Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance
dc.contributor.author | Tickle, Jacqueline A. | * |
dc.contributor.author | Jenkins, Stuart I. | * |
dc.contributor.author | Polyak, Boris | * |
dc.contributor.author | Pickard, Mark R. | * |
dc.contributor.author | Chari, Divya M. | * |
dc.date.accessioned | 2016-04-05T15:07:11Z | |
dc.date.available | 2016-04-05T15:07:11Z | |
dc.date.issued | 2016-01-10 | |
dc.identifier.citation | Tickle, J. A., Jenkins, S. I., Polyark, B., Pickard, M. R., & Chari, D. M. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 11(4), 345-358. http://dx.doi.org/10.2217/nnm.15.202 | |
dc.identifier.issn | 1743-5889 | en |
dc.identifier.pmid | 26785794 | |
dc.identifier.doi | 10.2217/nnm.15.202 | |
dc.identifier.uri | http://hdl.handle.net/10034/604463 | |
dc.description.abstract | AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization. | |
dc.description.sponsorship | BBSRC, UK; National Heart, Lung and Blood Institute and Drexel University College of Medicine Clinical & Translational Research Institute, CTRIUSA Award Number R01HL107771; EPSRC E-TERM Landscape Fellowship (EP/I017801/1) | |
dc.language.iso | en | en |
dc.publisher | Future Medicine | |
dc.relation.url | http://www.futuremedicine.com/doi/10.2217/nnm.15.202 | en |
dc.subject | astrocytes | en |
dc.subject | cell transplantation | en |
dc.subject | label dilution | en |
dc.subject | magnetite | en |
dc.subject | magnetolabeling | en |
dc.subject | polymeric particles | en |
dc.title | Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance | en |
dc.type | Article | en |
dc.identifier.eissn | 1748-6963 | en |
dc.contributor.department | Keele University, United Kingdom; Drexel University College of Medicine, Philadelphia, USA | |
dc.identifier.journal | Nanomedicine | en |
html.description.abstract | AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization. |