Browsing Faculty of Medicine, Dentistry and Life Sciences by Authors
Metabolic demands and replenishment of muscle glycogen after a rugby league match simulation protocol.Bradley, Warren J.; Hannon, Marcus P.; Benford, Victoria; Morehen, James C.; Twist, Craig; Shepherd, Sam; Cocks, Matthew; Impey, Samuel G.; Cooper, Robert G.; Morton, James P.; et al. (2017-02-22)Objectives: The metabolic requirements of a rugby league match simulation protocol and the timing of carbohydrate provision on glycogen re-synthesis in damaged muscle were examined. Design: Fifteen (mean ± SD: age 20.9 ± 2.9 y, body-mass 87.3 ± 14.1 kg, height 177.4 ± 6.0 cm) rugby league (RL) players consumed a 6 g•kg•day-1 CHO diet for 7-days, completed a time to exhaustion test (TTE) and a glycogen depletion protocol on day-3, a RL simulated-match protocol (RLMSP) on day-5 and a TTE on day-7. Players were prescribed an immediate or delayed (2-h-post) re-feed post-simulation. Methods: Muscle biopsies and blood samples were obtained post-depletion, before and after simulated match-play, and 48-h after match-play with PlayerLoad and heart-rate collected throughout the simulation. Data were analysed using effects sizes ± 90% CI and magnitude-based inferences. Results: PlayerLoad (8.0 ± 0.7 AU•min-1) and %HRpeak (83 ± 4.9%) during the simulation were similar to values reported for RL match-play. Muscle glycogen very likely increased from immediately after to 48-h post-simulation (272 ± 97 cf. 416 ± 162 mmol•kg-1d.w.; ES ± 90%CI) after immediate re-feed, but changes were unclear (283 ± 68 cf. 361 ± 144 mmol•kg-1d.w.; ES ± 90%CI) after delayed re-feed. CK almost certainly increased by 77.9 ± 25.4% (0.75 ± 0.19) post-simulation for all players. Conclusions: The RLMSP presents a replication of the internal loads associated with professional RL match-play, although difficulties in replicating the collision reduced the metabolic demands and glycogen utilisation. Further, it is possible to replete muscle glycogen in damaged muscle employing an immediate re-feed strategy.
Muscle glycogen utilisation during Rugby match play: Effects of pre-game carbohydrateBradley, Warren J.; Morehen, James C.; Haigh, Julian; Clarke, Jon; Donovan, Timothy F.; Twist, Craig; Cotton, Caroline; Shepherd, Sam; Cocks, Matthew; Sharma, Asheesh; et al. (Elsevier, 2016-04-22)Objectives: Although the physical demands of Rugby League (RL) match-play are well-known, the fuel sources supporting energy-production are poorly understood. We therefore assessed muscle glycogen utilisation and plasma metabolite responses to RL match-play after a relatively high (HCHO) or relatively low CHO (LCHO) diet. Design: Sixteen (mean ± SD age; 18 ± 1 years, body-mass; 88 ± 12 kg, height 180 ± 8 cm) professional players completed a RL match after 36-h consuming a non-isocaloric high carbohydrate (n = 8; 6 g kg day−1) or low carbohydrate (n = 8; 3 g kg day−1) diet. Methods: Muscle biopsies and blood samples were obtained pre- and post-match, alongside external and internal loads quantified using Global Positioning System technology and heart rate, respectively. Data were analysed using effects sizes ±90% CI and magnitude-based inferences. Results: Differences in pre-match muscle glycogen between high and low carbohydrate conditions (449 ± 51 and 444 ± 81 mmol kg−1 d.w.) were unclear. High (243 ± 43 mmol kg−1 d.w.) and low carbohydrate groups (298 ± 130 mmol kg−1 d.w.) were most and very likely reduced post-match, respectively. For both groups, differences in pre-match NEFA and glycerol were unclear, with a most likely increase in NEFA and glycerol post-match. NEFA was likely lower in the high compared with low carbohydrate group post-match (0.95 ± 0.39 mmol l−1 and 1.45 ± 0.51 mmol l−1, respectively), whereas differences between the 2 groups for glycerol were unclear (98.1 ± 33.6 mmol l−1 and 123.1 ± 39.6 mmol l−1) in the high and low carbohydrate groups, respectively. Conclusions: Professional RL players can utilise ∼40% of their muscle glycogen during a competitive match regardless of their carbohydrate consumption in the preceding 36-h.