• Non-territorial GPS-tagged golden eagles Aquila chrysaetos at two Scottish wind farms: avoidance influenced by preferred habitat distribution, wind speed and blade motion status

      Fielding, Alan H; Anderson, David; Benn, Stuart; Dennis, Roy; Weston, Ewan; Whitfield, Philip; Natural Research Ltd; Forestry and Land Scotland; RSPB Scotland; Roy Dennis Wildlife Foundation; University of Chester
      Wind farms can have two broad potential adverse effects on birds via antagonistic processes: displacement from the vicinity of turbines (avoidance), or death through collision with rotating turbine blades. These effects may not be mutually exclusive. Using detailed data from 99 turbines at two wind farms in central Scotland and thousands of GPS-telemetry data from dispersing golden eagles, we tested three hypotheses. Before-and-after-operation analyses supported the hypothesis of avoidance: displacement was reduced at turbine locations in more preferred habitat and with more preferred habitat nearby. After-operation analyses (i.e. from the period when turbines were operational) showed that at higher wind speeds and in highly preferred habitat eagles were less wary of turbines with motionless blades: rejecting our second hypothesis. Our third hypothesis was supported, since at higher wind speeds eagles flew closer to operational turbines; especially – once more – turbines in more preferred habitat. After operation, eagles effectively abandoned inner turbine locations, and flight line records close to rotor blades were rare. While our study indicated that whole-wind farm functional habitat loss through avoidance was the substantial adverse impact, we make recommendations on future wind farm design to minimise collision risk further. These largely entail developers avoiding outer turbine locations which are in and surrounded by swathes of preferred habitat. Our study illustrates the insights which detailed case studies of large raptors at wind farms can bring and emphasises that the balance between avoidance and collision can have several influences.
    • Responses of dispersing GPS-tagged Golden Eagles (Aquila chrysaetos) to multiple wind farms across Scotland

      Fielding, Alan H; Anderson, David; Benn, Stuart; Dennis, Roy; Geary, Matthew; Weston, Ewan; Whitfield, Phil; Natural Research Ltd; Forestry and Land Scotland; RSPB Scotland; Roy Dennis Wildlife Foundation; University of Chester (Wiley, 2021-07-20)
      Wind farms may have two broad potential adverse effects on birds via antagonistic processes: displacement from the vicinity of turbines (avoidance), or death through collision with rotating turbine blades. Large raptors are often shown or presumed to be vulnerable to collision and are demographically sensitive to additional mortality, as exemplified by several studies of the Golden Eagle Aquila chrysaetos. Previous findings from Scottish Eagles, however, have suggested avoidance as the primary response. Our study used data from 59 GPS-tagged Golden Eagles with 28 284 records during natal dispersal before and after turbine operation < 1 km of 569 turbines at 80 wind farms across Scotland. We tested three hypotheses using measurements of tag records’ distance from the hub of turbine locations: (1) avoidance should be evident; (2) older birds should show less avoidance (i.e. habituate to turbines); and (3) rotor diameter should have no influence (smaller diameters are correlated with a turbine’s age, in examining possible habituation). Four generalized linear mixed models (GLMMs) were constructed with intrinsic habitat preference of a turbine location using Golden Eagle Topography (GET) model, turbine operation status (before/after), bird age and rotor diameter as fixed factors. The best GLMM was subsequently verified by k-fold cross-validation and involved only GET habitat preference and presence of an operational turbine. Eagles were eight times less likely to be within a rotor diameter’s distance of a hub location after turbine operation, and modelled displacement distance was 70 m. Our first hypothesis expecting avoidance was supported. Eagles were closer to turbine locations in preferred habitat but at greater distances after turbine operation. Results on bird age (no influence to 5+ years) rejected hypothesis 2, implying no habituation. Support for hypothesis 3 (no influence of rotor diameter) also tentatively inferred no habituation, but data indicated birds went slightly closer to longer rotor blades although not to the turbine tower. We proffer that understanding why avoidance or collision in large raptors may occur can be conceptually envisaged via variation in fear of humans as the ‘super predator’ with turbines as cues to this life-threatening agent.
    • Evaluation of the Feasibility, Reliability, and Repeatability of Welfare Indicators in Free-Roaming Horses: A Pilot Study.

      Harley, Jessica; Stack, J.D.; Braid, H; McLennan, Krista M.; Stanley, Christina; University of Chester; University of Liverpool
      Validated assessment protocols have been developed to quantify welfare states for intensively managed sport, pleasure, and working horses. There are few protocols for extensively managed or free-roaming populations. Here, we trialed welfare indicators to ascertain their feasibility, reliability, and repeatability using free-roaming Carneddau Mountain ponies as an example population. The project involved (1) the identification of animal and resource-based measures of welfare from both the literature and discussion with an expert group; (2) testing the feasibility and repeatability of a modified body condition score and mobility score on 34 free-roaming and conservation grazing Carneddau Mountain ponies; and (3) testing a prototype welfare assessment template comprising 12 animal-based and 6 resource-based welfare indicators, with a total of 20 questions, on 35 free-roaming Carneddau Mountain ponies to quantify inter-assessor reliability and repeatability. This pilot study revealed that many of the indicators were successfully repeatable and had good levels of inter-assessor reliability. Some of the indicators could not be verified for reliability due to low/absent occurrence. The results indicated that many animal and resource-based indicators commonly used in intensively managed equine settings could be measured in-range with minor modifications. This study is an initial step toward validating a much-needed tool for the welfare assessment of free-roaming and conservation grazing ponies.
    • Public Policy for Obesity Prevention in Lebanon

      Fallows, Stephen; Kennedy, Lynne; Al Kattan, Malika (University of Chester, 2020-12)
      Background: In Lebanon, the population is experiencing nutrition transition and a high prevalence of obesity. Studies have shown that the Lebanese people are shifting their dietary behaviours from the traditional Lebanese diet, a variation of the Mediterranean diet, towards a westernised diet and are being physically inactive. To achieve a population behaviour change and address the obesity epidemic, the root causes of these behaviours and food choices should be explored and addressed. This approach requires joint efforts from different sectors such as the government, healthcare, and civil society to develop action plans and enact policies. In Lebanon, the factors that influence eating behaviours and physical activity are still not explored, national level policies are still lacking, and researchers have called for an urgent need to implement policies to halt the rise of obesity prevalence. Aims and objectives: The general aim of this thesis is to explore the perceived factors that influence adults’ eating behaviours and physical activity using the socio-ecological model as the conceptual framework. In addition, this PhD research explores the relevant contribution and the position of different stakeholders towards obesity prevention in Lebanon. Finally, this thesis draws on lessons learned from countries worldwide that help in developing guidelines for obesity prevention in Lebanon. Methods: Two studies were conducted for this PhD research. In the first study, the Photovoice method was used to engage Lebanese adults from the North Governorate to explore different factors that influence their eating behaviours and physical activity and to identify their perceptions towards the actions needed to address obesity. The second study involved a series of one-to-one face-to-face semi-structured interviews which were conducted with key stakeholders from different public and private sectors (government, civil society, education, and healthcare). These interviews aimed to explore the key stakeholders’ perceptions towards obesity and its causes, their relevant contribution towards obesity prevention, their priority of action to address obesity, and finally the barriers to effective action to reduce obesity in Lebanon. Results: The findings of the two qualitative studies show that obesity is commonly perceived as a body image issue rather than a health issue. Some of the main factors that were perceived to influence Lebanese adults’ eating behaviours and physical activity were: females’ (wives’ and mothers’) employment, social gatherings, and social norms (e.g. food and meal sharing), safety concerns, the availability of countless unhealthy food options that are relatively cheaper than healthy food, the lack of a physical environment that encourages physical activity, and food marketing and advertisements. The results of the stakeholder’s interviews show that the key stakeholders are aware of the nutrition transition and some of the underlying environmental factors that caused these behaviour changes. Yet, most of the key stakeholders framed obesity in an “individualistic approach” by focusing on unhealthy dietary behaviours, the physical inactivity and placed the responsibility on individuals for their lifestyle. Due to their approach, most of them focused their actions on raising awareness such as the role of education. Some of the barriers to effective policy action to address obesity in Lebanon are the political instability, lack of public demand for action, and lack of coordinated multi-sectoral actions. Implications: The recognition of obesity as a disease as well as strengthening the public, stakeholders’, and policymakers’ support towards obesity prevention strategies are essential to overcome the “policy inertia” in Lebanon. The key areas that need to be addressed for obesity prevention are raising awareness on obesity health consequences, increasing access to healthy food in public and private settings, limiting unhealthy food advertisements, and fiscal measures such as taxing unhealthy food and subsidising healthy food. To facilitate the implementation of policies that aim to prevent obesity, a systematic plan of action should be developed. To engage stakeholders from different sectors and establish a national multi-sectoral collaboration, national strategies and policies that follow a Health in all Policies Approach (HiPA) are recommended. This requires strong leadership from the government. Conclusions: There is an urgent need to shift the actions beyond targeting the individual by implementing public policies to address the obesogenic environment in Lebanon. Recommendations for research, policy actions, and practice are provided to guide the action towards establishing an appropriate public policy that recognises the need to adopt a socioecological model of implementation.
    • Attitudes of Female Warders towards inmate who self-harm: A pilot Exploratory Study from an Inner-city Prison in South India

      Jones, Steven; Kumar, Pradeep; Eost-Telling, Charlotte; Kirshna, Murali; University of Chester (The Indian Society of Criminology; National Law University Delhi, 07-2020)
      Self-harm is a global public health challenge. The management and treatment of those who self-harm is emotionally challenging, and can sometimes manifest in negative attitudes amongst staff who provide care. Health professional’s attitudes towards deliberate self-harm have been studied globally, however, evidence regarding prison staff attitudes is sparse, and particularly lacking in India. The primary aim of this study was to explore the attitudes of female prison warders towards prison inmates who self-harm in an Indian setting. A cross-sectional survey using a questionnaire to measure knowledge and attitudes was administered to prison warders from one city prison in South India. Out of the 210 approached to participate, 170 female warders completed the survey questionnaire. In general, sociodemographic factors of the prison warders were unrelated to their attitudes towards self-harm, and a negligible few had received any training specific to self-harm. A series of educational and skills recommendations have been developed from the study, which can be used to inform intervention initiatives and further, provide a basis for cross-cultural professional comparison studies. Current resources, cultures, practice and context must be considered in any future interventions aimed at progressing the evidence base further. In addition, training and education for staff should include information on knowledge and attitudes about causes, reasons, motivations, forms and purpose of self-harm. Records of staff responses to those who self-harm, irrespective of setting, should include assessment, management, interventions undertaken and incorporated daily practice. Importantly, this work may influence prisoner treatment outcomes and is worthy of further study.
    • Physiological characteristics of female soccer players and health and performance considerations: A narrative review

      Randell, Rebecca; Clifford, Thomas; Drust, Barry; Moss, Samantha; Unnithan, Viswanath; De Ste Croix, Mark; Datson, Naomi; Martin, Daniel; Mayho, Hannah; Carter, James; et al.
      Female soccer has seen a substantial rise in participation, as well as increased financial support from governing bodies over the last decade. Thus, there is an onus on researchers and medical departments to develop a better understanding of the physical characteristics and demands, and the health and performance needs of female soccer players. In this review we discuss the current research, as well as the knowledge gaps, of six major topics: physical demands, talent identification, body composition, injury risk and prevention, health, and nutrition. Data on female talent identification are scarce, and future studies need to elucidate the influence of relative age and maturation selection across age groups. Regarding the physical demands, more research is needed on the pattern of high-intensity sprinting during matches and the contribution of soccer-specific movements. Injuries are not uncommon in female soccer players, but targeting intrinsically modifiable factors with injury prevention programmes can reduce injury rates. The anthropometric and physical characteristics of female players are heterogenous and setting specific targets should be discouraged in youth and sub-elite players. Menstrual cycle phase may influence performance and injury risk; however, there are few studies in soccer players. Nutrition plays a critical role for health and performance and ensuring adequate energy intake remains a priority. Despite recent progress, there is considerably less research in female than male soccer players. Many gaps in our understanding of how best to develop and manage the health and performance of female soccer players remain.
    • Physical health impairment, disability and suicidal intent among self-harm survivors in South India

      Jones, Steven; Somashekar, R; Bharath, D.U; Maiji, Sumanth M; Taylor, Lou; Nagaraj, Santhosh; Krishna, Murali; Mysore Medical College and Research Institute; University of Chester; CSI Holdsworth Memorial Hospital; FRAMe; Viveka Hospital
      Background: Suicide is major public health concern in India. There are limited data examining the relationship between health impairment, disability and severity of suicidal intent. The aim of the study was to examine the associations of health impairment and disability with severity of suicidal intent among survivors following an act of self-harm. Methods: A pilot exploratory study of 453 self-harm survivors from a specialist hospital in South India. Sociodemographics, physical health impairment, disability (WHO Disability Schedule-II), suicidal intent, (Pierce suicide intent scale) and mental disorders were studied. Results: Arthritis was the most common physical impairment among self-harm survivors followed by gastrointestinal, sensory impairment and difficulty with mobilization. Nearly 10% of participants had some degree of functional impairment, with 38% experiencing severe physical pain in the week prior to self-harm. Past history of depression treatment, age, education and occupation influenced positively PSIS scores. There were significant associations between suicidal intent and disability. Conclusions: Indian self-harm survivors indicated complex relationships between physical health, disability and suicidal intent. Understanding these associations may help to develop suicide prevention strategies. Our findings suggest a need for integrating a comprehensive of physical health assessment in self harm survivors.
    • Assessing the behaviour, welfare and husbandry of mouse deer (Tragulus spp.) in European zoos

      Lemos de Figueiredo, Ricardo; Hartley, Matthew; Fletcher, Alison W; University of Chester; Yorkshire Wildlife Park
      Mouse deer are primitive, forest ungulates found in Asia and Africa. Both the lesser mouse deer (Tragulus javanicus) and the Philippine mouse deer (T. nigricans) are managed in European zoos, but inconsistent breeding success between institutions, high neonatal mortality and a general lack of research on their husbandry and behaviour were identified by the coordinators of the European Endangered Species Programme (EEP) and the European Studbook (ESB) for each species, respectively. This study is the first to provide a behavioural description for the Philippine mouse deer and to compile a detailed behavioural repertoire for both species. Our aim was to identify the effects of current husbandry and management practices on the reproduction, behaviour and welfare of zoo-housed mouse deer. Questionnaires on husbandry and management practices were sent to all institutions in the EEP and ESB for the lesser and Philippine mouse deer, respectively, and behavioural data were collected in 15 of these zoos. For the lesser mouse deer, results show a positive effect of vegetation cover on breeding success, foraging and moving behaviours. The provision of enrichment and presence of water ponds also positively affected these behaviours. The time that pairs spent in close proximity had a negative effect on breeding success, but animals in more vegetated enclosures spent less time in close proximity to each other. Results could be partially explained by the natural habitat of this usually solitary species being tropical forest, which provides local water sources and undergrowth for cover from predators. For the Philippine mouse deer there were differences in activity measures recorded between zoos, but the sample size was small with differences in training, enrichment and vegetation cover likely to have been important. In conclusion, since mouse deer inhabit overlapping male and female territories, the usual practice of housing breeding pairs together may be appropriate, but we suggest that they should be provided with opportunities to avoid each other in complex enclosures with ample vegetation cover to maximise their natural behavioural repertoire and breeding success.
    • Street-level green spaces support a key urban population of the threatened Hispaniolan Parakeet Psittacara chloropterus

      Geary, Matthew; Celia, Brailsford; Laura, Hough; Fraser, Baker; Simon, Guerrero; Yolanda, Leon; Nigel, Collar; Stuart, Marsden; University of Chester; Manchester Metropolitan University; Autonomous University of Santo Domingo; Instituto Tecnológico de Santo Domingo; BirdLife International
      While urbanisation remains a major threat to biodiversity, urban areas can sometimes play an important role in protecting threatened species, especially exploited taxa such as parrots. The Hispaniolan Parakeet Psittacara chloropterus has been extirpated across much of Hispaniola, including from most protected areas, yet Santo Domingo (capital city of the Dominican Republic) has recently been found to support the island’s densest remaining population. In 2019, we used repeated transects and point-counts across 60 1 km2 squares of Santo Domingo to examine the distribution of parakeets, identify factors that might drive local presence and abundance, and investigate breeding ecology. Occupancy models indicate that parakeet presence was positively related to tree species richness across the city. N-Mixture models show parakeet encounter rates were correlated positively with species richness of trees and number of discrete ‘green’ patches (> 100 m2) within the survey squares. Hispaniolan Woodpecker Melanerpes striatus, the main tree-cavity-producing species on Hispaniola, occurs throughout the city, but few parakeet nests are known to involve the secondary use of its or other cavities in trees/palms. Most parakeet breeding (perhaps 50‒100 pairs) appears to occur at two colonies in old buildings, and possibly only a small proportion of the city’s 1,500+ parakeets that occupy a single roost in street trees breed in any year. Our models emphasise the importance of parks and gardens in providing feeding resources for this IUCN Vulnerable species. Hispaniola’s urban centres may be strongholds for populations of parakeets and may even represent sources for birds to recolonise formerly occupied areas on the island.
    • Differences in the vertical and horizontal force-velocity profile between academy and senior professional rugby league players, and the implications for strength and speed training.

      Dobbin, N; Cushman, S; Clarke, J; Batsford, J; Twist, C; Manchester Metropolitan University; Reasheath College; England RFU; Salford Red Devils Rugby League Football Club; University of Chester
      BACKGROUND: This study compared the vertical and horizontal force-velocity (FV) profile of academy and senior rugby league players. METHODS: Nineteen senior and twenty academy players from one professional club participated in this study. The vertical FV profile was determined using a series of loaded squat jumps (0.4 to 80 kg) with jump height recorded. The horizontal FV profile involved a 30-m over-ground sprint with split times recorded at 5, 10, 15, 20 and 30 m. Theoretical maximal force (F0), velocity (V0) and power (Pmax), optimal F0 and V0, and activity specific variables (e.g. vertical FV imbalance) were determined. RESULTS: Absolute F0 and Pmax from the vertical and horizontal profile were moderately different between groups (standardised mean difference (SMD) = 0.64-1.20, P <0.001-0.026), whilst for V0, differences were small (SMD = 0.33-0.41, P = 0.149-0.283). Differences in relative F0, Pmax and optimal F0 during both assessments were trivial to moderate (SMD = 0.03-0.82, P = 0.021-0.907). CONCLUSION: These results highlight senior and academy players present with different FV profiles and highlight some potential developmental opportunities for senior and academy rugby league players that sport scientists, strength and conditioning and rugby coaches can implement when designing programmes and considering long-term athlete development.
    • What is PE and who should teach it? Undergraduate PE students’ views and experiences of the outsourcing of PE in the UK

      McEvilly, Nollaig; University of Chester
      This paper investigates beginning BSc Physical Education (PE) students’ views and experiences of the outsourcing of PE in the UK. Outsourcing involves the provision of PE by external providers such as sports coaches. PE in the UK (and other neoliberal Western contexts) is a site in which outsourcing has become increasingly normalised. I anticipated that, as first year undergraduates, the participants would have relatively recent experience of outsourcing as pupils/students (i.e. experience of receiving outsourced PE provision). Data were generated through written narratives (n = 16), completed on the participants’ first day at university, and follow-up semi-structured interviews (n = 10). Drawing on a Foucaultian theoretical framework, I employed a poststructural type of discourse analysis concerned with analysing patterns in language. Referring to PE, the participants drew heavily on a sport discourse, often conflating PE and sport and emphasising the necessity of teachers having knowledge and experience of sports content and skills, as well as a pedagogical discourse (and, to a lesser extent, a ‘healthy lifestyles’ discourse). The participants had a range of experiences of outsourcing in PE, particularly at primary level. They were in favour of primary PE being taught by either specialist PE teachers, or sports coaches (rather than generalist teachers alone). They spoke positively about their experiences of external provision at both primary and secondary school. While few critical comments were provided, participants raised concerns about external providers’ pedagogical knowledge, and some questioned if teachers might feel devalued by external providers being brought in to teach aspects of their curriculum. In general, while the participants recognised teachers’ pedagogical expertise, they also valued external providers’ perceived content knowledge and sporting experience. As such, by conflating PE and sport, they considered that PE should be taught by sport ‘experts’ and that external providers could enhance schools’ internal capabilities.
    • Social Network Analysis of small social groups: application of a hurdle GLMM approach in the Alpine marmot (Marmota marmota)

      Stanley, Christina; von Hardenberg, Achaz; Panaccio, Matteo; Ferrari, Caterina; Bassano, Bruno; University of Chester; University of Turin; Alpine Wildlife Research Centre, Gran Paradiso National Park
      Social Network Analysis (SNA) has recently emerged as a fundamental tool to study animal behavior. While many studies have analyzed the relationship between environmental factors and behavior across large, complex animal populations, few have focused on species living in small groups due to limitations of the statistical methods currently employed. Some of the difficulties are often in comparing social structure across different sized groups and accounting for zero-inflation generated by analyzing small social units. Here we use a case study to highlight how Generalized Linear Mixed Models (GLMMs) and hurdle models can overcome the issues inherent to study of social network metrics of groups that are small and variable in size. We applied this approach to study aggressive behavior in the Alpine marmot (Marmota marmota) using an eight-year long dataset of behavioral interactions across 17 small family groups (7.4 ± 3.3 individuals). We analyzed the effect of individual and group-level factors on aggression, including predictors frequently inferred in species with larger groups, as the closely related yellow-bellied marmot (Marmota flaviventris). Our approach included the use of hurdle GLMMs to analyze the zero-inflated metrics that are typical of aggressive networks of small social groups. Additionally, our results confirmed previously reported effects of dominance and social status on aggression levels, thus supporting the efficacy of our approach. We found differences between males and females in terms of levels of aggression and on the roles occupied by each in agonistic networks that were not predicted in a socially monogamous species. Finally, we provide some perspectives on social network analysis as applied to small social groups to inform subsequent studies.
    • Comment on PP2A inhibition sensitizes cancer stem cells to ABL tyrosine kinase inhibitors in BCR-ABL human leukemia

      Perrotti, D; Agarwal, A; Lucas, Claire; Narla, g; Nevanini, p; Odero, m; Ruvolo, p; Verrills, n; University of Maryland; Imperial College London; Oregon Health and Science University; University of Chester; University of Michigan; University of Southern California; University of Navarra; MD Anderson Cancer Center; University of Newcastle (AAAS, 2019-07-17)
      LB100 does not sensitize CML stem cells to tyrosine kinase inhibitor–induced apoptosis.
    • Discovery of a Novel CIP2A Variant (NOCIVA) with clinical relevance in predicting TKI resistance in myeloid leukemias

      Makela, Eleonora; Pavic, Karolina; Varila, Taru M; Salmenniemi, Urpu; Löyttyniemi, Eliisa; Nagelli, Srikar G; Ammunét, Tea; Kähäri, Veli-Matti; Clark, Richard E; Elo, Laura L; et al.
      Purpose: Cancerous inhibitor of PP2A (CIP2A) is an oncoprotein that inhibits the tumor suppressor PP2A-B56a. However, CIP2A mRNA variants remain uncharacterized. Here, we report the discovery of a CIP2Asplicing variant, NOCIVA (NOvel CIp2a VAriant). Experimental Design: Characterization of CIP2A variants was performed by both 3' and 5' rapid amplification of cDNA ends from cancer cells. The function of NOCIVA was assessed by structural and molecular biology approaches. Its clinical relevance was studied in an acute myeloid leukemia (AML) patient cohort and two independent chronic myeloid leukemia (CML) cohorts. Results: NOCIVA contains CIP2A exons 1-13 fused to 349 nucleotides from CIP2A intron 13. Intriguingly, the first 39 nucleotides of the NOCIVA-specific sequence are in the coding frame with exon 13 of CIP2A and code for a 13 amino acid peptide tail nonhomologous to any known human protein sequence. Therefore, NOCIVA translates to a unique human protein. NOCIVA retains the capacity to bind to B56a, but whereas CIP2A is predominantly a cytoplasmic protein, NOCIVA translocates to the nucleus. Indicative of prevalent alternative splicing from CIP2A to NOCIVA in myeloid malignancies, AML and CML patient samples overexpress NOCIVA but not CIP2A mRNA. In AML, a high NOCIVA/CIP2A mRNA expression ratio is a marker for adverse overall survival. In CML, high NOCIVA expression is associated with inferior event-free survival among imatinib-treated patients, but not among patients treated with dasatinib or nilotinib. Conclusions: We discovered novel variant of the oncoprotein CIP2A and its clinical relevance in predicting tyrosine kinase inhibitor therapy resistance in myeloid leukemias.
    • Low body fat does not influence recovery after muscle-damaging lower-limb plyometrics in young male team sport athletes

      Fernandes, John; Lamb, Kevin; Twist, Craig; University of Chester
      Aim: This study assessed the influence of fat mass to fat-free mass ratio (FM:FFM) on recovery from plyometric exercise. Method: After assessment of body composition, 20 male team sport players (age 20.7 1.1 years; body mass 77.1 11.5 kg) were divided into low- (n = 10; 0.11 0.03) and normal- (n = 10; 0.27 0.09) fat groups based on FM:FFM ratio. Thereafter, participants completed measurements of knee extensor torque at 60 and 240 s􀀀1, countermovement jump flight time, plasma creatine kinase (CK) activity and perceived muscle soreness (VAS) before and at 0, 24 and 48 h after 10 10 maximal plyometric vertical jumps. Results: Evidence of muscle damage was confirmed by alterations in VAS, peak torque at 60 and 240 s􀀀1 and flight time at 0, 24 and 48 h after plyometric exercise (P < 0.05). CK was increased at 0 and 24 h (P < 0.05) but returned to baseline values by 48 h. No time by group e ects were observed for any of the dependent variables (P > 0.05). Conclusion: The current findings indicate that while muscle damage was present after plyometric exercise, the magnitude was similar across the two body composition groups. Applied practitioners can allow for a similar recovery time after plyometric exercise in those with low and normal body fat.
    • High speed running and repeated sprinting in male academy football players

      Twist, Craig; Gibson, Neil V (University of Chester, 2019-08)
      High speed running and repeated sprinting are component parts of training and match play among academy football players. Despite players having to self-pace running speed and the intervening recovery periods during match play, the way these qualities are trained and tested are often externally regulated with specific work-to-rest ratios and prescribed intensities. The aims of this thesis were to investigate high speed running separated by externally regulated and self-selected recovery periods under conditions that replicate training and testing practices analogous with football. Under controlled conditions replicating training practices common amongst academy players, Chapter 4 showed that high speed running and repeated sprinting separated by externally regulated recovery periods resulted in running speeds that differed by a smaller magnitude than those used in their prescription. These data question the fidelity of this approach and the ability of players to replicate prescribed running speeds in the field. Data from Chapter 4 also demonstrated that neuromuscular function was likely reduced 14 hours after high speed running (-5.6%; ES –0.44 ± 0.32; P = 0.01) and combination running (-6.8%; ES -0.53 ± 0.47; P = 0.07) . During 10 x 30 m repeated sprints there was a most likely higher percentage decrement (65%; 0.36 ± 0.21; P = 0.12) and most likely increased physiological load evidenced by between sprint heart rate recovery (-58.9%; ES -1.10 ± 0.72; P = 0.05) when sprints were interspersed by self-selected compared to externally regulated recovery periods (Chapter 5). Performance decrements were, however, attenuated in more mature players (Chapter 6). When considering biological maturity, prePHV players displayed a lower percentage decrement (2.1 ± 1.1%) than post-PHV (3.2 ± 2.1%) players across all sprints when recovery periods were externally regulated (37%; ES 0.41 ± 0.51; P = 0.03). When self-selected recovery periods were used, percentage decrement was lower in the post-PHV group. In Chapter 7, ratings of perceived exertion were used to guide 4 running speed and recovery distribution during a high speed running test performed to volitional exhaustion. Peak running speed in the self-paced (21.8 ± 1.4 km·h-1 ) was likely (4.1%: ES 0.63 ± 0.43; P = 0.03) higher than in the externally regulated YYIRT1 (20.9 ± 1.1 km·h-1); however, average running speed in the self-paced (13.5 ± 1.2 km·h-1) was likely (6.5%; ES 0.67 ± 0.51; P = 0.05) slower (12.7 ± 1.6 km·h-1). There was a moderate difference in total between shuttle recovery periods (13.3%; ES 0.58 ± 0.81; P = 0.16) in the self-paced (552 ± 132 s) compared to externally regulated versions (634 ± 125 s) of the YYIRT1. When exposed to running drills separated by self-selected and externally regulated recovery periods, academy footballers allocate insufficient recovery to preserve running performance and are unable to differentiate between sprinting and high speed running when prescribed according to specific speeds (Chapter 4) and subjective ratings of exertion (Chapter 7). Prescribing self-paced high intensity running interspersed with self-selected recovery periods results in higher physiological loads when compared to externally regulated recovery intermissions and therefore should be considered during training programmes that target adaptations in aerobic capacity. Despite this, where coaches are using high speed running programmes to improve speed and/or speed endurance, externally regulated recoveries are likely to result in the preservation of performance across the repetition range and, as such, are more beneficial to the intended adaptation.
    • Leading brand products and their supermarket economy line equivalents, is there a difference in nutritional content?

      Mushtaq, Sohail; Jackson, Emma; University of Chester
      Since the introduction of supermarket economy lines (SELs) in the early 1990s, their popularity has been established nationwide(1).However, these economical alternatives are commonly perceived to be of lower nutritional quality than their leading brand (LB)equivalents(2,3,4). The present study aimed to determine if there is a significant difference in nutritional content between the UKtop-selling LBs and their SEL equivalents. Additionally, the study aimed to investigate if on average, LBs or SELs provide better‘value for money’.The LBs of 38 most popular food categories were identified from UK market research, and equivalent SEL products were identifiedfrom each of the retailers with the top-five majority UK market share: Tesco, Sainsbury’s, Asda, Morrisons and Aldi. In each foodcategory, differences between LBs and SELs in: energy, fat, saturated fatty acid, carbohydrate, sugar,fibre, protein and salt content,per 100 g of food product were determined using a one-sample T-test. The nutritional quality of each product was also determined bya nutrient profiling system. Cost was analysed in relation to shopping baskets containing 33 equivalent products. Six shopping basketswere analysed, one containing LB products and one from each SEL retailer. The cost of each shopping basket was calculated usingpack price and price per 100 g or 100mL of food product.Data was collected for 219 products; 38 LBs and 181 SELs. 86 significant differences were identified in specific nutrients across thefood categories, but the direction of the differences was inconsistent. Based on pack price, the total LB shopping basket cost was£61·91 whereas average SEL basket cost £28·62, a difference of £33·29 or 54 % (P = 0·001). However, there was no difference betweenthe nutrient profile of LBs and SELs.Although significant differences were identified between nutrients in some food categories, overall, there appeared to be no differ-ence in nutritional content between LBs and SEL equivalents. This association is consistent with previous studies and is contrary tothe common perception that SELs are of lower nutritional quality than LBs(2,3,4,5,6). Pertinent to public health, the present studyfound that SEL breakfast cereals contained a significantly higher amount of salt than the LB (P = 0·035)(4,6). Additionally, althoughthe majority of food categories did not show a significant difference in energy content per 100 g of food product (29 of 38) LB pastahad significantly higher energy content per 100 g of food product than SEL equivalents (P = 0·017)(6).In conclusion, there appears to be no difference in nutritional content between the LB and SEL equivalents in 38 popular foodcategories, however, there appears to be twofold difference in price The cost analysis demonstrates that consumers can purchasethe same quantity of foodstuff for significantly less when opting for SEL products. Low income households may therefore be encour-aged to purchase SEL products to reduce weekly household expenditure and enable a greater proportion of the budget to be availablefor the purchase fresh produce such as fruit, vegetables and meat
    • Estimates of fibre intake and percentage of the population with intake below the dietary reference values (DRVs) in England (1991–2015)

      Mushtaq, Sohail; Farzad, Amirabdollahian; Buczkowski, Bartek; Davies, Ian; University of Chester
      In 1991, the Committee on Medical Aspects of Foods (COMA) defined dietary fibre as non-starch polysaccharides (NSP) and set the DRV as the population average intake of 18 g/day 1 , determined using the Englyst method of analysis 2 . The latest publication of the Scientific Advisory Committee on Nutrition (SACN) 3 broadened the definition of dietary fibre beyond NSP to broader definition of Association of Analytical Communities (AOAC) fibre, recommending the DRV to be 30 g/day based on AOAC method. The COMA 1991, DRV of 18 g/day of NSP corresponds to around 24 g/day of AOAC fibre 3 and therefore the new DRV of fibre would represent a higher recommendation (around 22·5 g fibre as per the Englyst method) for the average population. The purpose of this study was to investigate variation in fibre intake of English population by age and gender, in comparison with the COMA and SACN DRVs. Data on the core sample of the National Diet and Nutrition Survey rolling programme from 2008–2012 was reanalysed. Children aged below 16 years were excluded in consideration of their different DRVs. The data on dietary fibre was extracted from fully productive individuals (i.e. participants who completed three/four diary days), as an average daily intake based on the NSP/Englyst fibre. Inferential statistics included the analysis of variance to discover if there were any significant variations in fibre intake of males and females in relation to their age groups. The statistical significance was set at 0·05. For all age groups, the average fibre intake is below the DRVs. The average daily fibre intake slightly increased with age for both genders until 64 years. When differences in energy intake were taken into account, the average daily fibre density (g/1000 kcal) still increased with the age of participants. Overall, less than a third of populations had an intake above the COMA DRV 1 . More than 90 % of the population had intake below the SACN DRV 3 , demonstrating a challenge for future policies to meet the nutritional guidelines, particularly amongst females and younger adults. The findings should be treated with caution considering the definition of AOAC fibre used as the basis for the SACN DRV includes non-digestible oligosaccharides, resistant starch and polydextrose, going beyond NSP/Englyst variables analysed.
    • Full fat cheese intake and cardiovascular health: a randomised control trial

      Mushtaq, Sohail; Butler, Thomas; Davies, Ian; University of Chester
      Milk and milk products contribute approximately 22 % of the nation's saturated fat (SFA) intake. Recently, the role of dairy and its SFA composition and link to cardiovascular disease (CVD) has been analysed( 1 ), suggesting a beneficial action of this food group on reducing cardiovascular risk in high-risk groups( 2 , 3 ). The aim of this study was to examine the effects of 4 weeks full-fat cheese on circulating lipoprotein fractions, blood pressure and arterial stiffness in healthy adults. Participants were recruited in the city of Chester, UK. Those meeting entry criteria of: 18–65 years of age, not taking antihypercholesterolaemic or antihypertensive medication took part in the study. Participants were randomised to receive either 50 g of a full-fat Red Leicester (FFC) or placebo (virtually zero fat Cheddar cheese, ZFC) per day for 4 weeks. Anthropometry, blood pressure, brachial and aortic augmentation index (BAIX and AAIX, respectively), pulse-wave velocity (PWV) and a full lipid profile were determined at baseline and post-intervention. Participants were asked to keep a 3-day food diary prior to and for the last 3 days of the protocol. All procedures were approved by the Faculty of Medicine, Dentistry and Life Sciences Research Ethics Committee at the University of Chester. Eighty-six (86) individuals completed the study (43 per group). No significant changes were observed in any measured parameter (Table 1). Both ZFC and FFC groups showed a significant increase in calcium intake during the course of the study (1002·1 ± 639·1 mg to 1815·0 ± 1340·1 mg and 1219·6 ± 1169·1 mg 1845·8 ± 1463·2 mg, P < 0·001, respectively) showing good adherence to the protocol. In conclusion, these results suggest that inclusion of 50 g full fat cheese into the diet of a healthy population does not impact negatively on traditional CVD risk markers. Future strategies to reduce SFA intake should focus on – and acknowledge the importance of the source – of SFA in the diet.
    • Effect of a single serving of pecan nuts on blood lipids and weight: a single blind randomised control trial

      Mushtaq, Sohail; Butler, Thomas; Confue, Charlotte; Guild, Joanne; University of Chester
      Nuts are a common component of many traditional cardioprotective diets primarily due to their ability to lower blood lipids and reduce cardiovascular risk(1, 2). Studies consistently show nut intake is associated with favourable changes in energy balance(3). However there is a paucity of data examining the acute changes following nut consumption. We sought to examine the effect of a single serving of pecan nuts on plasma lipids and bodyweight. Participants were sampled from the University of Chester, UK. Individuals (n = 54) were screened for eligibility to participate. Those meeting entry criteria (n = 25) of being either male or female aged 30 years or more and with no previous history of CVD were randomised to either a control (CON) or pecan nut group (PECAN). Participants in the PECAN group received a single 50 g serving of pecan nuts. Capillary blood was taken for analysis of triacylglycerol, total-cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein-cholesterol and non-high density lipoprotein cholesterol (TAG, TC, LDL-C, HDL-C and non-HDL-C, respectively), and anthropometric measurements were performed. All measurements were repeated after 3 days. Participants were instructed to record all food and drink consumed, and not to change their habitual eating habits. Procedures were approved by the Faculty of Medicine, Dentistry and Clinical Sciences Research Ethics Committee, University of Chester. No significant effect on TC, HDL-C or TAG was observed during the study (Fig. 1A–C). LDL-C decreased by 0.09 ± 0.37 mmol/L and increased by 0.16 ± 0.40 mmol/L in CON and PECAN groups, respectively. Non-HDL-C showed a similar pattern with the CON group showing a decrease and PECAN group displaying an increase (−0.18 ± 0.36 mmol/L vs. 0.16 ± 0.40 mmol/L, respectively). Bodyweight significantly (P = 0.025) decreased in the PECAN group when compared to the CON group (−0.58 ± 0.56 kg vs. −0.05 ± 0.55 kg, respectively). In conclusion, a single serving of pecan nuts had no significant impact on lipid markers of cardiovascular risk. Bodyweight was significantly reduced consistent with recent literature showing a favourable relationship with nut intake and energy balance(3).