmean) and peak (HRpeak) heart rate, breath-by-breath oxygen uptake ( ̇V O2), aerobic energy expenditure (EEaer), excess carbon dioxide production (CO2excess), ratings of perceived exertion (RPE) and post-performance blood lactate. Physical performance was quantified as the acceleration delivered to the target by punches. HRmean and HRpeak were found to exceed 165 and 178 b min−1, absolute ̇V O2 > 124.6 ml kg−1, EEaer > 30.7 kcal min−1 and acceleration via 78 punches >2697 g during each round. Mean blood lactate (4.6 mmol l−1) and CO2excess (438.7 ml min−1) were higher than typical resting values reflecting a notable anaerobic contribution. RPEs reinforced the intensity of exercise was strenuous (>6–8). For all measures, there were typical increases (p < 0.05; moderate ES) across rounds. Accordingly, boxers might consider high-intensity (>90% ̇V O2max) interval training in anticipation such exercise yields improvements in aerobic conditioning. Moreover, the current simulation protocol – the boxing conditioning and fitness test – could be used as a form of training per se and as a means to monitor intervention-based changes in aspects of boxing-related physiology and performance. 1.
    • A quantified ethogram for oviposition in triturus newts: Description and comparison of T. helveticus and T. vulgaris

      Norris, Karen M.; Hosie, Charlotte A.; University of Chester (Ethologische Gesellschaft e.V., 2005)
      Female newts of the genus Triturus deposit and wrap their eggs individually in the submerged leaves of aquatic macrophytes. Although this behaviour has previously been described, the different elements of the oviposition process have not been fully characterized nor any attempt made to quantify the behavioural elements. The study examined the oviposition behaviour of the two similarly sized species, Triturus helveticus and T. vulgaris on a standardized substrate macrophyte, Rorippa nasturtium–aquaticum. Continuous focal sampling was used to develop a baseline of discrete behavioural elements enabling quantification and comparison of oviposition behaviour between the two species. The results showed that the same pattern of elements was followed for each egg laid and the same key elements of the process were present in each newt species. Although these are broadly similar in size, there were striking differences in certain aspects of the oviposition sequence between the two species. Key findings were that leaf sniffing and leaf flexing and a measure of the duration of ovipositing were all significantly greater in females of T. helveticus and females of T. vulgaris laid significantly more eggs than those of T. helveticus in a standard observation period. The work presented here defines a baseline ethogram and shows how it can be used to reveal quantifiable differences in closely related species. This demonstrates its value in furthering our understanding of oviposition – a key aspect of female behaviour currently understudied in Triturus behavioural ecology, despite its intrinsic interest and value in understanding recruitment and maintenance of populations.
    • Quantifying measurement error

      Lewis, Stephen J.; Chester College of Higher Education (Oxbow Books (for the Osteoarchaeological Research Group), 1999)
      It is important for workers to have some estimate of the degree of error evident when measuring objects. Although many use their own "rule-of-thumb" to give them the personal satisfaction that they are working accurately, measures of error, or conversely reliability, are rarely given in the lierature. Some simple, useful equations are given that may be used privately or when reporting metrical work.
    • Questioning the role of Darwinian medicine

      Lewis, Stephen J.; Chester College of Higher Education (2001-09)
      Darwinian medicine as a distinct scientific discipline can be traced to George Williams' and Randolph Nesse's paper 'The Dawn of Darwinian Medicine' (Q Rev. Biol. 66: 1-22, 1991). Ten years on, at what is still the dawn of a new millennium, it is timely to review the current state of Darwinian medicine and to assess some of its still latent potentialities. Nesse remains the main protagonist of a Darwinian approach to medicine. Important work by others has appeared but his and Williams' 'Evolution and Healing' (Weidenfeld and Nicolson, 1995)†, although written for the popular press, remains the primary text of the whole discipline. Distinct lines of academic interest and inquiry are, however, emerging within Darwinian medicine and it has found inclusion in a number of undergraduate curricula. Sufficient interest exists to suggest that it will survive as a discipline. But as a discipline, Darwinian medicine needs more than a single protagonist – it needs serious critical attention. It is important, therefore, to ask in what form the discipline is to persist and into what new areas it might go. Fundamental to such questions is the relationship between Darwinian medicine (as a scientific discipline) and Western clinical medicine (as a profession). It is suggested that, rather than try to gain direct acceptance by the medical profession, Darwinian medicine might seek to establish itself more firmly within the academic disciplines of evolutionary and human biology. It is suggested that it give special attention to identifying and making its own distinctive voice heard as a biological science of health and disease distinct from medicine – a voice that clinical medicine, drawing as ever from allied sciences, cannot then afford to ignore. To this end, the question of what might be the “zeroth law” of Darwinian medicine is posited.
    • A questionnaire survey of the provision of training in human sexuality in schools of nursing in the UK

      Astbury-Ward, Edna; Glyndŵr University (Taylor & Francis, 2011-10-05)
      The inconsistent and haphazard approach to the provision of training in human sexuality to health professionals in the UK and elsewhere has been a matter of concern for over half a century. This article discusses the implications of findings from a questionnaire survey of 41 schools of nursing in the UK regarding their provision of training in human sexuality. Schools of nursing were chosen, as nurses form by far the largest employment group in the NHS today. The aim of this questionnaire survey was to obtain information on the provision of training in human sexuality in schools of nursing in the UK. The 20-item mixed qualitative and quantitative questionnaire was designed to elicit maximum information about research questions, it was independently validated after focus group discussion. The results drawn from this study will be primarily presented as observations, rather than statistically tested statements. The provision of training in human sexuality in the pre-registration nursing curricula in the UK is inadequate to meet the current varied needs of patients. On average, a mere 6.8 hours out of a potential 2300 hours of theoretical learning is dedicated to teaching human sexuality in schools of health in the UK. This is considerably less time than is dedicated to other areas of learning within the curriculum. The overall provision of training in human sexuality in the nursing curricula does not seem to have significantly improved, regardless of pleas from such esteemed bodies as the World Health Organisation and others.
    • Rachel Carson

      Burek, Cynthia V.; University of Chester (The National Federation of Women's Institute, 2011)
      This article discusses the life and career of the environmentalist Rachel Carson (1907-1964).
    • RAISIN, Catherine Alice (1855-1945)

      Burek, Cynthia V.; University College Chester (Thoemmes Continuum, 2004)
      This dictionary entry discusses This article discusses the life and career of British geologist Catherine Raisin (1855-1945) including her impact on the education system.
    • Raising awareness of anaerobic digestion in the UK - Views of key stakeholders

      Duruiheoma, Franklin I.; Burek, Cynthia V.; Bonwick, Graham A.; Alexander, Roy; University of Chester (Macrothink Institute, 2014-12-31)
      Meeting rising energy demand and sustainable development goals at the same time is a major challenge for policy makers in the 21st century. The situation is further stressed by a rising world population, climate change, natural disasters and food security concerns. Renewable energy technologies such as anaerobic digestion (AD) proffer one solution for policy makers to overcome some of the challenges to sustainable development. The technology has been widely adopted in some parts of Europe (e.g. Germany, Denmark, Austria and Sweden), the United States and also parts of Asia and Africa. However in the United Kingdom (UK), the technology is under-developed, as was recognised in its anaerobic digestion strategy and action plan of 2011. This study focused on identifying options for raising awareness of AD technology in the UK. 21 key stakeholders divided into groups according to their expertise, were interviewed to explore their views on the areas of focus in the UK strategy and action plan regarding raising awareness of the technology. The results revealed that aligning AD with sustainable development goals, community AD and localism, small AD plants, provision of an available market for AD products, building UK skills and diversifying biogas use from AD are positive options for raising awareness of AD in the UK. Challenges to these options and possible solutions to the challenges were also identified and discussed.
    • RANK, RANKL and osteoprotegerin in bone biology and disease

      Wright, H. L.; McCarthy, Helen S.; Middleton, Jim F.; Marshall, Michael J.; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry (2009)
      This review discusses the latest news and views on the mechanisms controlling bone resorption in normal and pathological conditions.
    • Rapid Colorimetric Determination of Methylglyoxal Equivalents for Manuka Honey

      Kwok, T. H.; Kirkpatrick, G; Yusof, H. I. Mohd; Portokalakis, I; Nigam, P.S.; Owusu-Apenten, Richard Kwasi; University of Chester, University of Ulster (Sciencedomain international, 2016-06-14)
      Aims: Realization of a rapid colorimetric assay for monitoring levels of methylglyoxal and other dicarbonyl compounds from Manuka honey. Methods: N-acetyl cysteine (NAC) and 2, 4-dinitrophenylhydrazine (DNPH) were adopted as reagents for methylglyoxal colorimetric analysis of honey at 288 or 525 nm, respectively. Results and Discussion: NAC and DNPH produced linear responses for methylglyoxal with:(i) regression coefficient (R2) equal to 0.99 or 0.97, (ii) molar absorptivity (measure of sensitivity) equal to 287±11 or 14189±498 M-1 cm-1, (iii) a minimum detectable concentration (MDC) of 0.18 mM vs 7.3 µM, (iv) upper linearity limit of linearity (ULL) equal to 4mM or 83 µM, and (v) a day-to-day precision of 16.0 and 18.3%, respectively. Low interferences occurred with reducing sugars, glyoxal or 3-deoxy-D-glucosone. For honey with a unique manuka factor (UMF) rating 5+ to UMF18+, the net concentration of dicarbonyl compounds ranged from 1069 mg-methylglyoxal equivalence per kg (mg MeGEq /kg) to 2208 (mg MeGEq /kg) using the NAC assay. For the DNPH assay, the apparent dicarbonyl concentration was 350 to 1009-mg MeGEq /kg honey. Measures of methylglyoxal equivalences were strongly correlated with the UMF rating for honeys (R2=0.98-0.99). Conclusion: The proposed colorimetric analysis of methylglyoxal equivalence in Manuka honey is feasible proposition. Further work is needed for method validation.
    • A re-appraisal of the reliability of the 20 m multi-stage shuttle run test

      Lamb, Kevin L.; Rogers, Louise; University of Chester (Springer-Verlag, 2007-03-07)
    • "Read with caution": A reply to Pickett et al

      Smith, Dave; Wright, Caroline J.; Bruce-Low, Stewart; Hale, Bruce; University College Chester ; University College Chester ; University College Chester ; Penn State University (British Association of Sport and Medicine, 2005)
      This article critiques a study by Pickett Lewis & Cash titled "Men, muscles, and body image: comparisons of competitive bodybuilders, weigh trainers, and athletically active controls" published in the British Journal of Sports Medicine in 2005.
    • Recapture rates and habitat associations of White-faced Darter Leucorhinnia dubia on Fenn's and Whixall Moss, Shropshire, UK

      Davies, Rachel; von Hardenberg, Achaz; Geary, Matthew; Conservation Biology Research Group, Department of Biological Sciences, University of Chester, CH1 4BJ (British Dragonfly Society, 2018-10-01)
      Land-use change and habitat loss are important drivers of biodiversity decline at both global and local scales. To protect species from the impacts of land-use change it is important to understand the population dynamics and habitat associations across these scales. Here we present an investigation into the survival and habitat preferences of Leucorrhinia dubia at the local scale at Fenn’s and Whixall Moss, Shropshire, UK. We used mark-release-recapture methods to investigate survival and used sightings of individual dragonflies along with habitat data to investigate habitat preference. We found that survival between capture-visits was very low and that L. dubia showed a clear preference for the open moss habitat on this site. In both cases, we found that the detectability, either through sightings or recaptures, was potentially very low and suggest that this should be taken into account in future analyses. We suggest that by encouraging recorders to submit complete lists and to repeat visits to sites detectability could be easily estimated for dragonfly species and incorporating this into analyses would improve estimates fo population trends and habitat associations.
    • Reciprocal regulation of GAS5 lncRNA levels and mTOR inhibitor action in prostate cancer cells.

      Yacqub-Usman, Kiren; Pickard, Mark R.; Williams, Gwyn T.; Keele University (Wiley, 2015-02-03)
      BACKGROUND: New therapies are required for castrate-resistant prostate cancer (CRPC), and growth-arrest specific 5 (GAS5) lncRNA, which riborepresses androgen receptor action, may offer novel opportunities in this regard. This lncRNA promotes the apoptosis of prostate cancer cells and its levels decline as prostate cancer cells acquire castrate-resistance, so that enhancing GAS5 expression may improve the effectiveness of chemotherapies. Since GAS5 is a member of the 5' terminal oligopyrimidine gene family, we have examined mTOR inhibition as a strategy to increase GAS5 expression. Furthermore, we have determined if GAS5 itself mediates the action of mTOR inhibitors, as demonstrated for other chemotherapeutic agents in prostate cancer cells. METHODS: The effects of mTOR inhibitors on GAS5 lncRNA levels and cell growth were determined in a range of prostate cancer cell lines. Transfection of cells with GAS5 siRNAs and plasmid constructs was performed to determine the involvement of GAS5 lncRNA in mTOR inhibitor action. RESULTS: First generation mTORC1, combined mTORC1/mTORC2 and dual PI3K/mTOR inhibitors all increased cellular GAS5 levels and inhibited culture growth in androgen-dependent (LNCaP) and androgen-sensitive (22Rv1) cell lines, but not in androgen-independent (PC-3 and DU 145) cell lines. The latter exhibited low endogenous GAS5 expression, and GAS5 silencing in LNCaP and 22Rv1 cells decreased the sensitivity to mTOR inhibitors, whereas transfection of GAS5 lncRNA sensitized PC-3 and DU 145 cells to these agents. CONCLUSION: mTOR inhibition enhances GAS5 transcript levels in certain prostate cancer cell lines. This selectivity is likely to be related to endogenous GAS5 expression levels, since GAS5 lncRNA is itself required for mTOR inhibitor action in prostate cancer cells.
    • Recovery of high mountain Alpine lakes after the eradication of introduced brook trout Salvelinus fontinalis using non-chemical methods

      Tiberti, Rocco; Bogliani, Giuseppe; Brighenti, Stefano; Iacobuzio, Rocco; Liautaud, Kevin; Rolla, Matteo; von Hardenberg, Achaz; Bassano, Bruno; University of Pavia; Gran Paradiso National Park; University of Trento; Fondazione Edmund Mach; Swansea University; University of Chester (Springer, 2018-10-31)
      Fish stocking is a serious threat to originally fishless mountain lakes. We used non-chemical eradication methods (i.e. gillnetting and electrofishing) in four high mountain lakes in the Gran Paradiso National Park (Western Italian Alps) to eradicate alien brook trout Salvelinus fontinalis. Data of amphibians, macroinvertebrates, zooplankton, chlorophyll-a, nutrient concentrations, and water transparency were used as indicators of the recovery process. All treated lakes were returned to their original fishless condition in spite of their different sizes and habitat complexity, without permanent negative side-effects for native species. Several ecological indicators showed that many impacts of introduced fish can be reversed over a short time period following eradication. The present study adds to a still growing body of specialized literature on the recovery of habitats after the eradication of alien species and provides further evidence that physical eradication methods are effective and can be part of a more general strategy for the conservation of high mountain lake biota.
    • Rediscovering and conserving the Lower Palaeozoic 'treasures' of Ethel Woods (nee Skeat) and Margaret Crosfield in northeast Wales

      Burek, Cynthia V.; Malpas, Jacqui A.; University of Chester ; NEWRIGS, Millenium EcoCentre in Wrexham (2007)
      This book chapter explores, within a historical context, the importance of geoconservation of not only sitesbut also artefacts, collections and specimens as well as letters and original documents. It sets but the search and finding of sites in northeast Wales and materials thought lost then found and the subsequent nomination of Regionally Important Geological/Geomorphological Sites (RIGS) conservation status of the sites to safeguard them for the future. It is important to note that RIGS can be designated for their historical value alone, which is in contrast to Sites of Special Scientific Inleresi (SSSIs), which are protected solely for their national scientific and research value. The role of Ethel Woods (nee Skeat) and Margaret Crosfield in developing an understanding of the geological history of northeast Wales had been lost over time. This paper contains biographical sketches of the two women, followed by their Lower Palaeozoic lithological, structural and grap-tolite research and places it in an historical context. This case study illustrates how female curiosity, perseverance and attention to detail unearthed previously forgotten treasures. The importance of conserving their sites, specimens and sketch field notebooks in our electronic and throw-away age is vital. The role of the North East Wales Regionally Important Geological/ Geomorphological Sites (NEWRIGS) in conserving this information is put forward as an example of good practice.
    • Reduction in high intensity activity during senior elite rugby league matches

      Sykes, Dave; Twist, Craig; Lamb, Kevin L.; Nicholas, Ceri; University of Chester (2009-06)